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De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

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Related in: MedlinePlus

Coverage distribution of NCBI C. longa ESTs matched against representative transcripts using BLAST.
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pone-0056217-g002: Coverage distribution of NCBI C. longa ESTs matched against representative transcripts using BLAST.

Mentions: Sequence similarity search between RTs and GenBank's C. longa ESTs showed that 9,307 (15.1%) RTs were similar to 11,139 (86.8%) C. longa ESTs at an E-value cut-off of e-5 (<0.00001). Of these, 11,115 sequences matched with a sequence identity greater than 80% while the remaining sequences matched with an identity above 70%. A vast majority of the ESTs (5,372) were observed to align with more than 90% coverage (Figure 2). This search also revealed the presence of curcumin synthase in the transcriptome (Additional file S1).


De Novo transcriptome assembly (NGS) of Curcuma longa L. rhizome reveals novel transcripts related to anticancer and antimalarial terpenoids.

Annadurai RS, Neethiraj R, Jayakumar V, Damodaran AC, Rao SN, Katta MA, Gopinathan S, Sarma SP, Senthilkumar V, Niranjan V, Gopinath A, Mugasimangalam RC - PLoS ONE (2013)

Coverage distribution of NCBI C. longa ESTs matched against representative transcripts using BLAST.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585318&req=5

pone-0056217-g002: Coverage distribution of NCBI C. longa ESTs matched against representative transcripts using BLAST.
Mentions: Sequence similarity search between RTs and GenBank's C. longa ESTs showed that 9,307 (15.1%) RTs were similar to 11,139 (86.8%) C. longa ESTs at an E-value cut-off of e-5 (<0.00001). Of these, 11,115 sequences matched with a sequence identity greater than 80% while the remaining sequences matched with an identity above 70%. A vast majority of the ESTs (5,372) were observed to align with more than 90% coverage (Figure 2). This search also revealed the presence of curcumin synthase in the transcriptome (Additional file S1).

Bottom Line: Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects.We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical.Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Unit, Genotypic Technology Private Limited, Bangalore, Karnataka, India.

ABSTRACT
Herbal remedies are increasingly being recognised in recent years as alternative medicine for a number of diseases including cancer. Curcuma longa L., commonly known as turmeric is used as a culinary spice in India and in many Asian countries has been attributed to lower incidences of gastrointestinal cancers. Curcumin, a secondary metabolite isolated from the rhizomes of this plant has been shown to have significant anticancer properties, in addition to antimalarial and antioxidant effects. We sequenced the transcriptome of the rhizome of the 3 varieties of Curcuma longa L. using Illumina reversible dye terminator sequencing followed by de novo transcriptome assembly. Multiple databases were used to obtain a comprehensive annotation and the transcripts were functionally classified using GO, KOG and PlantCyc. Special emphasis was given for annotating the secondary metabolite pathways and terpenoid biosynthesis pathways. We report for the first time, the presence of transcripts related to biosynthetic pathways of several anti-cancer compounds like taxol, curcumin, and vinblastine in addition to anti-malarial compounds like artemisinin and acridone alkaloids, emphasizing turmeric's importance as a highly potent phytochemical. Our data not only provides molecular signatures for several terpenoids but also a comprehensive molecular resource for facilitating deeper insights into the transcriptome of C. longa.

Show MeSH
Related in: MedlinePlus