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Gut microbiota patterns associated with colonization of different Clostridium difficile ribotypes.

Skraban J, Dzeroski S, Zenko B, Mongus D, Gangl S, Rupnik M - PLoS ONE (2013)

Bottom Line: Bifidobacterium longum was the single most important species associated with C. difficile negative samples.Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes.The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, University of Maribor, Maribor, Slovenia.

ABSTRACT
C. difficile infection is associated with disturbed gut microbiota and changes in relative frequencies and abundance of individual bacterial taxons have been described. In this study we have analysed bacterial, fungal and archaeal microbiota by denaturing high pressure liquid chromatography (DHPLC) and with machine learning methods in 208 faecal samples from healthy volunteers and in routine samples with requested C. difficile testing. The latter were further divided according to stool consistency, C. difficile presence or absence and C. difficile ribotype (027 or non-027). Lower microbiota diversity was a common trait of all routine samples and not necessarily connected only to C. difficile colonisation. Differences between the healthy donors and C. difficile positive routine samples were detected in bacterial, fungal and archaeal components. Bifidobacterium longum was the single most important species associated with C. difficile negative samples. However, by machine learning approaches we have identified patterns of microbiota composition predictive for C. difficile colonization. Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes. The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

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Fungal DHPLC profiles of all seven sets of samples.The samples were distributed into sets according to consistency, source (healthy/routine) and C. difficile presence. The peaks with different retention times (x - axis) represents different fungal groups.Fungal microbiota in healthy volunteers and C. difficile positive samples differs in the composition (position of the peaks) but not in diversity (number of the peaks).
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pone-0058005-g002: Fungal DHPLC profiles of all seven sets of samples.The samples were distributed into sets according to consistency, source (healthy/routine) and C. difficile presence. The peaks with different retention times (x - axis) represents different fungal groups.Fungal microbiota in healthy volunteers and C. difficile positive samples differs in the composition (position of the peaks) but not in diversity (number of the peaks).

Mentions: The fungal microbiota was more diverse (Fig 1b, Fig. 2). We have found nine different fungi altogether. The most frequent among them were Saccharomyces cerevisiae (in 57% of samples) and Candida albicans (in 27% of samples). Candida glabrata, Candida diversa, Clavispora lusitaniae, Pichia burtonii, Lemoniera sp., Eurotium sp. and Scleroderma sp. were found only sporadically.


Gut microbiota patterns associated with colonization of different Clostridium difficile ribotypes.

Skraban J, Dzeroski S, Zenko B, Mongus D, Gangl S, Rupnik M - PLoS ONE (2013)

Fungal DHPLC profiles of all seven sets of samples.The samples were distributed into sets according to consistency, source (healthy/routine) and C. difficile presence. The peaks with different retention times (x - axis) represents different fungal groups.Fungal microbiota in healthy volunteers and C. difficile positive samples differs in the composition (position of the peaks) but not in diversity (number of the peaks).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585249&req=5

pone-0058005-g002: Fungal DHPLC profiles of all seven sets of samples.The samples were distributed into sets according to consistency, source (healthy/routine) and C. difficile presence. The peaks with different retention times (x - axis) represents different fungal groups.Fungal microbiota in healthy volunteers and C. difficile positive samples differs in the composition (position of the peaks) but not in diversity (number of the peaks).
Mentions: The fungal microbiota was more diverse (Fig 1b, Fig. 2). We have found nine different fungi altogether. The most frequent among them were Saccharomyces cerevisiae (in 57% of samples) and Candida albicans (in 27% of samples). Candida glabrata, Candida diversa, Clavispora lusitaniae, Pichia burtonii, Lemoniera sp., Eurotium sp. and Scleroderma sp. were found only sporadically.

Bottom Line: Bifidobacterium longum was the single most important species associated with C. difficile negative samples.Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes.The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, University of Maribor, Maribor, Slovenia.

ABSTRACT
C. difficile infection is associated with disturbed gut microbiota and changes in relative frequencies and abundance of individual bacterial taxons have been described. In this study we have analysed bacterial, fungal and archaeal microbiota by denaturing high pressure liquid chromatography (DHPLC) and with machine learning methods in 208 faecal samples from healthy volunteers and in routine samples with requested C. difficile testing. The latter were further divided according to stool consistency, C. difficile presence or absence and C. difficile ribotype (027 or non-027). Lower microbiota diversity was a common trait of all routine samples and not necessarily connected only to C. difficile colonisation. Differences between the healthy donors and C. difficile positive routine samples were detected in bacterial, fungal and archaeal components. Bifidobacterium longum was the single most important species associated with C. difficile negative samples. However, by machine learning approaches we have identified patterns of microbiota composition predictive for C. difficile colonization. Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes. The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

Show MeSH
Related in: MedlinePlus