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γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

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The protein expressions of Wnt-1, β-catenin, c-jun, cyclin D1 and β-actin in SW620 cells.The cells treated with 15, 30, 45 and 60 µmol/L of γ-tocotrienol (A and B), or PTX at 0.2, 0.4, 0.8 and 1.2 µmol/L (C), or FH535 at 1.25, 2.5, 5 and 15 µmol/L for 24 h (D). *P<0.05, compared to the negative control group.
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pone-0057779-g009: The protein expressions of Wnt-1, β-catenin, c-jun, cyclin D1 and β-actin in SW620 cells.The cells treated with 15, 30, 45 and 60 µmol/L of γ-tocotrienol (A and B), or PTX at 0.2, 0.4, 0.8 and 1.2 µmol/L (C), or FH535 at 1.25, 2.5, 5 and 15 µmol/L for 24 h (D). *P<0.05, compared to the negative control group.

Mentions: We further examined whether protein expression associated with Wnt signaling pathway were modulated by γ-tocotrienol. As shown in Fig. 9, γ-tocotrienol significantly down-regulated the protein expression of Wnt-1 and β-catenin as well as the downstream target proteins of Wnt signaling pathway such as c-jun and cyclin D1 in a dose-dependent manner (p<0.05 or p<0.01). In addition, PTX at doses of 0.8 and 1.2 µmol/L significantly increased β-catenin protein expression and FH535 significantly decreased β-catenin protein expression in SW620 cells.


γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

The protein expressions of Wnt-1, β-catenin, c-jun, cyclin D1 and β-actin in SW620 cells.The cells treated with 15, 30, 45 and 60 µmol/L of γ-tocotrienol (A and B), or PTX at 0.2, 0.4, 0.8 and 1.2 µmol/L (C), or FH535 at 1.25, 2.5, 5 and 15 µmol/L for 24 h (D). *P<0.05, compared to the negative control group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g009: The protein expressions of Wnt-1, β-catenin, c-jun, cyclin D1 and β-actin in SW620 cells.The cells treated with 15, 30, 45 and 60 µmol/L of γ-tocotrienol (A and B), or PTX at 0.2, 0.4, 0.8 and 1.2 µmol/L (C), or FH535 at 1.25, 2.5, 5 and 15 µmol/L for 24 h (D). *P<0.05, compared to the negative control group.
Mentions: We further examined whether protein expression associated with Wnt signaling pathway were modulated by γ-tocotrienol. As shown in Fig. 9, γ-tocotrienol significantly down-regulated the protein expression of Wnt-1 and β-catenin as well as the downstream target proteins of Wnt signaling pathway such as c-jun and cyclin D1 in a dose-dependent manner (p<0.05 or p<0.01). In addition, PTX at doses of 0.8 and 1.2 µmol/L significantly increased β-catenin protein expression and FH535 significantly decreased β-catenin protein expression in SW620 cells.

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH
Related in: MedlinePlus