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CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.

Batista MD, Tincati C, Milush JM, Ho EL, Ndhlovu LC, York VA, Kallas EG, Kalil J, Keating SM, Norris PJ, Chang D, Unemori P, Leslie KS, Maurer T, Liao W, Nixon DF - PLoS ONE (2013)

Bottom Line: CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

View Article: PubMed Central - PubMed

Affiliation: Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA. maridiasbatista@gmail.com

ABSTRACT

Background: The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.

Methodology: We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.

Principal findings: We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin.

Conclusions/significance: These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

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Related in: MedlinePlus

Sorted CD4+ T cell cytokine production.Cytokine production by sorted CD4+ T cells from unaffected and lesional skin from psoriasis patients, with stimulation with PMA-ionomycin. Comparative chart representing IL-17A, IL-22, IL-2, IFN-gamma, TNF-alpha and IL-27. * = p<0.05.
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pone-0052144-g003: Sorted CD4+ T cell cytokine production.Cytokine production by sorted CD4+ T cells from unaffected and lesional skin from psoriasis patients, with stimulation with PMA-ionomycin. Comparative chart representing IL-17A, IL-22, IL-2, IFN-gamma, TNF-alpha and IL-27. * = p<0.05.

Mentions: While unstimulated samples from all compartments did not seem to produce significant cytokine levels, we observed that lesional skin CD4+ T cells stimulated with PMA-ionomycin produced higher levels of IL-17A, IL-22, and IFN-gamma in relation to unaffected skin from the same patients (Figure 3). There was a trend to higher production of TNF-alpha and IL-2 by sorted CD4+ T cells from lesional skin, while no difference was observed for IL-27 levels (Figure 3).


CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.

Batista MD, Tincati C, Milush JM, Ho EL, Ndhlovu LC, York VA, Kallas EG, Kalil J, Keating SM, Norris PJ, Chang D, Unemori P, Leslie KS, Maurer T, Liao W, Nixon DF - PLoS ONE (2013)

Sorted CD4+ T cell cytokine production.Cytokine production by sorted CD4+ T cells from unaffected and lesional skin from psoriasis patients, with stimulation with PMA-ionomycin. Comparative chart representing IL-17A, IL-22, IL-2, IFN-gamma, TNF-alpha and IL-27. * = p<0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585296&req=5

pone-0052144-g003: Sorted CD4+ T cell cytokine production.Cytokine production by sorted CD4+ T cells from unaffected and lesional skin from psoriasis patients, with stimulation with PMA-ionomycin. Comparative chart representing IL-17A, IL-22, IL-2, IFN-gamma, TNF-alpha and IL-27. * = p<0.05.
Mentions: While unstimulated samples from all compartments did not seem to produce significant cytokine levels, we observed that lesional skin CD4+ T cells stimulated with PMA-ionomycin produced higher levels of IL-17A, IL-22, and IFN-gamma in relation to unaffected skin from the same patients (Figure 3). There was a trend to higher production of TNF-alpha and IL-2 by sorted CD4+ T cells from lesional skin, while no difference was observed for IL-27 levels (Figure 3).

Bottom Line: CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

View Article: PubMed Central - PubMed

Affiliation: Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA. maridiasbatista@gmail.com

ABSTRACT

Background: The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.

Methodology: We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.

Principal findings: We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin.

Conclusions/significance: These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

Show MeSH
Related in: MedlinePlus