Limits...
De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

Show MeSH

Related in: MedlinePlus

Terpenoid pathways represented in the PlantCyc annotation of the representative transcripts.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3585318&req=5

pone-0056217-g007: Terpenoid pathways represented in the PlantCyc annotation of the representative transcripts.

Mentions: It has been suggested that plants have developed terpene based host defence, which also represents a repertoire of therapeutic compounds [38]. Hence in this study, we have focused our analysis towards terpenoids. Major share of transcripts related to terpenoid pathways were found to be from menthol biosynthesis (25%). Recent evidences indicate that menthol has a potent anticancer property, effecting cell death through TRPM8 receptor [39]. Here in this study for the first time, we report the present of genes related to taxol biosynthetic pathway in turmeric. Taxol biosynthetic pathway was the third most represented (8.11%) terpenoid pathway in the transcriptome (Figure 7). Further studies into this pathway in turmeric could be benificial as the combination of taxol with curcumin has produced promising results in treating cancer [40].


De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Terpenoid pathways represented in the PlantCyc annotation of the representative transcripts.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585318&req=5

pone-0056217-g007: Terpenoid pathways represented in the PlantCyc annotation of the representative transcripts.
Mentions: It has been suggested that plants have developed terpene based host defence, which also represents a repertoire of therapeutic compounds [38]. Hence in this study, we have focused our analysis towards terpenoids. Major share of transcripts related to terpenoid pathways were found to be from menthol biosynthesis (25%). Recent evidences indicate that menthol has a potent anticancer property, effecting cell death through TRPM8 receptor [39]. Here in this study for the first time, we report the present of genes related to taxol biosynthetic pathway in turmeric. Taxol biosynthetic pathway was the third most represented (8.11%) terpenoid pathway in the transcriptome (Figure 7). Further studies into this pathway in turmeric could be benificial as the combination of taxol with curcumin has produced promising results in treating cancer [40].

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

Show MeSH
Related in: MedlinePlus