Limits...
CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.

Batista MD, Tincati C, Milush JM, Ho EL, Ndhlovu LC, York VA, Kallas EG, Kalil J, Keating SM, Norris PJ, Chang D, Unemori P, Leslie KS, Maurer T, Liao W, Nixon DF - PLoS ONE (2013)

Bottom Line: CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

View Article: PubMed Central - PubMed

Affiliation: Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA. maridiasbatista@gmail.com

ABSTRACT

Background: The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.

Methodology: We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.

Principal findings: We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin.

Conclusions/significance: These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

Show MeSH

Related in: MedlinePlus

T cell distribution in skin and PBMC of psoriasis patients.Frequency of (A) CD4+ T cells and (B) CD8+ T cells in the skin (lesional and unaffected) from psoriasis patients (n = 7). * = p<0.05.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3585296&req=5

pone-0052144-g001: T cell distribution in skin and PBMC of psoriasis patients.Frequency of (A) CD4+ T cells and (B) CD8+ T cells in the skin (lesional and unaffected) from psoriasis patients (n = 7). * = p<0.05.

Mentions: We first determined whether the CD4+ or CD8+ T cell distribution was altered in unaffected skin compared to lesional skin of psoriasis patients. CD45+ leukocytes in skin samples (psoriatic lesions and non-lesional) were assessed by flow cytometry. We observed a significantly higher percentage of CD4+ T cells in lesional skin compared to unaffected skin (Figure 1A). Although there was a trend towards higher percentages of CD8+ T cells in lesional skin, the difference was not significant (Figure 1B).


CD57 expression and cytokine production by T cells in lesional and unaffected skin from patients with psoriasis.

Batista MD, Tincati C, Milush JM, Ho EL, Ndhlovu LC, York VA, Kallas EG, Kalil J, Keating SM, Norris PJ, Chang D, Unemori P, Leslie KS, Maurer T, Liao W, Nixon DF - PLoS ONE (2013)

T cell distribution in skin and PBMC of psoriasis patients.Frequency of (A) CD4+ T cells and (B) CD8+ T cells in the skin (lesional and unaffected) from psoriasis patients (n = 7). * = p<0.05.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585296&req=5

pone-0052144-g001: T cell distribution in skin and PBMC of psoriasis patients.Frequency of (A) CD4+ T cells and (B) CD8+ T cells in the skin (lesional and unaffected) from psoriasis patients (n = 7). * = p<0.05.
Mentions: We first determined whether the CD4+ or CD8+ T cell distribution was altered in unaffected skin compared to lesional skin of psoriasis patients. CD45+ leukocytes in skin samples (psoriatic lesions and non-lesional) were assessed by flow cytometry. We observed a significantly higher percentage of CD4+ T cells in lesional skin compared to unaffected skin (Figure 1A). Although there was a trend towards higher percentages of CD8+ T cells in lesional skin, the difference was not significant (Figure 1B).

Bottom Line: CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

View Article: PubMed Central - PubMed

Affiliation: Division of Experimental Medicine, Department of Medicine, University of California San Francisco, San Francisco, California, USA. maridiasbatista@gmail.com

ABSTRACT

Background: The immunopathogenic mechanisms leading to psoriasis remain unresolved. CD57 is a marker of replicative inability and immunosenescence on CD8+ T cells and the proportion of CD57 expressing CD8+ T cells is increased in a number of inflammatory conditions.

Methodology: We examined the expression of CD57 on T cells in the skin of patients affected with psoriasis, comparing lesional and unaffected skin. We also assessed functionality of the T cells by evaluating the secretion of several inflammatory cytokines (IL-17A, IFN-gamma, IL-2, IL-33, TNF-alpha, IL-21, IL-22, and IL-27), from cell-sorted purified CD4+ and CD8+ T cells isolated from lesional and unaffected skin biopsies of psoriasis patients.

Principal findings: We observed that the frequency of CD57+CD4+ and CD57+CD8+ T cells was significantly higher in unaffected skin of psoriasis patients compared to lesional skin. Sorted CD4+ T cells from psoriatic lesional skin produced higher levels of IL-17A, IL-22, and IFN-gamma compared to unaffected skin, while sorted CD8+ T cells from lesional skin produced higher levels of IL-17, IL-22, IFN-gamma, TNF-alpha, and IL-2 compared to unaffected skin.

Conclusions/significance: These findings suggest that T cells in unaffected skin from psoriasis patients exhibit a phenotype compatible with replicative inability. As they have a lower replicative capacity, CD57+ T cells are less frequent in lesional tissue due to the high cellular turnover.

Show MeSH
Related in: MedlinePlus