Limits...
AaERF1 positively regulates the resistance to Botrytis cinerea in Artemisia annua.

Lu X, Jiang W, Zhang L, Zhang F, Zhang F, Shen Q, Wang G, Tang K - PLoS ONE (2013)

Bottom Line: The results of electrophoretic mobility shift assay (EMSA) and yeast one-hybrid experiments showed that AaERF1 was able to bind to the GCC box cis-acting element in vitro and in yeast.The down-regulated expression level of AaERF1 evidently reduced the resistance to B. cinerea in A. annua.The overall results showed that AaERF1 positively regulated the resistance to B. cinerea in A. annua.

View Article: PubMed Central - PubMed

Affiliation: Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

ABSTRACT
Plants are sessile organisms, and they can not move away under abiotic or biotic stresses. Thus plants have evolved a set of genes that response to adverse environment to modulate gene expression. In this study, we characterized and functionally studied an ERF transcription factor from Artemisia annua, AaERF1, which plays an important role in biotic stress responses. The AaERF1 promoter had been cloned and GUS staining results of AaERF1 promoter-GUS transgenic A. annua showed that AaERF1 is expressed ubiquitiously in all organs. Several putative cis-acting elements such as W-box, TGA-box and Py-rich element, which are involved in defense responsiveness, are present in the promoter. The expression of AaERF1 can be induced vigorously by methyl jasmonate as well as by ethephon and wounding, implying that AaERF1 may activate some of the defense genes via the jasmonic acid and ethylene signaling pathways of A. annua. The results of electrophoretic mobility shift assay (EMSA) and yeast one-hybrid experiments showed that AaERF1 was able to bind to the GCC box cis-acting element in vitro and in yeast. Ectopic expression of AaERF1 could enhance the expression levels of the defense marker genes PLANT DEFENSIN1.2 (PDF1.2) and BASIC CHITINASE (ChiB), and increase the resistance to Botrytis cinerea in the 35S::AaERF1 transgenic Arabidopsis. The down-regulated expression level of AaERF1 evidently reduced the resistance to B. cinerea in A. annua. The overall results showed that AaERF1 positively regulated the resistance to B. cinerea in A. annua.

Show MeSH

Related in: MedlinePlus

The 35S: AaERF1 lines show increased disease resistance.A. The numbers of control and the three independent 35S: AaERF1 transgenic Arabidopsis lines showing disease symptoms 4 d after inoculation with Botrytis cinerea. Average data with standard errors from three biological replicates are shown. B. The control and 35S: AaERF1 lines, without inoculation with Botrytis cinerea. C. The control and 35S: AaERF1, 4 d after inoculation with Botrytis cinerea, with 35S: AaERF1 plants showing reduced disease symptoms (see “Materials and Methods” for description).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3585223&req=5

pone-0057657-g006: The 35S: AaERF1 lines show increased disease resistance.A. The numbers of control and the three independent 35S: AaERF1 transgenic Arabidopsis lines showing disease symptoms 4 d after inoculation with Botrytis cinerea. Average data with standard errors from three biological replicates are shown. B. The control and 35S: AaERF1 lines, without inoculation with Botrytis cinerea. C. The control and 35S: AaERF1, 4 d after inoculation with Botrytis cinerea, with 35S: AaERF1 plants showing reduced disease symptoms (see “Materials and Methods” for description).

Mentions: The AaERF1-overexpression lines were observed following inoculation with B. cinerea. For each of the AaERF1-overexpression lines, we observed a significant reduction in the development of disease symptoms in independent inoculation experiments. Four days following inoculation with B. cinerea, 79% of the control plants showed symptoms of infection, whereas only between 32% and 42% of the leaves from AaERF1-overexpression lines were symptomatic (Figure 6A, 6C). The statistics analysis showed that the observed differences were statistically significant. The control plants turned dry and died, while most of the AaERF1-overexpression plants were growing well (Figure 6B, 6C). The results showed that the overexpression of AaERF1 could increase the disease resistance to B. cinerea in Arabidopsis.


AaERF1 positively regulates the resistance to Botrytis cinerea in Artemisia annua.

Lu X, Jiang W, Zhang L, Zhang F, Zhang F, Shen Q, Wang G, Tang K - PLoS ONE (2013)

The 35S: AaERF1 lines show increased disease resistance.A. The numbers of control and the three independent 35S: AaERF1 transgenic Arabidopsis lines showing disease symptoms 4 d after inoculation with Botrytis cinerea. Average data with standard errors from three biological replicates are shown. B. The control and 35S: AaERF1 lines, without inoculation with Botrytis cinerea. C. The control and 35S: AaERF1, 4 d after inoculation with Botrytis cinerea, with 35S: AaERF1 plants showing reduced disease symptoms (see “Materials and Methods” for description).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585223&req=5

pone-0057657-g006: The 35S: AaERF1 lines show increased disease resistance.A. The numbers of control and the three independent 35S: AaERF1 transgenic Arabidopsis lines showing disease symptoms 4 d after inoculation with Botrytis cinerea. Average data with standard errors from three biological replicates are shown. B. The control and 35S: AaERF1 lines, without inoculation with Botrytis cinerea. C. The control and 35S: AaERF1, 4 d after inoculation with Botrytis cinerea, with 35S: AaERF1 plants showing reduced disease symptoms (see “Materials and Methods” for description).
Mentions: The AaERF1-overexpression lines were observed following inoculation with B. cinerea. For each of the AaERF1-overexpression lines, we observed a significant reduction in the development of disease symptoms in independent inoculation experiments. Four days following inoculation with B. cinerea, 79% of the control plants showed symptoms of infection, whereas only between 32% and 42% of the leaves from AaERF1-overexpression lines were symptomatic (Figure 6A, 6C). The statistics analysis showed that the observed differences were statistically significant. The control plants turned dry and died, while most of the AaERF1-overexpression plants were growing well (Figure 6B, 6C). The results showed that the overexpression of AaERF1 could increase the disease resistance to B. cinerea in Arabidopsis.

Bottom Line: The results of electrophoretic mobility shift assay (EMSA) and yeast one-hybrid experiments showed that AaERF1 was able to bind to the GCC box cis-acting element in vitro and in yeast.The down-regulated expression level of AaERF1 evidently reduced the resistance to B. cinerea in A. annua.The overall results showed that AaERF1 positively regulated the resistance to B. cinerea in A. annua.

View Article: PubMed Central - PubMed

Affiliation: Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

ABSTRACT
Plants are sessile organisms, and they can not move away under abiotic or biotic stresses. Thus plants have evolved a set of genes that response to adverse environment to modulate gene expression. In this study, we characterized and functionally studied an ERF transcription factor from Artemisia annua, AaERF1, which plays an important role in biotic stress responses. The AaERF1 promoter had been cloned and GUS staining results of AaERF1 promoter-GUS transgenic A. annua showed that AaERF1 is expressed ubiquitiously in all organs. Several putative cis-acting elements such as W-box, TGA-box and Py-rich element, which are involved in defense responsiveness, are present in the promoter. The expression of AaERF1 can be induced vigorously by methyl jasmonate as well as by ethephon and wounding, implying that AaERF1 may activate some of the defense genes via the jasmonic acid and ethylene signaling pathways of A. annua. The results of electrophoretic mobility shift assay (EMSA) and yeast one-hybrid experiments showed that AaERF1 was able to bind to the GCC box cis-acting element in vitro and in yeast. Ectopic expression of AaERF1 could enhance the expression levels of the defense marker genes PLANT DEFENSIN1.2 (PDF1.2) and BASIC CHITINASE (ChiB), and increase the resistance to Botrytis cinerea in the 35S::AaERF1 transgenic Arabidopsis. The down-regulated expression level of AaERF1 evidently reduced the resistance to B. cinerea in A. annua. The overall results showed that AaERF1 positively regulated the resistance to B. cinerea in A. annua.

Show MeSH
Related in: MedlinePlus