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Molecular characterization of Cyclospora-like organisms from golden snub-nosed monkeys in Qinling Mountain in Shaanxi province, northwestern China.

Zhao GH, Cong MM, Bian QQ, Cheng WY, Wang RJ, Qi M, Zhang LX, Lin Q, Zhu XQ - PLoS ONE (2013)

Bottom Line: Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings.The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them.Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.

ABSTRACT
Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings. To determine the Cyclospora species in the non-human primate Rhinopithecus roxellanae, a total of 71 fecal samples from 19 endangered snub-nosed monkeys in Shaanxi province were collected and examined using Sheater's sugar flotation technique and by sequencing the fragments of 18S rDNA. Only two Cyclospora isolates from 2 golden snub-nosed monkeys (R. roxellanae) were obtained and identified between July 2011 and August of 2012. The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them. Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi. These results first showed that Cyclospora infection occurred in R. roxellanae in hot and rainy weather, which would provide useful information for further understanding the molecular epidemiology of Cyclospora spp. and the control of Cyclospora infection in non-human primates as well as in human beings.

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Related in: MedlinePlus

Phylogenetic relationships of Cyclospora isolates from golden snub-nosed monkey with Cyclospora spp. and Eimeria spp.Phylogenetic analysis was based on 18S rDNA sequences using neighbor–joining (NJ) method. The consensus tree was obtained after bootstrap analysis with 1000 replications, with values above 50% reported.
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pone-0058216-g003: Phylogenetic relationships of Cyclospora isolates from golden snub-nosed monkey with Cyclospora spp. and Eimeria spp.Phylogenetic analysis was based on 18S rDNA sequences using neighbor–joining (NJ) method. The consensus tree was obtained after bootstrap analysis with 1000 replications, with values above 50% reported.

Mentions: Then the phylogenetic relationships of some common parasites in the genera Eimeria and Cyclospora were re-constructed using NJ method based on partial 18S rDNA sequences (Figure 3). All of the Cyclospora species clustered together with high bootstrap value (95%). Within this clade, Cyclospora-like organism isolated from golden snub-nosed monkeys in the present study grouped with other monkey Cyclospora spp. and sistered to C. colobi. These results further confirmed that the Cyclospora-like organism isolated herein represents C. colobi.


Molecular characterization of Cyclospora-like organisms from golden snub-nosed monkeys in Qinling Mountain in Shaanxi province, northwestern China.

Zhao GH, Cong MM, Bian QQ, Cheng WY, Wang RJ, Qi M, Zhang LX, Lin Q, Zhu XQ - PLoS ONE (2013)

Phylogenetic relationships of Cyclospora isolates from golden snub-nosed monkey with Cyclospora spp. and Eimeria spp.Phylogenetic analysis was based on 18S rDNA sequences using neighbor–joining (NJ) method. The consensus tree was obtained after bootstrap analysis with 1000 replications, with values above 50% reported.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585237&req=5

pone-0058216-g003: Phylogenetic relationships of Cyclospora isolates from golden snub-nosed monkey with Cyclospora spp. and Eimeria spp.Phylogenetic analysis was based on 18S rDNA sequences using neighbor–joining (NJ) method. The consensus tree was obtained after bootstrap analysis with 1000 replications, with values above 50% reported.
Mentions: Then the phylogenetic relationships of some common parasites in the genera Eimeria and Cyclospora were re-constructed using NJ method based on partial 18S rDNA sequences (Figure 3). All of the Cyclospora species clustered together with high bootstrap value (95%). Within this clade, Cyclospora-like organism isolated from golden snub-nosed monkeys in the present study grouped with other monkey Cyclospora spp. and sistered to C. colobi. These results further confirmed that the Cyclospora-like organism isolated herein represents C. colobi.

Bottom Line: Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings.The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them.Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.

ABSTRACT
Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings. To determine the Cyclospora species in the non-human primate Rhinopithecus roxellanae, a total of 71 fecal samples from 19 endangered snub-nosed monkeys in Shaanxi province were collected and examined using Sheater's sugar flotation technique and by sequencing the fragments of 18S rDNA. Only two Cyclospora isolates from 2 golden snub-nosed monkeys (R. roxellanae) were obtained and identified between July 2011 and August of 2012. The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them. Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi. These results first showed that Cyclospora infection occurred in R. roxellanae in hot and rainy weather, which would provide useful information for further understanding the molecular epidemiology of Cyclospora spp. and the control of Cyclospora infection in non-human primates as well as in human beings.

Show MeSH
Related in: MedlinePlus