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Functional imaging of Rel expression in inflammatory processes using bioluminescence imaging system in transgenic mice.

Yang X, Jing H, Zhao K, Sun R, Liu Z, Ying Y, Ci L, Kuang Y, Huang F, Wang Z, Fei J - PLoS ONE (2013)

Bottom Line: Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs.Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model.These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, Tongji University, Shanghai, China.

ABSTRACT
c-Rel plays important roles in many inflammatory diseases. Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs. In this paper, a transgenic mouse line, B6-Tg(c-Rel-luc)(Mlit), which incorporated the transgene firefly luciferase driven by a 14.5-kb fragment containing mouse c-Rel gene Rel promoter, was generated to monitor Rel expression in vivo. Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model. The luciferase expression in transgenic mice was comparable to the endogenous Rel expression and could be suppressed by administration of anti-inflammatory drug dexamethasone or aspirin. These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

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Inhibition of LPS induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice by dexamethasone or aspirin.(A) The LPS-induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice was inhibited both by aspirin (5 mg/kg) and dexamethasone (3 mg/kg) treatment. The mice were imaged at 0, 1, 3, 5, 24, 48 hour post injection, n = 3 (A) and the quantification of luciferase activity (intensity/sec) was presented in (B); The Rel expression in the heart and liver of B6-Tg(c-Rel-luc)8Mlit mice were measured ex vivo after LPS injection with or without aspirin or dexamethasone co-treatment, n = 3. The mRNA expression level (C) and the luciferase activity (D) were presented. n = 3. *p<0.05; **p<0.01.
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pone-0057632-g004: Inhibition of LPS induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice by dexamethasone or aspirin.(A) The LPS-induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice was inhibited both by aspirin (5 mg/kg) and dexamethasone (3 mg/kg) treatment. The mice were imaged at 0, 1, 3, 5, 24, 48 hour post injection, n = 3 (A) and the quantification of luciferase activity (intensity/sec) was presented in (B); The Rel expression in the heart and liver of B6-Tg(c-Rel-luc)8Mlit mice were measured ex vivo after LPS injection with or without aspirin or dexamethasone co-treatment, n = 3. The mRNA expression level (C) and the luciferase activity (D) were presented. n = 3. *p<0.05; **p<0.01.

Mentions: Dexamethasone and aspirin, the commonly used anti-inflammatory drugs, are reported inhibiting LPS induced sepsis [19]. In our study, the LPS-induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice could be significantly inhibited by the drug treatment (Fig. 4A). The luciferase signal were 6.8 fold as the baseline at 3 hour after LPS injection, while the values were only 3.3 fold and 4.7 fold in dexamethasone or aspirin co-treated group, respectively. The luciferase signals in the dexamethasone or aspirin co-treated group were declined to the baseline at 24 hours after LPS injection, while the signals in the control group took about 48 hours to return to the baseline (Fig. 4B). Comparing with the LPS/normal saline double treated transgenic mouse, Rel mRNA expression and luciferase activity were significantly lower in heart and liver of the LPS/dexamethasone or aspirin double treated mice at 3 hours after i.p. injection (Fig. 4C, D). These data indicated that B6-Tg(c-Rel-luc)8Mlit mice could be used as a sensitive and reliable model to evaluate anti-inflammatory drugs in vivo.


Functional imaging of Rel expression in inflammatory processes using bioluminescence imaging system in transgenic mice.

Yang X, Jing H, Zhao K, Sun R, Liu Z, Ying Y, Ci L, Kuang Y, Huang F, Wang Z, Fei J - PLoS ONE (2013)

Inhibition of LPS induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice by dexamethasone or aspirin.(A) The LPS-induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice was inhibited both by aspirin (5 mg/kg) and dexamethasone (3 mg/kg) treatment. The mice were imaged at 0, 1, 3, 5, 24, 48 hour post injection, n = 3 (A) and the quantification of luciferase activity (intensity/sec) was presented in (B); The Rel expression in the heart and liver of B6-Tg(c-Rel-luc)8Mlit mice were measured ex vivo after LPS injection with or without aspirin or dexamethasone co-treatment, n = 3. The mRNA expression level (C) and the luciferase activity (D) were presented. n = 3. *p<0.05; **p<0.01.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585201&req=5

pone-0057632-g004: Inhibition of LPS induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice by dexamethasone or aspirin.(A) The LPS-induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice was inhibited both by aspirin (5 mg/kg) and dexamethasone (3 mg/kg) treatment. The mice were imaged at 0, 1, 3, 5, 24, 48 hour post injection, n = 3 (A) and the quantification of luciferase activity (intensity/sec) was presented in (B); The Rel expression in the heart and liver of B6-Tg(c-Rel-luc)8Mlit mice were measured ex vivo after LPS injection with or without aspirin or dexamethasone co-treatment, n = 3. The mRNA expression level (C) and the luciferase activity (D) were presented. n = 3. *p<0.05; **p<0.01.
Mentions: Dexamethasone and aspirin, the commonly used anti-inflammatory drugs, are reported inhibiting LPS induced sepsis [19]. In our study, the LPS-induced luciferase activity in B6-Tg(c-Rel-luc)8Mlit mice could be significantly inhibited by the drug treatment (Fig. 4A). The luciferase signal were 6.8 fold as the baseline at 3 hour after LPS injection, while the values were only 3.3 fold and 4.7 fold in dexamethasone or aspirin co-treated group, respectively. The luciferase signals in the dexamethasone or aspirin co-treated group were declined to the baseline at 24 hours after LPS injection, while the signals in the control group took about 48 hours to return to the baseline (Fig. 4B). Comparing with the LPS/normal saline double treated transgenic mouse, Rel mRNA expression and luciferase activity were significantly lower in heart and liver of the LPS/dexamethasone or aspirin double treated mice at 3 hours after i.p. injection (Fig. 4C, D). These data indicated that B6-Tg(c-Rel-luc)8Mlit mice could be used as a sensitive and reliable model to evaluate anti-inflammatory drugs in vivo.

Bottom Line: Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs.Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model.These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, Tongji University, Shanghai, China.

ABSTRACT
c-Rel plays important roles in many inflammatory diseases. Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs. In this paper, a transgenic mouse line, B6-Tg(c-Rel-luc)(Mlit), which incorporated the transgene firefly luciferase driven by a 14.5-kb fragment containing mouse c-Rel gene Rel promoter, was generated to monitor Rel expression in vivo. Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model. The luciferase expression in transgenic mice was comparable to the endogenous Rel expression and could be suppressed by administration of anti-inflammatory drug dexamethasone or aspirin. These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

Show MeSH
Related in: MedlinePlus