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Functional imaging of Rel expression in inflammatory processes using bioluminescence imaging system in transgenic mice.

Yang X, Jing H, Zhao K, Sun R, Liu Z, Ying Y, Ci L, Kuang Y, Huang F, Wang Z, Fei J - PLoS ONE (2013)

Bottom Line: Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs.Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model.These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, Tongji University, Shanghai, China.

ABSTRACT
c-Rel plays important roles in many inflammatory diseases. Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs. In this paper, a transgenic mouse line, B6-Tg(c-Rel-luc)(Mlit), which incorporated the transgene firefly luciferase driven by a 14.5-kb fragment containing mouse c-Rel gene Rel promoter, was generated to monitor Rel expression in vivo. Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model. The luciferase expression in transgenic mice was comparable to the endogenous Rel expression and could be suppressed by administration of anti-inflammatory drug dexamethasone or aspirin. These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

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Induction of luciferase expression in B6-Tg(c-Rel-luc)8Mlit mice stimulated by LPS or Zymosan.The ventral representative images were depicted for male (A) and female (B) mice treated with LPS. (C) Male B6-Tg(c-Rel-luc)8Mlit mice were treated with zymosan (5 mg/kg). The color scale in intensity/sec was shown at the right. (D) The relative LPS induced luciferase activity. The changes of LPS induced luciferase activity were shown in fold, n = 7. (E) The changes of zymosan induced luciferase activity in fold, n = 3 in each group. *p<0.05; **p<0.01; ***p<0.001.
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pone-0057632-g002: Induction of luciferase expression in B6-Tg(c-Rel-luc)8Mlit mice stimulated by LPS or Zymosan.The ventral representative images were depicted for male (A) and female (B) mice treated with LPS. (C) Male B6-Tg(c-Rel-luc)8Mlit mice were treated with zymosan (5 mg/kg). The color scale in intensity/sec was shown at the right. (D) The relative LPS induced luciferase activity. The changes of LPS induced luciferase activity were shown in fold, n = 7. (E) The changes of zymosan induced luciferase activity in fold, n = 3 in each group. *p<0.05; **p<0.01; ***p<0.001.

Mentions: The offspring of each founder were injected intraperitoneally with LPS to test the responses of luciferase expression. Four of the nine founders showed a significant induced luciferase activity across the body after LPS i.p. injection. The line derived from the founder 8, B6-Tg(c-Rel-luc)8Mlit, with the lowest baseline of luciferase activity and highest LPS-inducible luciferase expression was chosen for the further studies. The expression of luciferase could be detected at 1 hour after LPS injection in B6-Tg(c-Rel-luc)8Mlit mice. The luminescent signal peaked at 3 h post injection in both females and males compared to the base line luminescence. In male mice, the signal peak was as much as 6.5 fold of the base line, while the value is 4.5 fold in females (n = 7 per group, p<0.002) (Fig. 2A, B). Then the signals gradually declined and returned to the base line by 48 h (n = 7 per group, p>0.05). Both male and female mice had a similar tendency of LPS-induced luminescent signal changes. The time course of luciferase activities after LPS treatment were present in Fig. 2D, E.


Functional imaging of Rel expression in inflammatory processes using bioluminescence imaging system in transgenic mice.

Yang X, Jing H, Zhao K, Sun R, Liu Z, Ying Y, Ci L, Kuang Y, Huang F, Wang Z, Fei J - PLoS ONE (2013)

Induction of luciferase expression in B6-Tg(c-Rel-luc)8Mlit mice stimulated by LPS or Zymosan.The ventral representative images were depicted for male (A) and female (B) mice treated with LPS. (C) Male B6-Tg(c-Rel-luc)8Mlit mice were treated with zymosan (5 mg/kg). The color scale in intensity/sec was shown at the right. (D) The relative LPS induced luciferase activity. The changes of LPS induced luciferase activity were shown in fold, n = 7. (E) The changes of zymosan induced luciferase activity in fold, n = 3 in each group. *p<0.05; **p<0.01; ***p<0.001.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585201&req=5

pone-0057632-g002: Induction of luciferase expression in B6-Tg(c-Rel-luc)8Mlit mice stimulated by LPS or Zymosan.The ventral representative images were depicted for male (A) and female (B) mice treated with LPS. (C) Male B6-Tg(c-Rel-luc)8Mlit mice were treated with zymosan (5 mg/kg). The color scale in intensity/sec was shown at the right. (D) The relative LPS induced luciferase activity. The changes of LPS induced luciferase activity were shown in fold, n = 7. (E) The changes of zymosan induced luciferase activity in fold, n = 3 in each group. *p<0.05; **p<0.01; ***p<0.001.
Mentions: The offspring of each founder were injected intraperitoneally with LPS to test the responses of luciferase expression. Four of the nine founders showed a significant induced luciferase activity across the body after LPS i.p. injection. The line derived from the founder 8, B6-Tg(c-Rel-luc)8Mlit, with the lowest baseline of luciferase activity and highest LPS-inducible luciferase expression was chosen for the further studies. The expression of luciferase could be detected at 1 hour after LPS injection in B6-Tg(c-Rel-luc)8Mlit mice. The luminescent signal peaked at 3 h post injection in both females and males compared to the base line luminescence. In male mice, the signal peak was as much as 6.5 fold of the base line, while the value is 4.5 fold in females (n = 7 per group, p<0.002) (Fig. 2A, B). Then the signals gradually declined and returned to the base line by 48 h (n = 7 per group, p>0.05). Both male and female mice had a similar tendency of LPS-induced luminescent signal changes. The time course of luciferase activities after LPS treatment were present in Fig. 2D, E.

Bottom Line: Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs.Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model.These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, Tongji University, Shanghai, China.

ABSTRACT
c-Rel plays important roles in many inflammatory diseases. Revealing the dynamic expression of c-Rel in disease processes in vivo is critical for understanding c-Rel functions and for developing anti-inflammatory drugs. In this paper, a transgenic mouse line, B6-Tg(c-Rel-luc)(Mlit), which incorporated the transgene firefly luciferase driven by a 14.5-kb fragment containing mouse c-Rel gene Rel promoter, was generated to monitor Rel expression in vivo. Luciferase expression could be tracked in living mice by the method of bioluminescence imaging in a variety of inflammatory processes, including LPS induced sepsis and EAE disease model. The luciferase expression in transgenic mice was comparable to the endogenous Rel expression and could be suppressed by administration of anti-inflammatory drug dexamethasone or aspirin. These results indicate that the B6-Tg(c-Rel-luc)(Mlit) mouse is a valuable animal model to study Rel expression in physiological and pathological processes, and the effects of various drug treatments in vivo.

Show MeSH
Related in: MedlinePlus