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Mitochondrial network genes in the skeletal muscle of amyotrophic lateral sclerosis patients.

Bernardini C, Censi F, Lattanzi W, Barba M, Calcagnini G, Giuliani A, Tasca G, Sabatelli M, Ricci E, Michetti F - PLoS ONE (2013)

Bottom Line: The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls.The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals.In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy and Cell Biology, School of Medicine, Università Cattolica del Sacro Cuore, Rome, Italy. camilla.bernardini@rm.unicatt.it

ABSTRACT
Recent evidence suggested that muscle degeneration might lead and/or contribute to neurodegeneration, thus it possibly play a key role in the etiopathogenesis and progression of amyotrophic lateral sclerosis (ALS). To test this hypothesis, this study attempted to categorize functionally relevant genes within the genome-wide expression profile of human ALS skeletal muscle, using microarray technology and gene regulatory network analysis. The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls. The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals. In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism. Overall, the analytical approach used in this study offer the possibility to observe higher levels of correlation (i.e. common expression trends) among genes, whose function seems to be aberrantly activated during the progression of muscle atrophy.

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Related in: MedlinePlus

Experimental design.The flowchart schematizes the experimental steps of the statistical analysis of microarray data.
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Related In: Results  -  Collection


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pone-0057739-g001: Experimental design.The flowchart schematizes the experimental steps of the statistical analysis of microarray data.

Mentions: The overall data analysis process involved to distinct level of analysis, according to the flowchart depicted in figure 1.


Mitochondrial network genes in the skeletal muscle of amyotrophic lateral sclerosis patients.

Bernardini C, Censi F, Lattanzi W, Barba M, Calcagnini G, Giuliani A, Tasca G, Sabatelli M, Ricci E, Michetti F - PLoS ONE (2013)

Experimental design.The flowchart schematizes the experimental steps of the statistical analysis of microarray data.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585165&req=5

pone-0057739-g001: Experimental design.The flowchart schematizes the experimental steps of the statistical analysis of microarray data.
Mentions: The overall data analysis process involved to distinct level of analysis, according to the flowchart depicted in figure 1.

Bottom Line: The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls.The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals.In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism.

View Article: PubMed Central - PubMed

Affiliation: Institute of Anatomy and Cell Biology, School of Medicine, Università Cattolica del Sacro Cuore, Rome, Italy. camilla.bernardini@rm.unicatt.it

ABSTRACT
Recent evidence suggested that muscle degeneration might lead and/or contribute to neurodegeneration, thus it possibly play a key role in the etiopathogenesis and progression of amyotrophic lateral sclerosis (ALS). To test this hypothesis, this study attempted to categorize functionally relevant genes within the genome-wide expression profile of human ALS skeletal muscle, using microarray technology and gene regulatory network analysis. The correlation network structures significantly change between patients and controls, indicating an increased inter-gene connection in patients compared to controls. The gene network observed in the ALS group seems to reflect the perturbation of muscle homeostasis and metabolic balance occurring in affected individuals. In particular, the network observed in the ALS muscles includes genes (PRKR1A, FOXO1, TRIM32, ACTN3, among others), whose functions connect the sarcomere integrity to mitochondrial oxidative metabolism. Overall, the analytical approach used in this study offer the possibility to observe higher levels of correlation (i.e. common expression trends) among genes, whose function seems to be aberrantly activated during the progression of muscle atrophy.

Show MeSH
Related in: MedlinePlus