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Mitochondrial network genes in the skeletal muscle of amyotrophic lateral sclerosis patients.

Bernardini C, Censi F, Lattanzi W, Barba M, Calcagnini G, Giuliani A, Tasca G, Sabatelli M, Ricci E, Michetti F - PLoS ONE (2013)

Bottom Line: The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls.The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals.In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy and Cell Biology, School of Medicine, Università Cattolica del Sacro Cuore, Rome, Italy. camilla.bernardini@rm.unicatt.it

ABSTRACT
Recent evidence suggested that muscle degeneration might lead and/or contribute to neurodegeneration, thus it possibly play a key role in the etiopathogenesis and progression of amyotrophic lateral sclerosis (ALS). To test this hypothesis, this study attempted to categorize functionally relevant genes within the genome-wide expression profile of human ALS skeletal muscle, using microarray technology and gene regulatory network analysis. The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls. The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals. In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism. Overall, the analytical approach used in this study offer the possibility to observe higher levels of correlation (i.e. common expression trends) among genes, whose function seems to be aberrantly activated during the progression of muscle atrophy.

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Related in: MedlinePlus

Multidimensional scaling of expression data.MDS of the expression profiles of ALS and controls shows the correct segregation of samples (see text for details). ALS samples are represented by spheres and controls by cubes.
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pone-0057739-g002: Multidimensional scaling of expression data.MDS of the expression profiles of ALS and controls shows the correct segregation of samples (see text for details). ALS samples are represented by spheres and controls by cubes.

Mentions: The MDS analysis based on the expression level of all the 8793 probesets spotted on the array was performed in order to evaluate the segregation of the sample groups. As illustrated in figure 2, the analysis showed the efficient segregation of ALS samples from control samples.


Mitochondrial network genes in the skeletal muscle of amyotrophic lateral sclerosis patients.

Bernardini C, Censi F, Lattanzi W, Barba M, Calcagnini G, Giuliani A, Tasca G, Sabatelli M, Ricci E, Michetti F - PLoS ONE (2013)

Multidimensional scaling of expression data.MDS of the expression profiles of ALS and controls shows the correct segregation of samples (see text for details). ALS samples are represented by spheres and controls by cubes.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585165&req=5

pone-0057739-g002: Multidimensional scaling of expression data.MDS of the expression profiles of ALS and controls shows the correct segregation of samples (see text for details). ALS samples are represented by spheres and controls by cubes.
Mentions: The MDS analysis based on the expression level of all the 8793 probesets spotted on the array was performed in order to evaluate the segregation of the sample groups. As illustrated in figure 2, the analysis showed the efficient segregation of ALS samples from control samples.

Bottom Line: The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls.The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals.In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy and Cell Biology, School of Medicine, Università Cattolica del Sacro Cuore, Rome, Italy. camilla.bernardini@rm.unicatt.it

ABSTRACT
Recent evidence suggested that muscle degeneration might lead and/or contribute to neurodegeneration, thus it possibly play a key role in the etiopathogenesis and progression of amyotrophic lateral sclerosis (ALS). To test this hypothesis, this study attempted to categorize functionally relevant genes within the genome-wide expression profile of human ALS skeletal muscle, using microarray technology and gene regulatory network analysis. The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls. The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals. In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism. Overall, the analytical approach used in this study offer the possibility to observe higher levels of correlation (i.e. common expression trends) among genes, whose function seems to be aberrantly activated during the progression of muscle atrophy.

Show MeSH
Related in: MedlinePlus