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Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.

Zhang Z, Zhang Q, Wu J, Zheng X, Zheng S, Sun X, Qiu Q, Lu T - PLoS ONE (2013)

Bottom Line: Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility.H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments.Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key facility for Gene Resources And Genetic Improvement, Beijing, China.

ABSTRACT
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H2O2 into H2O and O2, play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

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Related in: MedlinePlus

The effects of stress treatments on wild-type and Osapx2 plants.A: Relative water content. B: Chlorophyll content. C: H2O2 content. D: MDA content. Values represent the mean ± SD of three replicates.
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pone-0057472-g010: The effects of stress treatments on wild-type and Osapx2 plants.A: Relative water content. B: Chlorophyll content. C: H2O2 content. D: MDA content. Values represent the mean ± SD of three replicates.

Mentions: The responses of Osapx2 mutant, wild-type and OsAPX2-OX plants to abiotic stresses are consistence to their relative water content, chlorophyll content, H2O2 and MDA contents. The abiotic stress sensitive phenotype of Osapx2 was consistent with more water loss, lower chlorophyll content, higher H2O2 and MDA contents after stress treatments (Figure 10). By contrast, the abiotic stress tolerance phenotype of OsAPX2-OX was consistent with less water loss, higher chlorophyll content, and lower H2O2 and MDA contents (Figure 11).


Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.

Zhang Z, Zhang Q, Wu J, Zheng X, Zheng S, Sun X, Qiu Q, Lu T - PLoS ONE (2013)

The effects of stress treatments on wild-type and Osapx2 plants.A: Relative water content. B: Chlorophyll content. C: H2O2 content. D: MDA content. Values represent the mean ± SD of three replicates.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585366&req=5

pone-0057472-g010: The effects of stress treatments on wild-type and Osapx2 plants.A: Relative water content. B: Chlorophyll content. C: H2O2 content. D: MDA content. Values represent the mean ± SD of three replicates.
Mentions: The responses of Osapx2 mutant, wild-type and OsAPX2-OX plants to abiotic stresses are consistence to their relative water content, chlorophyll content, H2O2 and MDA contents. The abiotic stress sensitive phenotype of Osapx2 was consistent with more water loss, lower chlorophyll content, higher H2O2 and MDA contents after stress treatments (Figure 10). By contrast, the abiotic stress tolerance phenotype of OsAPX2-OX was consistent with less water loss, higher chlorophyll content, and lower H2O2 and MDA contents (Figure 11).

Bottom Line: Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility.H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments.Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key facility for Gene Resources And Genetic Improvement, Beijing, China.

ABSTRACT
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H2O2 into H2O and O2, play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

Show MeSH
Related in: MedlinePlus