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Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

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MmmSC-induced recall proliferative responses of pbmc (a), and CD4+ T cells (b), collected from nonvaccinated (open circles) and vaccinated (closed circles) animals (n = 5) one month after single, double and triple vaccine inoculations.Results represent the net effect of MmmSC stimulation (i.e., stimulated cultures minus non-stimulated cultures) and are representative of 2 independent experiments. Bars indicate median values and asterisks represent significant difference between vaccinated and naïve groups.
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pone-0057509-g001: MmmSC-induced recall proliferative responses of pbmc (a), and CD4+ T cells (b), collected from nonvaccinated (open circles) and vaccinated (closed circles) animals (n = 5) one month after single, double and triple vaccine inoculations.Results represent the net effect of MmmSC stimulation (i.e., stimulated cultures minus non-stimulated cultures) and are representative of 2 independent experiments. Bars indicate median values and asterisks represent significant difference between vaccinated and naïve groups.

Mentions: At individual levels, MmmSC-specific recall proliferation in vaccinated cattle could only be detected in animals that received three shots of T1 vaccines (Fig. 1). However, at group levels, these vaccinated cattle could not be discriminated from nonvaccinated animals when recall proliferation of total PBMC was assessed (Fig. 1a). It is only when proliferation among CD4+ T lymphocytes was measured that significantly (P = 0.03) higher responses were detected in the vaccinated group versus the nonvaccinated group (Fig. 1b).


Characterization of anamnestic T-cell responses induced by conventional vaccines against contagious bovine pleuropneumonia.

Totte P, Yaya A, Sery A, Wesonga H, Wade A, Naessens J, Niang M, Thiaucourt F - PLoS ONE (2013)

MmmSC-induced recall proliferative responses of pbmc (a), and CD4+ T cells (b), collected from nonvaccinated (open circles) and vaccinated (closed circles) animals (n = 5) one month after single, double and triple vaccine inoculations.Results represent the net effect of MmmSC stimulation (i.e., stimulated cultures minus non-stimulated cultures) and are representative of 2 independent experiments. Bars indicate median values and asterisks represent significant difference between vaccinated and naïve groups.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585371&req=5

pone-0057509-g001: MmmSC-induced recall proliferative responses of pbmc (a), and CD4+ T cells (b), collected from nonvaccinated (open circles) and vaccinated (closed circles) animals (n = 5) one month after single, double and triple vaccine inoculations.Results represent the net effect of MmmSC stimulation (i.e., stimulated cultures minus non-stimulated cultures) and are representative of 2 independent experiments. Bars indicate median values and asterisks represent significant difference between vaccinated and naïve groups.
Mentions: At individual levels, MmmSC-specific recall proliferation in vaccinated cattle could only be detected in animals that received three shots of T1 vaccines (Fig. 1). However, at group levels, these vaccinated cattle could not be discriminated from nonvaccinated animals when recall proliferation of total PBMC was assessed (Fig. 1a). It is only when proliferation among CD4+ T lymphocytes was measured that significantly (P = 0.03) higher responses were detected in the vaccinated group versus the nonvaccinated group (Fig. 1b).

Bottom Line: A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP).Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment.In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

View Article: PubMed Central - PubMed

Affiliation: Centre International de Recherche en Agronomie pour le Développement, UMR CMAEE, Montpellier, France. philippe.totte@cirad.fr

ABSTRACT
A better understanding of how T1 vaccination confers immunity would facilitate the rational design of improved vaccines against contagious bovine pleuropneumonia (CBPP). We show here that mycoplasmas-induced recall proliferation and IFN-γ responses are detected in cattle that received multiple shots of T1 vaccines. These anamnestic responses were under the strict control of CD4(+) T lymphocytes. Moreover, CD62L expression indicated that both CD4(+) effector memory (Tem) and central memory (Tcm) T lymphocytes are elicited in these animals. Comparative analysis with data from cattle that completely recovered from CBPP infection revealed similar anamnestic T-cell responses albeit at a lower magnitude for T1-vaccinated animals, particularly in the Tcm compartment. In conclusion, we discuss how our current understanding of T-cell responses will contribute to ongoing efforts for the improvement of future CBPP vaccines.

Show MeSH
Related in: MedlinePlus