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Toxic effect of destruxin A on abnormal wing disc-like (SLAWD) in Spodoptera litura fabricius (Lepidoptera: Noctuidae).

Meng X, Hu J, Xu X, Wang Z, Hu Q, Jin F, Ren S - PLoS ONE (2013)

Bottom Line: To further understand the effect of DA on SLAWD expression, a functional study was carried out.The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages.Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA.

View Article: PubMed Central - PubMed

Affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, China.

ABSTRACT

Background: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out.

Principal findings: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment.

Conclusions: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression.

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Multiple alignment of SLAWD and the translated amino acid sequences of AWD genes from other insects using ClustalW software.
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pone-0057213-g002: Multiple alignment of SLAWD and the translated amino acid sequences of AWD genes from other insects using ClustalW software.

Mentions: Alignment of the deduced amino acid sequence of SLAWD with sequences of AWD subunits from other insects showed that the predicted protein shares high homology with other known AWD subunits (Fig. 2). A phylogenic tree constructed using the full-length sequences of insect AWDs (Fig. 3) demonstrated high overall identity between the amino acid sequence of SLAWD and those of Choristoneura parallela abnormal wing disc-like (83%), B. mori abnormal wing disc-like (80%), and D. melanogaster abnormal wing disc-like (73%) proteins.


Toxic effect of destruxin A on abnormal wing disc-like (SLAWD) in Spodoptera litura fabricius (Lepidoptera: Noctuidae).

Meng X, Hu J, Xu X, Wang Z, Hu Q, Jin F, Ren S - PLoS ONE (2013)

Multiple alignment of SLAWD and the translated amino acid sequences of AWD genes from other insects using ClustalW software.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585292&req=5

pone-0057213-g002: Multiple alignment of SLAWD and the translated amino acid sequences of AWD genes from other insects using ClustalW software.
Mentions: Alignment of the deduced amino acid sequence of SLAWD with sequences of AWD subunits from other insects showed that the predicted protein shares high homology with other known AWD subunits (Fig. 2). A phylogenic tree constructed using the full-length sequences of insect AWDs (Fig. 3) demonstrated high overall identity between the amino acid sequence of SLAWD and those of Choristoneura parallela abnormal wing disc-like (83%), B. mori abnormal wing disc-like (80%), and D. melanogaster abnormal wing disc-like (73%) proteins.

Bottom Line: To further understand the effect of DA on SLAWD expression, a functional study was carried out.The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages.Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA.

View Article: PubMed Central - PubMed

Affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, China.

ABSTRACT

Background: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out.

Principal findings: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment.

Conclusions: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression.

Show MeSH
Related in: MedlinePlus