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De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

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Related in: MedlinePlus

Expression profile of the differentially expressed transcripts (A) in cultivar B with respect to A (B) in cultivar C with respect to A.
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pone-0056217-g006: Expression profile of the differentially expressed transcripts (A) in cultivar B with respect to A (B) in cultivar C with respect to A.

Mentions: In this study, cultivar Nattu was used as control and the differential expression of transcripts in Erode and Mysore cultivars were determined. We observed 1,774 and 1,356 differentially expressed transcripts in Erode and Mysore cultivars respectively (Figure 6). Of the 1,774 differentially expressed transcripts in cultivar Erode, we observed 629 upregulated transcripts and 1,145 downregulated transcripts. Similarly, in cultivar Mysore, we found 793 upregulated transcripts and 563 downregulated transcripts. We were not able to find significant expression level differences among the three cultivars at the transcriptome level. However, one significant observation in the expression analysis is that hypericin, a secondary metabolite with antitumor [23], [24] and antibiotic properties [25], was found to be upregulated in both Erode and Mysore cultivars.


De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Expression profile of the differentially expressed transcripts (A) in cultivar B with respect to A (B) in cultivar C with respect to A.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585318&req=5

pone-0056217-g006: Expression profile of the differentially expressed transcripts (A) in cultivar B with respect to A (B) in cultivar C with respect to A.
Mentions: In this study, cultivar Nattu was used as control and the differential expression of transcripts in Erode and Mysore cultivars were determined. We observed 1,774 and 1,356 differentially expressed transcripts in Erode and Mysore cultivars respectively (Figure 6). Of the 1,774 differentially expressed transcripts in cultivar Erode, we observed 629 upregulated transcripts and 1,145 downregulated transcripts. Similarly, in cultivar Mysore, we found 793 upregulated transcripts and 563 downregulated transcripts. We were not able to find significant expression level differences among the three cultivars at the transcriptome level. However, one significant observation in the expression analysis is that hypericin, a secondary metabolite with antitumor [23], [24] and antibiotic properties [25], was found to be upregulated in both Erode and Mysore cultivars.

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

Show MeSH
Related in: MedlinePlus