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Pollen lipidomics: lipid profiling exposes a notable diversity in 22 allergenic pollen and potential biomarkers of the allergic immune response.

Bashir ME, Lui JH, Palnivelu R, Naclerio RM, Preuss D - PLoS ONE (2013)

Bottom Line: Lipid antigens have attracted attention for their potent immunoregulatory effects.Three experiments compared pollen lipid profiles.Pollen lipids vary greatly among allergenic species and contain many molecules that have stimulatory or regulatory effects on immune responses.

View Article: PubMed Central - PubMed

Affiliation: Section of Otolaryngology-Head and Neck Surgery, Department of Surgery, Division of the Biological Sciences, The University of Chicago, Illinois, USA. mbashir@surgery.bsd.uchicago.edu

ABSTRACT

Background/aim: Pollen grains are the male gametophytes that deliver sperm cells to female gametophytes during sexual reproduction of higher plants. Pollen is a major source of aeroallergens and environmental antigens. The pollen coat harbors a plethora of lipids that are required for pollen hydration, germination, and penetration of the stigma by pollen tubes. In addition to proteins, pollen displays a wide array of lipids that interact with the human immune system. Prior searches for pollen allergens have focused on the identification of intracellular allergenic proteins, but have largely overlooked much of the extracellular pollen matrix, a region where the majority of lipid molecules reside. Lipid antigens have attracted attention for their potent immunoregulatory effects. By being in close proximity to allergenic proteins on the pollen surface when they interact with host cells, lipids could modify the antigenic properties of proteins.

Methodology/principal findings: We performed a comparative pollen lipid profiling of 22 commonly allergenic plant species by the use of gas chromatography-mass spectroscopy, followed by detailed data mining and statistical analysis. Three experiments compared pollen lipid profiles. We built a database library of the pollen lipids by matching acquired pollen-lipid mass spectra and retention times with the NIST/EPA/NIH mass-spectral library. We detected, identified, and relatively quantified more than 106 lipid molecular species including fatty acids, n-alkanes, fatty alcohols, and sterols. Pollen-derived lipids stimulation up-regulate cytokines expression of dendritic and natural killer T cells co-culture.

Conclusions/significance: Here we report on a lipidomic analysis of pollen lipids that can serve as a database for identifying potential lipid antigens and/or novel candidate molecules involved in allergy. The database provides a resource that facilitates studies on the role of lipids in the immunopathogenesis of allergy. Pollen lipids vary greatly among allergenic species and contain many molecules that have stimulatory or regulatory effects on immune responses.

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Related in: MedlinePlus

A typical total ion chromatogram (TIC) of trimethylsilyl (TMS)-derivatives of lipid molecular species obtained after separation of lipids extracted from six species of grass, weed, and tree pollen.The conditions for lipid analysis were as described in Methods.
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pone-0057566-g001: A typical total ion chromatogram (TIC) of trimethylsilyl (TMS)-derivatives of lipid molecular species obtained after separation of lipids extracted from six species of grass, weed, and tree pollen.The conditions for lipid analysis were as described in Methods.

Mentions: Figure 1 presents an example gas chromatogram of the TIC of chloroform extract prepared from the 22 pollen species. The lipid profiling of the 22 species is shown in Tables 1,2,3,4,5,6,7,8,9,10,11,12, with the complete datasets of these species available as supplemental supporting information (Table S1, Table S2, Table S3 & Table S4). The values are the mean ± SEM of three independent samples.


Pollen lipidomics: lipid profiling exposes a notable diversity in 22 allergenic pollen and potential biomarkers of the allergic immune response.

Bashir ME, Lui JH, Palnivelu R, Naclerio RM, Preuss D - PLoS ONE (2013)

A typical total ion chromatogram (TIC) of trimethylsilyl (TMS)-derivatives of lipid molecular species obtained after separation of lipids extracted from six species of grass, weed, and tree pollen.The conditions for lipid analysis were as described in Methods.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585183&req=5

pone-0057566-g001: A typical total ion chromatogram (TIC) of trimethylsilyl (TMS)-derivatives of lipid molecular species obtained after separation of lipids extracted from six species of grass, weed, and tree pollen.The conditions for lipid analysis were as described in Methods.
Mentions: Figure 1 presents an example gas chromatogram of the TIC of chloroform extract prepared from the 22 pollen species. The lipid profiling of the 22 species is shown in Tables 1,2,3,4,5,6,7,8,9,10,11,12, with the complete datasets of these species available as supplemental supporting information (Table S1, Table S2, Table S3 & Table S4). The values are the mean ± SEM of three independent samples.

Bottom Line: Lipid antigens have attracted attention for their potent immunoregulatory effects.Three experiments compared pollen lipid profiles.Pollen lipids vary greatly among allergenic species and contain many molecules that have stimulatory or regulatory effects on immune responses.

View Article: PubMed Central - PubMed

Affiliation: Section of Otolaryngology-Head and Neck Surgery, Department of Surgery, Division of the Biological Sciences, The University of Chicago, Illinois, USA. mbashir@surgery.bsd.uchicago.edu

ABSTRACT

Background/aim: Pollen grains are the male gametophytes that deliver sperm cells to female gametophytes during sexual reproduction of higher plants. Pollen is a major source of aeroallergens and environmental antigens. The pollen coat harbors a plethora of lipids that are required for pollen hydration, germination, and penetration of the stigma by pollen tubes. In addition to proteins, pollen displays a wide array of lipids that interact with the human immune system. Prior searches for pollen allergens have focused on the identification of intracellular allergenic proteins, but have largely overlooked much of the extracellular pollen matrix, a region where the majority of lipid molecules reside. Lipid antigens have attracted attention for their potent immunoregulatory effects. By being in close proximity to allergenic proteins on the pollen surface when they interact with host cells, lipids could modify the antigenic properties of proteins.

Methodology/principal findings: We performed a comparative pollen lipid profiling of 22 commonly allergenic plant species by the use of gas chromatography-mass spectroscopy, followed by detailed data mining and statistical analysis. Three experiments compared pollen lipid profiles. We built a database library of the pollen lipids by matching acquired pollen-lipid mass spectra and retention times with the NIST/EPA/NIH mass-spectral library. We detected, identified, and relatively quantified more than 106 lipid molecular species including fatty acids, n-alkanes, fatty alcohols, and sterols. Pollen-derived lipids stimulation up-regulate cytokines expression of dendritic and natural killer T cells co-culture.

Conclusions/significance: Here we report on a lipidomic analysis of pollen lipids that can serve as a database for identifying potential lipid antigens and/or novel candidate molecules involved in allergy. The database provides a resource that facilitates studies on the role of lipids in the immunopathogenesis of allergy. Pollen lipids vary greatly among allergenic species and contain many molecules that have stimulatory or regulatory effects on immune responses.

Show MeSH
Related in: MedlinePlus