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Toxic effect of destruxin A on abnormal wing disc-like (SLAWD) in Spodoptera litura fabricius (Lepidoptera: Noctuidae).

Meng X, Hu J, Xu X, Wang Z, Hu Q, Jin F, Ren S - PLoS ONE (2013)

Bottom Line: To further understand the effect of DA on SLAWD expression, a functional study was carried out.The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages.Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA.

View Article: PubMed Central - PubMed

Affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, China.

ABSTRACT

Background: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out.

Principal findings: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment.

Conclusions: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression.

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Related in: MedlinePlus

SLAWD expression during the different developmental stages of S. litura.The different letters above the columns indicate significant differences in AWD gene expression during S. litura development (P<0.05).
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pone-0057213-g004: SLAWD expression during the different developmental stages of S. litura.The different letters above the columns indicate significant differences in AWD gene expression during S. litura development (P<0.05).

Mentions: To determine whether the expression profiles of SLAWD changed during S. litura development, RNA was prepared from embryos, larvae (1st to 6th stages), prepupae, pupae, and adults (male and female), and a transcriptional analysis was carried out by qRT-PCR using gene-specific primer-pairs. AWD transcripts were detected at all developmental stages (Fig. 4), indicating the consistent expression of SLAWD throughout S. litura development; however, expression was higher in the pupal and adult stages and lower in the larval and prepupal stages. These results suggested a synthetic role for AWD in the pupal stage of S. litura.


Toxic effect of destruxin A on abnormal wing disc-like (SLAWD) in Spodoptera litura fabricius (Lepidoptera: Noctuidae).

Meng X, Hu J, Xu X, Wang Z, Hu Q, Jin F, Ren S - PLoS ONE (2013)

SLAWD expression during the different developmental stages of S. litura.The different letters above the columns indicate significant differences in AWD gene expression during S. litura development (P<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585292&req=5

pone-0057213-g004: SLAWD expression during the different developmental stages of S. litura.The different letters above the columns indicate significant differences in AWD gene expression during S. litura development (P<0.05).
Mentions: To determine whether the expression profiles of SLAWD changed during S. litura development, RNA was prepared from embryos, larvae (1st to 6th stages), prepupae, pupae, and adults (male and female), and a transcriptional analysis was carried out by qRT-PCR using gene-specific primer-pairs. AWD transcripts were detected at all developmental stages (Fig. 4), indicating the consistent expression of SLAWD throughout S. litura development; however, expression was higher in the pupal and adult stages and lower in the larval and prepupal stages. These results suggested a synthetic role for AWD in the pupal stage of S. litura.

Bottom Line: To further understand the effect of DA on SLAWD expression, a functional study was carried out.The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages.Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA.

View Article: PubMed Central - PubMed

Affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, China.

ABSTRACT

Background: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out.

Principal findings: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment.

Conclusions: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression.

Show MeSH
Related in: MedlinePlus