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Antioxidant, antityrosinase and antitumor activity comparison: the potential utilization of fibrous root part of Bletilla striata (Thunb.) Reichb.f.

Jiang F, Li W, Huang Y, Chen Y, Jin B, Chen N, Ding Z, Ding X - PLoS ONE (2013)

Bottom Line: The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP.Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well.Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biotechnology, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

ABSTRACT
This study was carried out to evaluate the utilization probability of the fibrous root part (FRP) of Bletilla striata, which was usually discarded and harvesting pseudobulb part (PSP). The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP. Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well. Thin layer chromatography and high performance liquid chromatography analysis indicated that the chemical compositions in the two parts were similar, but the content in FRP was much higher than PSP. Meanwhile, the FRP extracts showed higher phenolic content, stronger DPPH scavenging activity, Ferric-reducing antioxidant capacity and tyrosinase inhibition activity. Sub-fraction analysis revealed that the distribution characteristic of phenolic components and other active constituents in FRP and PSP were consistent, and mainly deposited in chloroform and acetoacetate fractions. Especially, the chloroform sub-fraction (sch) of FRP showed extraordinary DPPH scavenging activity and tyrosinase inhibition activity, with IC50 0.848 mg/L and 4.3 mg/L, respectively. Besides, tyrosinase inhibition activity was even stronger than the positive compound arbutin (31.8 mg/L). Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect. Considering that an additional 0.3 kg FRP would be obtained when producing 1.0 kg PSP, our work demonstrated that FRP is very potential to be used together with PSP.

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Related in: MedlinePlus

Correlation analysis between the DPPH scavenging activity (IC50) and the phenolic content for sub-fractions of the PSP and FRP (P<0.05).
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pone-0058004-g006: Correlation analysis between the DPPH scavenging activity (IC50) and the phenolic content for sub-fractions of the PSP and FRP (P<0.05).

Mentions: The method to measure scavenging activity with DPPH as a stable free radical is widely used to evaluate antioxidant activity in food and plant extracts [25]. The scavenging abilities of extracts against DPPH radical were shown in Fig. 5A. Ethanolic extracts from both FRP and PSP had strong free radical scavenging activity. The IC50 of FRP (6.2 mg/L) was slightly lower than the positive control (2.4 mg/L) but was significantly higher than PSP (68.0 mg/L) (Fig. 5A). The IC50 values of sub-fractions were shown in Fig. 5B. As expected, the activity was tightly correlated with the content of total phenolic components in the sub-fractions of both PSP and FRP (Fig. 6). Moreover, the FRP extract showed stronger antioxidant activity than the PSP in almost all the sub-fractions except for sac. It is noticeable that sch of FRP manifested the strongest activity with IC50 0.848 mg/L, which was almost 3-fold of the positive control. While ethanolic extract showed strong antioxidant activity, the aqueous extract was absent in any activity. However, Rui et al.[26] reported that the neutral polysaccharide isolated from Bletilla striata could scavenge ·OH efficiently. The contradictory results might be due to the different free radical models established [27].


Antioxidant, antityrosinase and antitumor activity comparison: the potential utilization of fibrous root part of Bletilla striata (Thunb.) Reichb.f.

Jiang F, Li W, Huang Y, Chen Y, Jin B, Chen N, Ding Z, Ding X - PLoS ONE (2013)

Correlation analysis between the DPPH scavenging activity (IC50) and the phenolic content for sub-fractions of the PSP and FRP (P<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585276&req=5

pone-0058004-g006: Correlation analysis between the DPPH scavenging activity (IC50) and the phenolic content for sub-fractions of the PSP and FRP (P<0.05).
Mentions: The method to measure scavenging activity with DPPH as a stable free radical is widely used to evaluate antioxidant activity in food and plant extracts [25]. The scavenging abilities of extracts against DPPH radical were shown in Fig. 5A. Ethanolic extracts from both FRP and PSP had strong free radical scavenging activity. The IC50 of FRP (6.2 mg/L) was slightly lower than the positive control (2.4 mg/L) but was significantly higher than PSP (68.0 mg/L) (Fig. 5A). The IC50 values of sub-fractions were shown in Fig. 5B. As expected, the activity was tightly correlated with the content of total phenolic components in the sub-fractions of both PSP and FRP (Fig. 6). Moreover, the FRP extract showed stronger antioxidant activity than the PSP in almost all the sub-fractions except for sac. It is noticeable that sch of FRP manifested the strongest activity with IC50 0.848 mg/L, which was almost 3-fold of the positive control. While ethanolic extract showed strong antioxidant activity, the aqueous extract was absent in any activity. However, Rui et al.[26] reported that the neutral polysaccharide isolated from Bletilla striata could scavenge ·OH efficiently. The contradictory results might be due to the different free radical models established [27].

Bottom Line: The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP.Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well.Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biotechnology, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

ABSTRACT
This study was carried out to evaluate the utilization probability of the fibrous root part (FRP) of Bletilla striata, which was usually discarded and harvesting pseudobulb part (PSP). The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP. Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well. Thin layer chromatography and high performance liquid chromatography analysis indicated that the chemical compositions in the two parts were similar, but the content in FRP was much higher than PSP. Meanwhile, the FRP extracts showed higher phenolic content, stronger DPPH scavenging activity, Ferric-reducing antioxidant capacity and tyrosinase inhibition activity. Sub-fraction analysis revealed that the distribution characteristic of phenolic components and other active constituents in FRP and PSP were consistent, and mainly deposited in chloroform and acetoacetate fractions. Especially, the chloroform sub-fraction (sch) of FRP showed extraordinary DPPH scavenging activity and tyrosinase inhibition activity, with IC50 0.848 mg/L and 4.3 mg/L, respectively. Besides, tyrosinase inhibition activity was even stronger than the positive compound arbutin (31.8 mg/L). Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect. Considering that an additional 0.3 kg FRP would be obtained when producing 1.0 kg PSP, our work demonstrated that FRP is very potential to be used together with PSP.

Show MeSH
Related in: MedlinePlus