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Antioxidant, antityrosinase and antitumor activity comparison: the potential utilization of fibrous root part of Bletilla striata (Thunb.) Reichb.f.

Jiang F, Li W, Huang Y, Chen Y, Jin B, Chen N, Ding Z, Ding X - PLoS ONE (2013)

Bottom Line: The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP.Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well.Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biotechnology, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

ABSTRACT
This study was carried out to evaluate the utilization probability of the fibrous root part (FRP) of Bletilla striata, which was usually discarded and harvesting pseudobulb part (PSP). The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP. Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well. Thin layer chromatography and high performance liquid chromatography analysis indicated that the chemical compositions in the two parts were similar, but the content in FRP was much higher than PSP. Meanwhile, the FRP extracts showed higher phenolic content, stronger DPPH scavenging activity, Ferric-reducing antioxidant capacity and tyrosinase inhibition activity. Sub-fraction analysis revealed that the distribution characteristic of phenolic components and other active constituents in FRP and PSP were consistent, and mainly deposited in chloroform and acetoacetate fractions. Especially, the chloroform sub-fraction (sch) of FRP showed extraordinary DPPH scavenging activity and tyrosinase inhibition activity, with IC50 0.848 mg/L and 4.3 mg/L, respectively. Besides, tyrosinase inhibition activity was even stronger than the positive compound arbutin (31.8 mg/L). Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect. Considering that an additional 0.3 kg FRP would be obtained when producing 1.0 kg PSP, our work demonstrated that FRP is very potential to be used together with PSP.

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Related in: MedlinePlus

Analysis of the constituents of the PSP and FRP by TLC method.The plate was air dried and recorded under UV light (panel A), then developed with I2/KI under room temperature (panel B), and 5% H2SO4-ethanol in 110°C for five minutes (panel C) sequentially. The lane 1, 3 and 5 were loaded with the sub-fractions of spe, sch and sac from the FRP; lane 2, 4 and 6 were loaded with the sub-fractions of spe, sch and sac from the PSP. Red arrows indicated the components that are absent in the PSP.
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pone-0058004-g002: Analysis of the constituents of the PSP and FRP by TLC method.The plate was air dried and recorded under UV light (panel A), then developed with I2/KI under room temperature (panel B), and 5% H2SO4-ethanol in 110°C for five minutes (panel C) sequentially. The lane 1, 3 and 5 were loaded with the sub-fractions of spe, sch and sac from the FRP; lane 2, 4 and 6 were loaded with the sub-fractions of spe, sch and sac from the PSP. Red arrows indicated the components that are absent in the PSP.

Mentions: There are too many constituents in the 95% ethanol extract, and some low content compounds were difficult to be manifested on the plate. Three subfractions, spe, sch and sac, were spotted and developed for comparison. As demonstrated in Fig. 2, almost all the constituents in PSP were also detected in FRP, and even more components were discovered in FRP (Fig. 2, red arrows indicated). These results implied that FRP would exert similar therapy effect as PSP.


Antioxidant, antityrosinase and antitumor activity comparison: the potential utilization of fibrous root part of Bletilla striata (Thunb.) Reichb.f.

Jiang F, Li W, Huang Y, Chen Y, Jin B, Chen N, Ding Z, Ding X - PLoS ONE (2013)

Analysis of the constituents of the PSP and FRP by TLC method.The plate was air dried and recorded under UV light (panel A), then developed with I2/KI under room temperature (panel B), and 5% H2SO4-ethanol in 110°C for five minutes (panel C) sequentially. The lane 1, 3 and 5 were loaded with the sub-fractions of spe, sch and sac from the FRP; lane 2, 4 and 6 were loaded with the sub-fractions of spe, sch and sac from the PSP. Red arrows indicated the components that are absent in the PSP.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585276&req=5

pone-0058004-g002: Analysis of the constituents of the PSP and FRP by TLC method.The plate was air dried and recorded under UV light (panel A), then developed with I2/KI under room temperature (panel B), and 5% H2SO4-ethanol in 110°C for five minutes (panel C) sequentially. The lane 1, 3 and 5 were loaded with the sub-fractions of spe, sch and sac from the FRP; lane 2, 4 and 6 were loaded with the sub-fractions of spe, sch and sac from the PSP. Red arrows indicated the components that are absent in the PSP.
Mentions: There are too many constituents in the 95% ethanol extract, and some low content compounds were difficult to be manifested on the plate. Three subfractions, spe, sch and sac, were spotted and developed for comparison. As demonstrated in Fig. 2, almost all the constituents in PSP were also detected in FRP, and even more components were discovered in FRP (Fig. 2, red arrows indicated). These results implied that FRP would exert similar therapy effect as PSP.

Bottom Line: The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP.Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well.Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biotechnology, College of Life Science, Zhejiang Chinese Medical University, Hangzhou, China.

ABSTRACT
This study was carried out to evaluate the utilization probability of the fibrous root part (FRP) of Bletilla striata, which was usually discarded and harvesting pseudobulb part (PSP). The chemical composition, total phenolic content, DPPH radical scavenging activity, Ferric-reducing antioxidant power and tyrosinase inhibition activity were compared between FRP and PSP. Antioxidant and pro-oxidant effect as well as antitumor effect of the extract of FRP and PSP were analyzed by in vitro cell system as well. Thin layer chromatography and high performance liquid chromatography analysis indicated that the chemical compositions in the two parts were similar, but the content in FRP was much higher than PSP. Meanwhile, the FRP extracts showed higher phenolic content, stronger DPPH scavenging activity, Ferric-reducing antioxidant capacity and tyrosinase inhibition activity. Sub-fraction analysis revealed that the distribution characteristic of phenolic components and other active constituents in FRP and PSP were consistent, and mainly deposited in chloroform and acetoacetate fractions. Especially, the chloroform sub-fraction (sch) of FRP showed extraordinary DPPH scavenging activity and tyrosinase inhibition activity, with IC50 0.848 mg/L and 4.3 mg/L, respectively. Besides, tyrosinase inhibition activity was even stronger than the positive compound arbutin (31.8 mg/L). Moreover, In vitro cell system analysis confirmed that FRP extract exerts comparable activity with PSP, especially, the sub-fraction sch of FRP showed better antioxidant activity at low dosage and stronger per-oxidant activity at high dosage, and both sch of FRP and PSP can dose-dependent induce HepG2 cells apoptosis, which implied tumor therapeutic effect. Considering that an additional 0.3 kg FRP would be obtained when producing 1.0 kg PSP, our work demonstrated that FRP is very potential to be used together with PSP.

Show MeSH
Related in: MedlinePlus