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Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts.

Lin C, Wang LH, Meng PJ, Chen CS, Tsai S - PLoS ONE (2013)

Bottom Line: Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera.On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes.Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis.

View Article: PubMed Central - PubMed

Affiliation: National Museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan.

ABSTRACT
Given the previously documented importance of lipid concentration and composition in the successful cryopreservation of gorgonian corals, these parameters were assessed in oocytes of five species of scleractinian coral; Platygyra daedalea, Echinopora gemmacea, Echinophyllia aspera, Oxypora lacera and Astreopora expansa. Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera. On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes. Triacylglycerol was not present in any species. Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis. As higher total lipid concentrations may be correlated with greater degrees of cellular membrane fluidity at lower temperatures, it stands to reason that gorgonian coral oocytes may be more likely to survive the cryopreservation process than oocytes of scleractinian corals.

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Relative concentrations of wax esters (WE), triacylglycerol (TAGs), fatty acids (FA), phosphatidyethanolamine (PE), and phosphatidylcholine (PC) of oocytes from five coral species.
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pone-0057823-g001: Relative concentrations of wax esters (WE), triacylglycerol (TAGs), fatty acids (FA), phosphatidyethanolamine (PE), and phosphatidylcholine (PC) of oocytes from five coral species.

Mentions: Oocytes of each of the five target scleractinian coral species were an average size of 0.05±0.02 mm3. The concentrations (expressed as percentages of the total lipid concentration) of the individual lipid classes are shown in Fig. 1. The total lipid composition was similar across species, and the main oocyte lipid species were wax esters (WE), fatty acids (FA), phosphatidylethanolamines [PE], and phosphatidylcholines [PC]. The dominant lipid constituents in E. gemmacea, E. aspera and O. lacera oocytes were fatty acids (53%, 50%, and 51%, respectively). FA concentrations were lower in P. daedalea (38%) and A. expansa (19%) (Fig. 1). Higher concentrations of PE were found in P. daedalea and A. expansa (46% and 58%, respectively) than E. gemmacea, E. aspera, and O. lacera (27, 33, and 29%, respectively). The relative concentrations of WE and PE were 3–13% and 7–17%, respectively, for all species (Fig. 1). There was no triacylglycerol (TAG) detected any of five species (Fig. 1).


Lipid content and composition of oocytes from five coral species: potential implications for future cryopreservation efforts.

Lin C, Wang LH, Meng PJ, Chen CS, Tsai S - PLoS ONE (2013)

Relative concentrations of wax esters (WE), triacylglycerol (TAGs), fatty acids (FA), phosphatidyethanolamine (PE), and phosphatidylcholine (PC) of oocytes from five coral species.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585170&req=5

pone-0057823-g001: Relative concentrations of wax esters (WE), triacylglycerol (TAGs), fatty acids (FA), phosphatidyethanolamine (PE), and phosphatidylcholine (PC) of oocytes from five coral species.
Mentions: Oocytes of each of the five target scleractinian coral species were an average size of 0.05±0.02 mm3. The concentrations (expressed as percentages of the total lipid concentration) of the individual lipid classes are shown in Fig. 1. The total lipid composition was similar across species, and the main oocyte lipid species were wax esters (WE), fatty acids (FA), phosphatidylethanolamines [PE], and phosphatidylcholines [PC]. The dominant lipid constituents in E. gemmacea, E. aspera and O. lacera oocytes were fatty acids (53%, 50%, and 51%, respectively). FA concentrations were lower in P. daedalea (38%) and A. expansa (19%) (Fig. 1). Higher concentrations of PE were found in P. daedalea and A. expansa (46% and 58%, respectively) than E. gemmacea, E. aspera, and O. lacera (27, 33, and 29%, respectively). The relative concentrations of WE and PE were 3–13% and 7–17%, respectively, for all species (Fig. 1). There was no triacylglycerol (TAG) detected any of five species (Fig. 1).

Bottom Line: Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera.On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes.Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis.

View Article: PubMed Central - PubMed

Affiliation: National Museum of Marine Biology & Aquarium, Checheng, Pingtung, Taiwan.

ABSTRACT
Given the previously documented importance of lipid concentration and composition in the successful cryopreservation of gorgonian corals, these parameters were assessed in oocytes of five species of scleractinian coral; Platygyra daedalea, Echinopora gemmacea, Echinophyllia aspera, Oxypora lacera and Astreopora expansa. Wax esters, phosphatidylethanolamine, phosphatidylcholine, and fatty acids were all measured at detectable levels, and the latter were produced at significantly elevated quantities in E. gemmacea, E. aspera, and O. lacera. On the other hand, phosphatidylethanolamine, phosphatidylcholine, and wax ester were found at significantly higher concentrations in A. expansa oocytes. Triacylglycerol was not present in any species. Interestingly, the total lipid content of oocytes from all five scleractinians was significantly lower than that of oocytes of two gorgonian species, Junceella juncea and Junceella fragilis. As higher total lipid concentrations may be correlated with greater degrees of cellular membrane fluidity at lower temperatures, it stands to reason that gorgonian coral oocytes may be more likely to survive the cryopreservation process than oocytes of scleractinian corals.

Show MeSH
Related in: MedlinePlus