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Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts.

Lin C, Wang LH, Meng PJ, Chen CS, Tsai S - PLoS ONE (2013)

Bottom Line: Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera.On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes.Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis.

View Article: PubMed Central - PubMed

Affiliation: National Museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan.

ABSTRACT
Given the previously documented importance of lipid concentration and composition in the successful cryopreservation of gorgonian corals, these parameters were assessed in oocytes of five species of scleractinian coral; Platygyra daedalea, Echinopora gemmacea, Echinophyllia aspera, Oxypora lacera and Astreopora expansa. Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera. On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes. Triacylglycerol was not present in any species. Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis. As higher total lipid concentrations may be correlated with greater degrees of cellular membrane fluidity at lower temperatures, it stands to reason that gorgonian coral oocytes may be more likely to survive the cryopreservation process than oocytes of scleractinian corals.

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Representative images of the five target coral species used in the study.Their respective oocyte sizes (± standard error of the mean) are displayed in the adjacent column.
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pone-0057823-g003: Representative images of the five target coral species used in the study.Their respective oocyte sizes (± standard error of the mean) are displayed in the adjacent column.

Mentions: In early 2011, SCUBA divers randomly selected one representative colony of each of five species of scleractinian coral; P. daedalea, E. gemmacea, E. aspera, O. lacera and A. expansa (Fig. 3), at a depth of 3 to 5 m in Nanwan Bay, Taiwan (21°56′N, 120°44′E). Between April and May 2011, oocytes were collected in situ at night by SCUBA divers following the method developed by [4]during coral spawning events. Oocytes were immediately transported back to the laboratory and kept in an aquarium containing filtered (0.45 µm) natural seawater at 25°C for further processing. The coral collection was approved by the Kenting National Park Management Office.


Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts.

Lin C, Wang LH, Meng PJ, Chen CS, Tsai S - PLoS ONE (2013)

Representative images of the five target coral species used in the study.Their respective oocyte sizes (± standard error of the mean) are displayed in the adjacent column.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585170&req=5

pone-0057823-g003: Representative images of the five target coral species used in the study.Their respective oocyte sizes (± standard error of the mean) are displayed in the adjacent column.
Mentions: In early 2011, SCUBA divers randomly selected one representative colony of each of five species of scleractinian coral; P. daedalea, E. gemmacea, E. aspera, O. lacera and A. expansa (Fig. 3), at a depth of 3 to 5 m in Nanwan Bay, Taiwan (21°56′N, 120°44′E). Between April and May 2011, oocytes were collected in situ at night by SCUBA divers following the method developed by [4]during coral spawning events. Oocytes were immediately transported back to the laboratory and kept in an aquarium containing filtered (0.45 µm) natural seawater at 25°C for further processing. The coral collection was approved by the Kenting National Park Management Office.

Bottom Line: Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera.On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes.Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis.

View Article: PubMed Central - PubMed

Affiliation: National Museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan.

ABSTRACT
Given the previously documented importance of lipid concentration and composition in the successful cryopreservation of gorgonian corals, these parameters were assessed in oocytes of five species of scleractinian coral; Platygyra daedalea, Echinopora gemmacea, Echinophyllia aspera, Oxypora lacera and Astreopora expansa. Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera. On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes. Triacylglycerol was not present in any species. Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis. As higher total lipid concentrations may be correlated with greater degrees of cellular membrane fluidity at lower temperatures, it stands to reason that gorgonian coral oocytes may be more likely to survive the cryopreservation process than oocytes of scleractinian corals.

Show MeSH