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Early and efficient detection of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures.

Kidenya BR, Kabangila R, Peck RN, Mshana SE, Webster LE, Koenig SP, Johnson WD, Fitzgerald DW - PLoS ONE (2013)

Bottom Line: There were a total of 200 (62.3%) pulmonary tuberculosis cases.Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3%) versus 77.0% (95% CI, 71.2% to 82.8%) (p = 0.0007).The cost for micro broth culture (labor inclusive) in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, Tanzania. benkidenya@yahoo.com

ABSTRACT
Early, efficient and inexpensive methods for the detection of pulmonary tuberculosis are urgently needed for effective patient management as well as to interrupt transmission. These methods to detect M. tuberculosis in a timely and affordable way are not yet widely available in resource-limited settings. In a developing-country setting, we prospectively evaluated two methods for culturing and detecting M. tuberculosis in sputum. Sputum samples were cultured in liquid assay (micro broth culture) in microplate wells and growth was detected by microscopic observation, or in Löwenstein-Jensen (LJ) solid media where growth was detected by visual inspection for colonies. Sputum samples were collected from 321 tuberculosis (TB) suspects attending Bugando Medical Centre, in Mwanza, Tanzania, and were cultured in parallel. Pulmonary tuberculosis cases were diagnosed using the American Thoracic Society diagnostic standards. There were a total of 200 (62.3%) pulmonary tuberculosis cases. Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3%) versus 77.0% (95% CI, 71.2% to 82.8%) (p = 0.0007). The median turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ solid culture, 9 days (interquartile range [IQR] 7-13), versus 21 days (IQR 14-28) (p<0.0001). The cost for micro broth culture (labor inclusive) in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture. The liquid assay (micro broth culture) is an early, feasible, and inexpensive method for detection of pulmonary tuberculosis in resource limited settings.

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Related in: MedlinePlus

Time to positive culture for solid Löwenstein–Jensen (LJ) versus liquid broth with microscopic detection.*The comparison of time to growth detection for paired 138 samples which were positive for both micro broth culture and LJ culture.
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pone-0057527-g001: Time to positive culture for solid Löwenstein–Jensen (LJ) versus liquid broth with microscopic detection.*The comparison of time to growth detection for paired 138 samples which were positive for both micro broth culture and LJ culture.

Mentions: The time elapsed from the date of inoculation to the date of positive-culture result availability for each sample was registered as a turnaround time (TAT). The median TAT for micro broth culture was 9 days, with an interquartile range (IQR) of 7–13 days, whereas for LJ it was 21 days, IQR 14–28 days. The 138 samples that were positive for both micro broth culture and LJ were paired and the time to positive culture results were analyzed. The turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ culture (p-value <0.0001) [Figure 1].


Early and efficient detection of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures.

Kidenya BR, Kabangila R, Peck RN, Mshana SE, Webster LE, Koenig SP, Johnson WD, Fitzgerald DW - PLoS ONE (2013)

Time to positive culture for solid Löwenstein–Jensen (LJ) versus liquid broth with microscopic detection.*The comparison of time to growth detection for paired 138 samples which were positive for both micro broth culture and LJ culture.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585352&req=5

pone-0057527-g001: Time to positive culture for solid Löwenstein–Jensen (LJ) versus liquid broth with microscopic detection.*The comparison of time to growth detection for paired 138 samples which were positive for both micro broth culture and LJ culture.
Mentions: The time elapsed from the date of inoculation to the date of positive-culture result availability for each sample was registered as a turnaround time (TAT). The median TAT for micro broth culture was 9 days, with an interquartile range (IQR) of 7–13 days, whereas for LJ it was 21 days, IQR 14–28 days. The 138 samples that were positive for both micro broth culture and LJ were paired and the time to positive culture results were analyzed. The turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ culture (p-value <0.0001) [Figure 1].

Bottom Line: There were a total of 200 (62.3%) pulmonary tuberculosis cases.Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3%) versus 77.0% (95% CI, 71.2% to 82.8%) (p = 0.0007).The cost for micro broth culture (labor inclusive) in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, Tanzania. benkidenya@yahoo.com

ABSTRACT
Early, efficient and inexpensive methods for the detection of pulmonary tuberculosis are urgently needed for effective patient management as well as to interrupt transmission. These methods to detect M. tuberculosis in a timely and affordable way are not yet widely available in resource-limited settings. In a developing-country setting, we prospectively evaluated two methods for culturing and detecting M. tuberculosis in sputum. Sputum samples were cultured in liquid assay (micro broth culture) in microplate wells and growth was detected by microscopic observation, or in Löwenstein-Jensen (LJ) solid media where growth was detected by visual inspection for colonies. Sputum samples were collected from 321 tuberculosis (TB) suspects attending Bugando Medical Centre, in Mwanza, Tanzania, and were cultured in parallel. Pulmonary tuberculosis cases were diagnosed using the American Thoracic Society diagnostic standards. There were a total of 200 (62.3%) pulmonary tuberculosis cases. Liquid assay with microscopic detection detected a significantly higher proportion of cases than LJ solid culture: 89.0% (95% confidence interval [CI], 84.7% to 93.3%) versus 77.0% (95% CI, 71.2% to 82.8%) (p = 0.0007). The median turn around time to diagnose tuberculosis was significantly shorter for micro broth culture than for the LJ solid culture, 9 days (interquartile range [IQR] 7-13), versus 21 days (IQR 14-28) (p<0.0001). The cost for micro broth culture (labor inclusive) in our study was US $4.56 per sample, versus US $11.35 per sample for the LJ solid culture. The liquid assay (micro broth culture) is an early, feasible, and inexpensive method for detection of pulmonary tuberculosis in resource limited settings.

Show MeSH
Related in: MedlinePlus