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De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

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Top ten most expressed Pfam domains.
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pone-0056217-g005: Top ten most expressed Pfam domains.

Mentions: For distantly related homologs, sequence similarity search may not yield significant information, hence transcripts were searched for the presence of conserved protein domains (Additional file S6). Protein kinases (PF00069 and PF07714) were found to be the most abundant domains and given their involvement in a variety of cellular processes, including metabolism, transcription, cell movement, differentiation and apoptosis, their abundance is expected. Annotation revealed the presence of Myb_DNA-binding domain, which is the sixth most occurring domain. This domain is responsible for the transcription factor activity of Myb transcription factor superfamily, which plays regulatory role in plant developmental process and defense responses. Cytochrome P450 superfamily (PF00067) is a large and diverse group of enzymes involved in catalysing the oxidation of organic substrates and the presence of P450 domain as the eighth most occurring domain supports their abundance. The other domains such as PPR_2 repeat family (PF13041), RNA recognition motif (PF00076), RING finger domain (PF13639), Protein phosphatase 2C (PF00481), WD40 (PF00400), PPR (PF01535) were also found aplenty (Figure 5).


De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Top ten most expressed Pfam domains.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585318&req=5

pone-0056217-g005: Top ten most expressed Pfam domains.
Mentions: For distantly related homologs, sequence similarity search may not yield significant information, hence transcripts were searched for the presence of conserved protein domains (Additional file S6). Protein kinases (PF00069 and PF07714) were found to be the most abundant domains and given their involvement in a variety of cellular processes, including metabolism, transcription, cell movement, differentiation and apoptosis, their abundance is expected. Annotation revealed the presence of Myb_DNA-binding domain, which is the sixth most occurring domain. This domain is responsible for the transcription factor activity of Myb transcription factor superfamily, which plays regulatory role in plant developmental process and defense responses. Cytochrome P450 superfamily (PF00067) is a large and diverse group of enzymes involved in catalysing the oxidation of organic substrates and the presence of P450 domain as the eighth most occurring domain supports their abundance. The other domains such as PPR_2 repeat family (PF13041), RNA recognition motif (PF00076), RING finger domain (PF13639), Protein phosphatase 2C (PF00481), WD40 (PF00400), PPR (PF01535) were also found aplenty (Figure 5).

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

Show MeSH
Related in: MedlinePlus