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Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

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Effect of CD4 depletion on MmmSC-induced recall proliferation (a) and IFN-γ (b) responses of pbmc collected from vaccinated animals.Cells were incubated in the absence (NoAg) or presence of MmmSC Ags (MmmSC), or in the presence of the mitogen Concanavalin A (ConA) as a positive control. Results are expressed as mean percentages (± standard deviation) from three animals.
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pone-0057509-g003: Effect of CD4 depletion on MmmSC-induced recall proliferation (a) and IFN-γ (b) responses of pbmc collected from vaccinated animals.Cells were incubated in the absence (NoAg) or presence of MmmSC Ags (MmmSC), or in the presence of the mitogen Concanavalin A (ConA) as a positive control. Results are expressed as mean percentages (± standard deviation) from three animals.

Mentions: In vaccinated animals showing the strongest T-cell responses, MmmSC-induced recall proliferation was evident among cells that did not belong to the CD4 sub-type although to a lesser extent than in the CD4+ population. Given the potential inhibitory properties of CD4+ T lymphocytes (i.e., Tregulatory or Treg) it was of interest to analyse immune responses occuring in their absence. Therefore, depletion studies were performed and clearly indicated that both proliferation and IFN-γ production induced by MmmSC Ags required the presence of CD4+ T lymphocytes (Fig. 3).


Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Effect of CD4 depletion on MmmSC-induced recall proliferation (a) and IFN-γ (b) responses of pbmc collected from vaccinated animals.Cells were incubated in the absence (NoAg) or presence of MmmSC Ags (MmmSC), or in the presence of the mitogen Concanavalin A (ConA) as a positive control. Results are expressed as mean percentages (± standard deviation) from three animals.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585371&req=5

pone-0057509-g003: Effect of CD4 depletion on MmmSC-induced recall proliferation (a) and IFN-γ (b) responses of pbmc collected from vaccinated animals.Cells were incubated in the absence (NoAg) or presence of MmmSC Ags (MmmSC), or in the presence of the mitogen Concanavalin A (ConA) as a positive control. Results are expressed as mean percentages (± standard deviation) from three animals.
Mentions: In vaccinated animals showing the strongest T-cell responses, MmmSC-induced recall proliferation was evident among cells that did not belong to the CD4 sub-type although to a lesser extent than in the CD4+ population. Given the potential inhibitory properties of CD4+ T lymphocytes (i.e., Tregulatory or Treg) it was of interest to analyse immune responses occuring in their absence. Therefore, depletion studies were performed and clearly indicated that both proliferation and IFN-γ production induced by MmmSC Ags required the presence of CD4+ T lymphocytes (Fig. 3).

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

Show MeSH
Related in: MedlinePlus