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flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes.

Atkinson LE, Stevenson M, McCoy CJ, Marks NJ, Fleming C, Zamanian M, Day TA, Kimber MJ, Maule AG, Mousley A - PLoS Pathog. (2013)

Bottom Line: This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate.This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R).This is the first functional characterisation of a parasitic nematode FLP-GPCR.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biosciences-Parasitology, Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.

ABSTRACT
Restrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive resource for novel control targets as it plays a critical role in sensory and motor functions. The FMRFamide-like peptides (FLPs) form the largest and most diverse family of neuropeptides in invertebrates, and are structurally conserved across nematode species, highlighting the utility of the FLPergic system as a broad-spectrum control target. flp-32 is expressed widely across nematode species. This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate. This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R). This is the first functional characterisation of a parasitic nematode FLP-GPCR.

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Meloidogyne incognita flp-32 (Mi-flp-32) silenced nematodes exhibit accelerated migration rates.Post-RNAi Mi-flp-32 silenced worms displayed an increased speed of migration down a vertical sand column when compared to control worms. After 2 h migration 62% of Mi-flp-32 silenced worms had migrated compared to 35% and 13% of control siRNA and untreated worms, respectively (P<0.01; P<0.001); after 4 h 99% of Mi-flp-32 silenced worms had migrated compared to 73% and 58% of control siRNA and untreated worms respectively (P<0.01; P<0.001); after 6 h all Mi-flp-32 silenced worms had completed migration. There was no significant difference in the migratory ability of control siRNA and untreated worms over the 6 h migration period.
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ppat-1003169-g005: Meloidogyne incognita flp-32 (Mi-flp-32) silenced nematodes exhibit accelerated migration rates.Post-RNAi Mi-flp-32 silenced worms displayed an increased speed of migration down a vertical sand column when compared to control worms. After 2 h migration 62% of Mi-flp-32 silenced worms had migrated compared to 35% and 13% of control siRNA and untreated worms, respectively (P<0.01; P<0.001); after 4 h 99% of Mi-flp-32 silenced worms had migrated compared to 73% and 58% of control siRNA and untreated worms respectively (P<0.01; P<0.001); after 6 h all Mi-flp-32 silenced worms had completed migration. There was no significant difference in the migratory ability of control siRNA and untreated worms over the 6 h migration period.

Mentions: To ascertain if this increased rate of migration in response to flp-32 silencing is mirrored by other PPNs, the same migration experiments were performed on the pre-parasitic J2 stage of the root knot nematode Meloidogyne incognita. In these experiments worms were pre-treated with an siRNA targeting M. incognita flp-32 (Mi-flp-32; GenBank accession number CN443314; [11]) with controls as described above. flp-32 silenced pre-parasitic M. incognita migrate more rapidly than untreated or siRNA control treated worms, mirroring the phenotype of Gp-flp-32 silencing G. pallida J2s (see Fig. 5).


flp-32 Ligand/receptor silencing phenocopy faster plant pathogenic nematodes.

Atkinson LE, Stevenson M, McCoy CJ, Marks NJ, Fleming C, Zamanian M, Day TA, Kimber MJ, Maule AG, Mousley A - PLoS Pathog. (2013)

Meloidogyne incognita flp-32 (Mi-flp-32) silenced nematodes exhibit accelerated migration rates.Post-RNAi Mi-flp-32 silenced worms displayed an increased speed of migration down a vertical sand column when compared to control worms. After 2 h migration 62% of Mi-flp-32 silenced worms had migrated compared to 35% and 13% of control siRNA and untreated worms, respectively (P<0.01; P<0.001); after 4 h 99% of Mi-flp-32 silenced worms had migrated compared to 73% and 58% of control siRNA and untreated worms respectively (P<0.01; P<0.001); after 6 h all Mi-flp-32 silenced worms had completed migration. There was no significant difference in the migratory ability of control siRNA and untreated worms over the 6 h migration period.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585147&req=5

ppat-1003169-g005: Meloidogyne incognita flp-32 (Mi-flp-32) silenced nematodes exhibit accelerated migration rates.Post-RNAi Mi-flp-32 silenced worms displayed an increased speed of migration down a vertical sand column when compared to control worms. After 2 h migration 62% of Mi-flp-32 silenced worms had migrated compared to 35% and 13% of control siRNA and untreated worms, respectively (P<0.01; P<0.001); after 4 h 99% of Mi-flp-32 silenced worms had migrated compared to 73% and 58% of control siRNA and untreated worms respectively (P<0.01; P<0.001); after 6 h all Mi-flp-32 silenced worms had completed migration. There was no significant difference in the migratory ability of control siRNA and untreated worms over the 6 h migration period.
Mentions: To ascertain if this increased rate of migration in response to flp-32 silencing is mirrored by other PPNs, the same migration experiments were performed on the pre-parasitic J2 stage of the root knot nematode Meloidogyne incognita. In these experiments worms were pre-treated with an siRNA targeting M. incognita flp-32 (Mi-flp-32; GenBank accession number CN443314; [11]) with controls as described above. flp-32 silenced pre-parasitic M. incognita migrate more rapidly than untreated or siRNA control treated worms, mirroring the phenotype of Gp-flp-32 silencing G. pallida J2s (see Fig. 5).

Bottom Line: This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate.This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R).This is the first functional characterisation of a parasitic nematode FLP-GPCR.

View Article: PubMed Central - PubMed

Affiliation: Molecular Biosciences-Parasitology, Institute for Global Food Security, School of Biological Sciences, Queen's University Belfast, Belfast, United Kingdom.

ABSTRACT
Restrictions on nematicide usage underscore the need for novel control strategies for plant pathogenic nematodes such as Globodera pallida (potato cyst nematode) that impose a significant economic burden on plant cultivation activities. The nematode neuropeptide signalling system is an attractive resource for novel control targets as it plays a critical role in sensory and motor functions. The FMRFamide-like peptides (FLPs) form the largest and most diverse family of neuropeptides in invertebrates, and are structurally conserved across nematode species, highlighting the utility of the FLPergic system as a broad-spectrum control target. flp-32 is expressed widely across nematode species. This study investigates the role of flp-32 in G. pallida and shows that: (i) Gp-flp-32 encodes the peptide AMRNALVRFamide; (ii) Gp-flp-32 is expressed in the brain and ventral nerve cord of G. pallida; (iii) migration rate increases in Gp-flp-32-silenced worms; (iv) the ability of G. pallida to infect potato plant root systems is enhanced in Gp-flp-32-silenced worms; (v) a novel putative Gp-flp-32 receptor (Gp-flp-32R) is expressed in G. pallida; and, (vi) Gp-flp-32R-silenced worms also display an increase in migration rate. This work demonstrates that Gp-flp-32 plays an intrinsic role in the modulation of locomotory behaviour in G. pallida and putatively interacts with at least one novel G-protein coupled receptor (Gp-flp-32R). This is the first functional characterisation of a parasitic nematode FLP-GPCR.

Show MeSH
Related in: MedlinePlus