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Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.

Zhang Z, Zhang Q, Wu J, Zheng X, Zheng S, Sun X, Qiu Q, Lu T - PLoS ONE (2013)

Bottom Line: Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility.H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments.Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key facility for Gene Resources And Genetic Improvement, Beijing, China.

ABSTRACT
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H2O2 into H2O and O2, play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

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The seed phenotype of WT and Osapx2.A,B: the seed phenotype of WT (odd numbers) and Osapx2 (even numbers), before dehulling (A) and after dehulling (B). C: The thousand kernel weight of WT and Osapx2 seeds.
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pone-0057472-g002: The seed phenotype of WT and Osapx2.A,B: the seed phenotype of WT (odd numbers) and Osapx2 (even numbers), before dehulling (A) and after dehulling (B). C: The thousand kernel weight of WT and Osapx2 seeds.

Mentions: In an attempt to obtain drought sensitive rice mutants, we conducted genetic screening of a T-DNA insertion population containing more than 100,000 individual lines using 20% PEG2000. A PEG-sensitive mutant was identified with the phenotype of severe inhibition in the elongation of both shoots and roots (Figure 1). Additionally, the mutant seeds were smaller than the wild-type (Figure 2-A,B). As measured by the thousand kernel weight (TKW) of seeds, the mutant TKW was 17.6±0.3 g while that of wild-type was 24.4±0.4 g (Figure 2-C).


Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.

Zhang Z, Zhang Q, Wu J, Zheng X, Zheng S, Sun X, Qiu Q, Lu T - PLoS ONE (2013)

The seed phenotype of WT and Osapx2.A,B: the seed phenotype of WT (odd numbers) and Osapx2 (even numbers), before dehulling (A) and after dehulling (B). C: The thousand kernel weight of WT and Osapx2 seeds.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585366&req=5

pone-0057472-g002: The seed phenotype of WT and Osapx2.A,B: the seed phenotype of WT (odd numbers) and Osapx2 (even numbers), before dehulling (A) and after dehulling (B). C: The thousand kernel weight of WT and Osapx2 seeds.
Mentions: In an attempt to obtain drought sensitive rice mutants, we conducted genetic screening of a T-DNA insertion population containing more than 100,000 individual lines using 20% PEG2000. A PEG-sensitive mutant was identified with the phenotype of severe inhibition in the elongation of both shoots and roots (Figure 1). Additionally, the mutant seeds were smaller than the wild-type (Figure 2-A,B). As measured by the thousand kernel weight (TKW) of seeds, the mutant TKW was 17.6±0.3 g while that of wild-type was 24.4±0.4 g (Figure 2-C).

Bottom Line: Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility.H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments.Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology Research Institute, Chinese Academy of Agricultural Sciences/National Key facility for Gene Resources And Genetic Improvement, Beijing, China.

ABSTRACT
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H2O2 into H2O and O2, play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.

Show MeSH
Related in: MedlinePlus