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Circulating microRNAs and aerobic fitness--the HUNT-Study.

Bye A, Røsjø H, Aspenes ST, Condorelli G, Omland T, Wisløff U - PLoS ONE (2013)

Bottom Line: Candiate miRs were validated in a second cohort of subjects with high (n = 38) or low (n = 38) VO2max. miR-210 and miR-222 were found to be higher in the low VO2max-group (p<0.05).In conclusion, we found that miR-210, miR-21, and miR-222 were increased in healthy subjects with low VO2max.The lack of association between these three miRs, and other fitness related variables as well as traditional CVD risk factors, suggests that these miRs may have a potential as new independent biomarkers of fitness level and future CVD.

View Article: PubMed Central - PubMed

Affiliation: K.G. Jebsen Center of Exercise in Medicine, Department of Circulation and Medical Imaging, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway. Anja.Bye@ntnu.no

ABSTRACT
Aerobic fitness, measured as maximal oxygen uptake (VO2max), is a good indicator of cardiovascular health, and a strong predictor of cardiovascular mortality. Biomarkers associated with low VO2max may therefore represent potential early markers of future cardiovascular disease (CVD). The aim of this study was to assess whether circulating microRNAs (miRs) are associated with VO2max-level in healthy individuals. In a screening study, 720 miRs were measured in serum samples from healthy individuals (40-45 yrs) with high (n = 12) or low (n = 12) VO2max matched for gender, age and physical activity. Candiate miRs were validated in a second cohort of subjects with high (n = 38) or low (n = 38) VO2max. miR-210 and miR-222 were found to be higher in the low VO2max-group (p<0.05). In addition, miR-21 was increased in male participants with low VO2max (p<0.05). There were no correlations between traditional risk factors for CVD (blood pressure, cholesterol, smoking habit, or obesity) and miR-21, miR-210 and miR-222. DIANA-mirPath identified 611 potential gene-targets of miR-21, miR-210 and miR-222, and pathway analysis indicated alterations in several important signaling systems in subjects with low VO2max. Potential bias involve that blood was collected from non-fasting individuals, and that 8 performed exercise within 24 h before sampling. In conclusion, we found that miR-210, miR-21, and miR-222 were increased in healthy subjects with low VO2max. The lack of association between these three miRs, and other fitness related variables as well as traditional CVD risk factors, suggests that these miRs may have a potential as new independent biomarkers of fitness level and future CVD.

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Related in: MedlinePlus

MicroRNAs with significantly different serum levels in individuals with high and low aerobic fitness in the screening cohort (p<0.05).A: In both men and women (n = 24), B: Men (n = 12), C: Women (n = 12). VO2max: Maximal oxygen uptake. Error bars represent 1 standard deviation.
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pone-0057496-g001: MicroRNAs with significantly different serum levels in individuals with high and low aerobic fitness in the screening cohort (p<0.05).A: In both men and women (n = 24), B: Men (n = 12), C: Women (n = 12). VO2max: Maximal oxygen uptake. Error bars represent 1 standard deviation.

Mentions: The baseline characteristics of the populations are displayed in the Table 1. Out of the 720 analyzed miRs, 50 miRs were successfully assessed with sufficient signal in >80% of the samples and qualified for further analyses. Participants with low VO2max had significantly higher levels of miR-210 and miR-125a compared to participants with high VO2max in the screening cohort (ddCp>+/−0.50, p<0.01) (Figure 1A). In addition, subjects with low VO2max had significantly lower serum levels of miR-652 (p<0.001) (Figure 1A). In male participants, low VO2max was associated with reduced levels of miR-151 and increased levels of miR-29a and miR-125a compared to male participants with high VO2max (Figure 1B). In women, miR-210, let-7d and miR-21 were increased in subjects with low VO2max compared to subjects with high VO2max (ddCp>+/−0.50 and p<0.05) (Figure 1C).


Circulating microRNAs and aerobic fitness--the HUNT-Study.

Bye A, Røsjø H, Aspenes ST, Condorelli G, Omland T, Wisløff U - PLoS ONE (2013)

MicroRNAs with significantly different serum levels in individuals with high and low aerobic fitness in the screening cohort (p<0.05).A: In both men and women (n = 24), B: Men (n = 12), C: Women (n = 12). VO2max: Maximal oxygen uptake. Error bars represent 1 standard deviation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585333&req=5

pone-0057496-g001: MicroRNAs with significantly different serum levels in individuals with high and low aerobic fitness in the screening cohort (p<0.05).A: In both men and women (n = 24), B: Men (n = 12), C: Women (n = 12). VO2max: Maximal oxygen uptake. Error bars represent 1 standard deviation.
Mentions: The baseline characteristics of the populations are displayed in the Table 1. Out of the 720 analyzed miRs, 50 miRs were successfully assessed with sufficient signal in >80% of the samples and qualified for further analyses. Participants with low VO2max had significantly higher levels of miR-210 and miR-125a compared to participants with high VO2max in the screening cohort (ddCp>+/−0.50, p<0.01) (Figure 1A). In addition, subjects with low VO2max had significantly lower serum levels of miR-652 (p<0.001) (Figure 1A). In male participants, low VO2max was associated with reduced levels of miR-151 and increased levels of miR-29a and miR-125a compared to male participants with high VO2max (Figure 1B). In women, miR-210, let-7d and miR-21 were increased in subjects with low VO2max compared to subjects with high VO2max (ddCp>+/−0.50 and p<0.05) (Figure 1C).

Bottom Line: Candiate miRs were validated in a second cohort of subjects with high (n = 38) or low (n = 38) VO2max. miR-210 and miR-222 were found to be higher in the low VO2max-group (p<0.05).In conclusion, we found that miR-210, miR-21, and miR-222 were increased in healthy subjects with low VO2max.The lack of association between these three miRs, and other fitness related variables as well as traditional CVD risk factors, suggests that these miRs may have a potential as new independent biomarkers of fitness level and future CVD.

View Article: PubMed Central - PubMed

Affiliation: K.G. Jebsen Center of Exercise in Medicine, Department of Circulation and Medical Imaging, Faculty of Medicine, Norwegian University of Science and Technology, Trondheim, Norway. Anja.Bye@ntnu.no

ABSTRACT
Aerobic fitness, measured as maximal oxygen uptake (VO2max), is a good indicator of cardiovascular health, and a strong predictor of cardiovascular mortality. Biomarkers associated with low VO2max may therefore represent potential early markers of future cardiovascular disease (CVD). The aim of this study was to assess whether circulating microRNAs (miRs) are associated with VO2max-level in healthy individuals. In a screening study, 720 miRs were measured in serum samples from healthy individuals (40-45 yrs) with high (n = 12) or low (n = 12) VO2max matched for gender, age and physical activity. Candiate miRs were validated in a second cohort of subjects with high (n = 38) or low (n = 38) VO2max. miR-210 and miR-222 were found to be higher in the low VO2max-group (p<0.05). In addition, miR-21 was increased in male participants with low VO2max (p<0.05). There were no correlations between traditional risk factors for CVD (blood pressure, cholesterol, smoking habit, or obesity) and miR-21, miR-210 and miR-222. DIANA-mirPath identified 611 potential gene-targets of miR-21, miR-210 and miR-222, and pathway analysis indicated alterations in several important signaling systems in subjects with low VO2max. Potential bias involve that blood was collected from non-fasting individuals, and that 8 performed exercise within 24 h before sampling. In conclusion, we found that miR-210, miR-21, and miR-222 were increased in healthy subjects with low VO2max. The lack of association between these three miRs, and other fitness related variables as well as traditional CVD risk factors, suggests that these miRs may have a potential as new independent biomarkers of fitness level and future CVD.

Show MeSH
Related in: MedlinePlus