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Molecular characterization of Cyclospora-like organisms from golden snub-nosed monkeys in Qinling Mountain in Shaanxi province, northwestern China.

Zhao GH, Cong MM, Bian QQ, Cheng WY, Wang RJ, Qi M, Zhang LX, Lin Q, Zhu XQ - PLoS ONE (2013)

Bottom Line: Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings.The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them.Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.

ABSTRACT
Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings. To determine the Cyclospora species in the non-human primate Rhinopithecus roxellanae, a total of 71 fecal samples from 19 endangered snub-nosed monkeys in Shaanxi province were collected and examined using Sheater's sugar flotation technique and by sequencing the fragments of 18S rDNA. Only two Cyclospora isolates from 2 golden snub-nosed monkeys (R. roxellanae) were obtained and identified between July 2011 and August of 2012. The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them. Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi. These results first showed that Cyclospora infection occurred in R. roxellanae in hot and rainy weather, which would provide useful information for further understanding the molecular epidemiology of Cyclospora spp. and the control of Cyclospora infection in non-human primates as well as in human beings.

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Related in: MedlinePlus

Molecular identification of Cyclospora-like organism in the feces of the golden snub-nosed monkeys.PCR products generated with nested eimeriid-range restricted primers; 500-bp amplicons were produced from the DNA of Cyclospora-like oocysts. Lane M: DL-2000 DNA Marker, lanes 1–2: oocyst specimens from golden snub-nosed monkeys 1 and 2, respectively, and lane 3: Negative control.
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pone-0058216-g002: Molecular identification of Cyclospora-like organism in the feces of the golden snub-nosed monkeys.PCR products generated with nested eimeriid-range restricted primers; 500-bp amplicons were produced from the DNA of Cyclospora-like oocysts. Lane M: DL-2000 DNA Marker, lanes 1–2: oocyst specimens from golden snub-nosed monkeys 1 and 2, respectively, and lane 3: Negative control.

Mentions: To determine the specific identity of Cyclosopora-like oocysts, genomic DNA of each isolate was extracted and used for PCR amplification of 18S rDNA. The lengths of the PCR products from both isolates were approximately 500 bp on agarose gel electrophoresis (Figure 2). Then the purified amplicons were sequenced and intra- and inter-species sequence differences in the 18S rDNA regions were determined. The two obtained sequences were identical, with 99% similarity to corresponding sequences of Cyclospora sp. available in GenBank™ (accession numbers AF111183, AF111184, AF1111185, AF111186 and AF111187). After removing the inaccurate sequences in the both ends, 477 bp of the 18S rDNA sequence were used for pairwise comparisons. Sequence differences between the Cyclospora-like isolates obtained in the present study and C. cayetanensis, C. cercopitheci, C. colobi, C. papionis were 0.8%, 1.0%, 0.4%, and 0.8%, respectively. There results suggested that the two Cyclospora-like isolates obtained in the present study may represent C. colobi.


Molecular characterization of Cyclospora-like organisms from golden snub-nosed monkeys in Qinling Mountain in Shaanxi province, northwestern China.

Zhao GH, Cong MM, Bian QQ, Cheng WY, Wang RJ, Qi M, Zhang LX, Lin Q, Zhu XQ - PLoS ONE (2013)

Molecular identification of Cyclospora-like organism in the feces of the golden snub-nosed monkeys.PCR products generated with nested eimeriid-range restricted primers; 500-bp amplicons were produced from the DNA of Cyclospora-like oocysts. Lane M: DL-2000 DNA Marker, lanes 1–2: oocyst specimens from golden snub-nosed monkeys 1 and 2, respectively, and lane 3: Negative control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585237&req=5

pone-0058216-g002: Molecular identification of Cyclospora-like organism in the feces of the golden snub-nosed monkeys.PCR products generated with nested eimeriid-range restricted primers; 500-bp amplicons were produced from the DNA of Cyclospora-like oocysts. Lane M: DL-2000 DNA Marker, lanes 1–2: oocyst specimens from golden snub-nosed monkeys 1 and 2, respectively, and lane 3: Negative control.
Mentions: To determine the specific identity of Cyclosopora-like oocysts, genomic DNA of each isolate was extracted and used for PCR amplification of 18S rDNA. The lengths of the PCR products from both isolates were approximately 500 bp on agarose gel electrophoresis (Figure 2). Then the purified amplicons were sequenced and intra- and inter-species sequence differences in the 18S rDNA regions were determined. The two obtained sequences were identical, with 99% similarity to corresponding sequences of Cyclospora sp. available in GenBank™ (accession numbers AF111183, AF111184, AF1111185, AF111186 and AF111187). After removing the inaccurate sequences in the both ends, 477 bp of the 18S rDNA sequence were used for pairwise comparisons. Sequence differences between the Cyclospora-like isolates obtained in the present study and C. cayetanensis, C. cercopitheci, C. colobi, C. papionis were 0.8%, 1.0%, 0.4%, and 0.8%, respectively. There results suggested that the two Cyclospora-like isolates obtained in the present study may represent C. colobi.

Bottom Line: Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings.The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them.Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi.

View Article: PubMed Central - PubMed

Affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province, China.

ABSTRACT
Cyclospora spp. have been identified as one of the most important intestinal pathogens causing protracted diarrhea in animals and human beings. To determine the Cyclospora species in the non-human primate Rhinopithecus roxellanae, a total of 71 fecal samples from 19 endangered snub-nosed monkeys in Shaanxi province were collected and examined using Sheater's sugar flotation technique and by sequencing the fragments of 18S rDNA. Only two Cyclospora isolates from 2 golden snub-nosed monkeys (R. roxellanae) were obtained and identified between July 2011 and August of 2012. The sequences of the 18S rDNA for the two Cyclospora isolates were 477 bp, with no nucleotide variation between them. Phylogenetic analysis based on the 18S rDNA sequences revealed that the two Cyclospora isolates were posited into the clade Cyclospora spp. and sistered to C. colobi. These results first showed that Cyclospora infection occurred in R. roxellanae in hot and rainy weather, which would provide useful information for further understanding the molecular epidemiology of Cyclospora spp. and the control of Cyclospora infection in non-human primates as well as in human beings.

Show MeSH
Related in: MedlinePlus