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Dahl (S × R) rat congenic strain analysis confirms and defines a chromosome 17 spatial navigation quantitative trait locus to <10 Mbp.

Herrera VL, Pasion KA, Tan GA, Ruiz-Opazo N - PLoS ONE (2013)

Bottom Line: We developed two congenic strains, S.R17A and S.R17B introgressing Dahl R-chromosome 17 segments into Dahl S chromosome 17 region spanning putative Nav-5 QTL.Performance analysis of S.R17A, S.R17B and Dahl S rats in the Morris water maze (MWM) task showed a significantly decreased spatial navigation performance in S.R17B congenic rats when compared with Dahl S controls (P = 0.02).The S.R17A congenic segment did not affect MWM performance delimiting Nav-5 to the chromosome 17 65.02-74.66 Mbp region.

View Article: PubMed Central - PubMed

Affiliation: Section of Cardiovascular Medicine, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America.

ABSTRACT
A quantitative trait locus (QTL) linked with ability to find a platform in the Morris Water Maze (MWM) was located on chromosome 17 (Nav-5 QTL) using intercross between Dahl S and Dahl R rats. We developed two congenic strains, S.R17A and S.R17B introgressing Dahl R-chromosome 17 segments into Dahl S chromosome 17 region spanning putative Nav-5 QTL. Performance analysis of S.R17A, S.R17B and Dahl S rats in the Morris water maze (MWM) task showed a significantly decreased spatial navigation performance in S.R17B congenic rats when compared with Dahl S controls (P = 0.02). The S.R17A congenic segment did not affect MWM performance delimiting Nav-5 to the chromosome 17 65.02-74.66 Mbp region. Additional fine mapping is necessary to identify the specific gene variant accounting for Nav-5 effect on spatial learning and memory in Dahl rats.

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Congenic analysis of Nav-5 QTL region on chromosome 17.On the left of the figure is shown the relevant region of the physical map of rat chromosome 17. Values in parenthesis next to the marker names denote physical locations in base pairs. The mapped Nav-5 QTL region of approximately 9.64 Mbp is noted (to the right). Congenic strains are shown as solid bars (representing the Dahl R introgressed fragments) flanked by open bars (representing the putative regions of recombination).
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pone-0058280-g001: Congenic analysis of Nav-5 QTL region on chromosome 17.On the left of the figure is shown the relevant region of the physical map of rat chromosome 17. Values in parenthesis next to the marker names denote physical locations in base pairs. The mapped Nav-5 QTL region of approximately 9.64 Mbp is noted (to the right). Congenic strains are shown as solid bars (representing the Dahl R introgressed fragments) flanked by open bars (representing the putative regions of recombination).

Mentions: To substantiate the existence of Nav-5 QTL in the chromosome 17 58–80 Mbp region, we transferred two Dahl R chromosomal segments spanning the Nav-5 QTL onto the Dahl S genetic background (shown in Figure 1). We implemented a “speed congenic” strategy towards the development of highly inbred S.R17A and S.R17B (Figure 1) congenic lines. At back-cross six we established homozygous congenic lines for assessment of navigational performance on the Morris water maze (MWM) task. At BC6 S.R17A was >99.85% of Dahl S genetic background and S.R17B >99.70% of Dahl S genetic background.


Dahl (S × R) rat congenic strain analysis confirms and defines a chromosome 17 spatial navigation quantitative trait locus to <10 Mbp.

Herrera VL, Pasion KA, Tan GA, Ruiz-Opazo N - PLoS ONE (2013)

Congenic analysis of Nav-5 QTL region on chromosome 17.On the left of the figure is shown the relevant region of the physical map of rat chromosome 17. Values in parenthesis next to the marker names denote physical locations in base pairs. The mapped Nav-5 QTL region of approximately 9.64 Mbp is noted (to the right). Congenic strains are shown as solid bars (representing the Dahl R introgressed fragments) flanked by open bars (representing the putative regions of recombination).
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3585172&req=5

pone-0058280-g001: Congenic analysis of Nav-5 QTL region on chromosome 17.On the left of the figure is shown the relevant region of the physical map of rat chromosome 17. Values in parenthesis next to the marker names denote physical locations in base pairs. The mapped Nav-5 QTL region of approximately 9.64 Mbp is noted (to the right). Congenic strains are shown as solid bars (representing the Dahl R introgressed fragments) flanked by open bars (representing the putative regions of recombination).
Mentions: To substantiate the existence of Nav-5 QTL in the chromosome 17 58–80 Mbp region, we transferred two Dahl R chromosomal segments spanning the Nav-5 QTL onto the Dahl S genetic background (shown in Figure 1). We implemented a “speed congenic” strategy towards the development of highly inbred S.R17A and S.R17B (Figure 1) congenic lines. At back-cross six we established homozygous congenic lines for assessment of navigational performance on the Morris water maze (MWM) task. At BC6 S.R17A was >99.85% of Dahl S genetic background and S.R17B >99.70% of Dahl S genetic background.

Bottom Line: We developed two congenic strains, S.R17A and S.R17B introgressing Dahl R-chromosome 17 segments into Dahl S chromosome 17 region spanning putative Nav-5 QTL.Performance analysis of S.R17A, S.R17B and Dahl S rats in the Morris water maze (MWM) task showed a significantly decreased spatial navigation performance in S.R17B congenic rats when compared with Dahl S controls (P = 0.02).The S.R17A congenic segment did not affect MWM performance delimiting Nav-5 to the chromosome 17 65.02-74.66 Mbp region.

View Article: PubMed Central - PubMed

Affiliation: Section of Cardiovascular Medicine, Department of Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America.

ABSTRACT
A quantitative trait locus (QTL) linked with ability to find a platform in the Morris Water Maze (MWM) was located on chromosome 17 (Nav-5 QTL) using intercross between Dahl S and Dahl R rats. We developed two congenic strains, S.R17A and S.R17B introgressing Dahl R-chromosome 17 segments into Dahl S chromosome 17 region spanning putative Nav-5 QTL. Performance analysis of S.R17A, S.R17B and Dahl S rats in the Morris water maze (MWM) task showed a significantly decreased spatial navigation performance in S.R17B congenic rats when compared with Dahl S controls (P = 0.02). The S.R17A congenic segment did not affect MWM performance delimiting Nav-5 to the chromosome 17 65.02-74.66 Mbp region. Additional fine mapping is necessary to identify the specific gene variant accounting for Nav-5 effect on spatial learning and memory in Dahl rats.

Show MeSH