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Gut microbiota patterns associated with colonization of different Clostridium difficile ribotypes.

Skraban J, Dzeroski S, Zenko B, Mongus D, Gangl S, Rupnik M - PLoS ONE (2013)

Bottom Line: Bifidobacterium longum was the single most important species associated with C. difficile negative samples.Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes.The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, University of Maribor, Maribor, Slovenia.

ABSTRACT
C. difficile infection is associated with disturbed gut microbiota and changes in relative frequencies and abundance of individual bacterial taxons have been described. In this study we have analysed bacterial, fungal and archaeal microbiota by denaturing high pressure liquid chromatography (DHPLC) and with machine learning methods in 208 faecal samples from healthy volunteers and in routine samples with requested C. difficile testing. The latter were further divided according to stool consistency, C. difficile presence or absence and C. difficile ribotype (027 or non-027). Lower microbiota diversity was a common trait of all routine samples and not necessarily connected only to C. difficile colonisation. Differences between the healthy donors and C. difficile positive routine samples were detected in bacterial, fungal and archaeal components. Bifidobacterium longum was the single most important species associated with C. difficile negative samples. However, by machine learning approaches we have identified patterns of microbiota composition predictive for C. difficile colonization. Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes. The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

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Representative DHPLC profiles of faecal microbiota.The profiles show archaeal (a), fungal (b) and bacterial (c) faecal microbiota. The faecal profiles of healthy volunteers are in the upper row and the profiles from the diarrhoeal patients colonised by Clostridium difficile 027 ribotypes are in the lower row. The selected profiles show typical chromatograms of the three studied microbial groups. The chromatograms show increasing complexities from archaeal to fungal and bacterial microbiota, respectively.
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pone-0058005-g001: Representative DHPLC profiles of faecal microbiota.The profiles show archaeal (a), fungal (b) and bacterial (c) faecal microbiota. The faecal profiles of healthy volunteers are in the upper row and the profiles from the diarrhoeal patients colonised by Clostridium difficile 027 ribotypes are in the lower row. The selected profiles show typical chromatograms of the three studied microbial groups. The chromatograms show increasing complexities from archaeal to fungal and bacterial microbiota, respectively.

Mentions: The representative DHPLC profiles in Fig. 1 show different complexity of archaeal, fungal and bacterial microbiota in human faecal samples. This section will only briefly list archaeal, fungal and bacterial groups detected by the DHPLC method, while the differences in faecal microbiota between samples (healthy, C. difficile positive and negative samples) will be described in more detail in further sections.


Gut microbiota patterns associated with colonization of different Clostridium difficile ribotypes.

Skraban J, Dzeroski S, Zenko B, Mongus D, Gangl S, Rupnik M - PLoS ONE (2013)

Representative DHPLC profiles of faecal microbiota.The profiles show archaeal (a), fungal (b) and bacterial (c) faecal microbiota. The faecal profiles of healthy volunteers are in the upper row and the profiles from the diarrhoeal patients colonised by Clostridium difficile 027 ribotypes are in the lower row. The selected profiles show typical chromatograms of the three studied microbial groups. The chromatograms show increasing complexities from archaeal to fungal and bacterial microbiota, respectively.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585249&req=5

pone-0058005-g001: Representative DHPLC profiles of faecal microbiota.The profiles show archaeal (a), fungal (b) and bacterial (c) faecal microbiota. The faecal profiles of healthy volunteers are in the upper row and the profiles from the diarrhoeal patients colonised by Clostridium difficile 027 ribotypes are in the lower row. The selected profiles show typical chromatograms of the three studied microbial groups. The chromatograms show increasing complexities from archaeal to fungal and bacterial microbiota, respectively.
Mentions: The representative DHPLC profiles in Fig. 1 show different complexity of archaeal, fungal and bacterial microbiota in human faecal samples. This section will only briefly list archaeal, fungal and bacterial groups detected by the DHPLC method, while the differences in faecal microbiota between samples (healthy, C. difficile positive and negative samples) will be described in more detail in further sections.

Bottom Line: Bifidobacterium longum was the single most important species associated with C. difficile negative samples.Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes.The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, University of Maribor, Maribor, Slovenia.

ABSTRACT
C. difficile infection is associated with disturbed gut microbiota and changes in relative frequencies and abundance of individual bacterial taxons have been described. In this study we have analysed bacterial, fungal and archaeal microbiota by denaturing high pressure liquid chromatography (DHPLC) and with machine learning methods in 208 faecal samples from healthy volunteers and in routine samples with requested C. difficile testing. The latter were further divided according to stool consistency, C. difficile presence or absence and C. difficile ribotype (027 or non-027). Lower microbiota diversity was a common trait of all routine samples and not necessarily connected only to C. difficile colonisation. Differences between the healthy donors and C. difficile positive routine samples were detected in bacterial, fungal and archaeal components. Bifidobacterium longum was the single most important species associated with C. difficile negative samples. However, by machine learning approaches we have identified patterns of microbiota composition predictive for C. difficile colonization. Those patterns also differed between samples with C. difficile ribotype 027 and other C. difficile ribotypes. The results indicate that not only the presence of a single species/group is important but that certain combinations of gut microbes are associated with C. difficile carriage and that some ribotypes (027) might be associated with more disturbed microbiota than the others.

Show MeSH
Related in: MedlinePlus