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Toxic effect of destruxin A on abnormal wing disc-like (SLAWD) in Spodoptera litura fabricius (Lepidoptera: Noctuidae).

Meng X, Hu J, Xu X, Wang Z, Hu Q, Jin F, Ren S - PLoS ONE (2013)

Bottom Line: To further understand the effect of DA on SLAWD expression, a functional study was carried out.The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages.Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA.

View Article: PubMed Central - PubMed

Affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, China.

ABSTRACT

Background: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out.

Principal findings: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment.

Conclusions: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression.

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SLAWD nucleotide and amino acid sequences.Boxes indicate the initiator codon and terminator codon.
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pone-0057213-g001: SLAWD nucleotide and amino acid sequences.Boxes indicate the initiator codon and terminator codon.

Mentions: The full-length SLAWD cDNA sequence was obtained and deposited in GenBank under the accession no. JF690666. In total, 764 bp of the SLAWD transcript was sequenced, We detected an open reading frame (ORF) of 537 nucleotides with the potential to encode for a protein of 178 amino acids, with a predicted molecular mass of approximately 20.10 kDa and a pI of 8.94 (Fig. 1).


Toxic effect of destruxin A on abnormal wing disc-like (SLAWD) in Spodoptera litura fabricius (Lepidoptera: Noctuidae).

Meng X, Hu J, Xu X, Wang Z, Hu Q, Jin F, Ren S - PLoS ONE (2013)

SLAWD nucleotide and amino acid sequences.Boxes indicate the initiator codon and terminator codon.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585292&req=5

pone-0057213-g001: SLAWD nucleotide and amino acid sequences.Boxes indicate the initiator codon and terminator codon.
Mentions: The full-length SLAWD cDNA sequence was obtained and deposited in GenBank under the accession no. JF690666. In total, 764 bp of the SLAWD transcript was sequenced, We detected an open reading frame (ORF) of 537 nucleotides with the potential to encode for a protein of 178 amino acids, with a predicted molecular mass of approximately 20.10 kDa and a pI of 8.94 (Fig. 1).

Bottom Line: To further understand the effect of DA on SLAWD expression, a functional study was carried out.The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages.Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA.

View Article: PubMed Central - PubMed

Affiliation: Engineering Research Center of Biological Control, Ministry of Education, South China Agricultural University, Guangzhou, China.

ABSTRACT

Background: Destruxin A (DA) is a microbial insecticide with potent bioactivity against Spodoptera litura larvae. A previous proteomic analysis of S. litura (SL-1) cells exposed to DA showed the abnormal expression of wing disc-like protein of S. litura (SLAWD). To further understand the effect of DA on SLAWD expression, a functional study was carried out.

Principal findings: The SLAWD gene (SLAWD) was cloned and an open reading frame of 537 bp encoding a polypeptide of 178 amino acids was detected. Real-time fluorescence quantitative PCR (qRT-PCR) suggested that SLAWD is expressed in all developmental stages of S. litura, but expression was highest during the pupal and adult stages. RNAi knockdown of SLAWD expression in 6th-stage larvae was achieved by the microinjection of a specific double-stranded RNA (dsRNA). The results showed a significant decrease in SLAWD mRNA expression levels between the prepupal and adult stages. Interestingly, SLAWD expression was similarly down-regulated by treating 6th-stage larvae with DA. Growth- and development-related statistics confirmed the observed abnormalities in S. litura development after either RNAi or DA treatment.

Conclusions: SLAWD appears to have a biosynthetic function in the pupal and adult stages of S. litura. The toxic effect of DA on S. litura development is due the negative effect of DA on SLAWD gene expression.

Show MeSH
Related in: MedlinePlus