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flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes.

Atkinson LE, Stevenson M, McCoy CJ, Marks NJ, Fleming C, Zamanian M, Day TA, Kimber MJ, Maule AG, Mousley A - PLoS Pathog. (2013)

Bottom Line: This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate.This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R).This is the first functional characterisation of a parasitic nematode FLP-GPCR.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biosciences-Parasitology, Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.

ABSTRACT
Restrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive resource for novel control targets as it plays a critical role in sensory and motor functions. The FMRFamide-like peptides (FLPs) form the largest and most diverse family of neuropeptides in invertebrates, and are structurally conserved across nematode species, highlighting the utility of the FLPergic system as a broad-spectrum control target. flp-32 is expressed widely across nematode species. This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate. This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R). This is the first functional characterisation of a parasitic nematode FLP-GPCR.

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The rate of potato plant root infection is enhanced in Gp-flp-32-silenced Globodera pallida J2s.Infection rates of Gp-flp-32, Gp-ace (positive control siRNA), control siRNA (non-native negative control) and untreated worms identified by acid fuschin staining in the roots of potato plants challenged with nematodes post-RNAi. Gp-flp-32 siRNA treated worms show a significantly higher mean infection rate of 74% compared to control siRNA (31%) and untreated (35%) worms (P<0.001) as shown by the graph and representative light microscopy images of pink acid fuschin stained nematodes in root segments. There was no significant difference in the mean infection rate of Gp-ace siRNA, control siRNA and untreated worms.
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ppat-1003169-g006: The rate of potato plant root infection is enhanced in Gp-flp-32-silenced Globodera pallida J2s.Infection rates of Gp-flp-32, Gp-ace (positive control siRNA), control siRNA (non-native negative control) and untreated worms identified by acid fuschin staining in the roots of potato plants challenged with nematodes post-RNAi. Gp-flp-32 siRNA treated worms show a significantly higher mean infection rate of 74% compared to control siRNA (31%) and untreated (35%) worms (P<0.001) as shown by the graph and representative light microscopy images of pink acid fuschin stained nematodes in root segments. There was no significant difference in the mean infection rate of Gp-ace siRNA, control siRNA and untreated worms.

Mentions: Gp-flp-32 siRNA treated worms displayed a significantly higher mean infection rate of 74.4±5.0% (n = 4) compared to untreated (35.4±3.4%, P<0.001, q = 7.72; n = 8), non-native control siRNA (30.8±7.1%, P<0.001, q = 8.18; n = 6), and positive control siRNA (15.4±2.5%, P<0.001, q = 10.67; n = 5) treated worms (see Fig. 6). When compared, the infection rates of untreated and non-native siRNA treated controls were not significantly different (35.4±3.4% vs 30.8±7.1% infection, respectively; P>0.05, q = 1.03; Fig. 6).


flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes.

Atkinson LE, Stevenson M, McCoy CJ, Marks NJ, Fleming C, Zamanian M, Day TA, Kimber MJ, Maule AG, Mousley A - PLoS Pathog. (2013)

The rate of potato plant root infection is enhanced in Gp-flp-32-silenced Globodera pallida J2s.Infection rates of Gp-flp-32, Gp-ace (positive control siRNA), control siRNA (non-native negative control) and untreated worms identified by acid fuschin staining in the roots of potato plants challenged with nematodes post-RNAi. Gp-flp-32 siRNA treated worms show a significantly higher mean infection rate of 74% compared to control siRNA (31%) and untreated (35%) worms (P<0.001) as shown by the graph and representative light microscopy images of pink acid fuschin stained nematodes in root segments. There was no significant difference in the mean infection rate of Gp-ace siRNA, control siRNA and untreated worms.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585147&req=5

ppat-1003169-g006: The rate of potato plant root infection is enhanced in Gp-flp-32-silenced Globodera pallida J2s.Infection rates of Gp-flp-32, Gp-ace (positive control siRNA), control siRNA (non-native negative control) and untreated worms identified by acid fuschin staining in the roots of potato plants challenged with nematodes post-RNAi. Gp-flp-32 siRNA treated worms show a significantly higher mean infection rate of 74% compared to control siRNA (31%) and untreated (35%) worms (P<0.001) as shown by the graph and representative light microscopy images of pink acid fuschin stained nematodes in root segments. There was no significant difference in the mean infection rate of Gp-ace siRNA, control siRNA and untreated worms.
Mentions: Gp-flp-32 siRNA treated worms displayed a significantly higher mean infection rate of 74.4±5.0% (n = 4) compared to untreated (35.4±3.4%, P<0.001, q = 7.72; n = 8), non-native control siRNA (30.8±7.1%, P<0.001, q = 8.18; n = 6), and positive control siRNA (15.4±2.5%, P<0.001, q = 10.67; n = 5) treated worms (see Fig. 6). When compared, the infection rates of untreated and non-native siRNA treated controls were not significantly different (35.4±3.4% vs 30.8±7.1% infection, respectively; P>0.05, q = 1.03; Fig. 6).

Bottom Line: This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate.This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R).This is the first functional characterisation of a parasitic nematode FLP-GPCR.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biosciences-Parasitology, Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.

ABSTRACT
Restrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive resource for novel control targets as it plays a critical role in sensory and motor functions. The FMRFamide-like peptides (FLPs) form the largest and most diverse family of neuropeptides in invertebrates, and are structurally conserved across nematode species, highlighting the utility of the FLPergic system as a broad-spectrum control target. flp-32 is expressed widely across nematode species. This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate. This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R). This is the first functional characterisation of a parasitic nematode FLP-GPCR.

Show MeSH
Related in: MedlinePlus