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γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

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The mRNA expression levels of Wnt-1, β-catenin, c-jun and cyclin D1 were detected in SW620 cells treated with different concentration of γ-tocotrienol by RT-PCR.γ-Tocotrienol significantly decreased the mRNA expression of β-catenin, c-jun and cyclin D1 in SW620 cells (P<0.05).
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pone-0057779-g008: The mRNA expression levels of Wnt-1, β-catenin, c-jun and cyclin D1 were detected in SW620 cells treated with different concentration of γ-tocotrienol by RT-PCR.γ-Tocotrienol significantly decreased the mRNA expression of β-catenin, c-jun and cyclin D1 in SW620 cells (P<0.05).

Mentions: Own to Wnt signaling pathway playing a critical role in tumor occurrence and development, the effects of γ-tocotrienol was investigated in SW620 cells by real-time PCR and western blot. The mRNA expression levels of Wnt-1, β-catenin, c-jun and cyclin D1 are shown in Fig. 8. β-Catenin, c-jun and cyclin D1 expression showed a decrease accompanying with the increasing concentrations of γ-tocotrienol. The mRNA levels of c-jun and cyclin D1 were significantly decreased in SW620 cells treated with 45 or 60 µmol/L of γ-tocotrienol when compared to the control group (P<0.05). Although the mRNA expression of Wnt-1 at dose of 15 µmol/L group was slightly higher than that in the control group, there was no differences (P>0.05). This result indicated that γ-tocotrienol had no effect on the mRNA expression of Wnt-1.


γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

The mRNA expression levels of Wnt-1, β-catenin, c-jun and cyclin D1 were detected in SW620 cells treated with different concentration of γ-tocotrienol by RT-PCR.γ-Tocotrienol significantly decreased the mRNA expression of β-catenin, c-jun and cyclin D1 in SW620 cells (P<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g008: The mRNA expression levels of Wnt-1, β-catenin, c-jun and cyclin D1 were detected in SW620 cells treated with different concentration of γ-tocotrienol by RT-PCR.γ-Tocotrienol significantly decreased the mRNA expression of β-catenin, c-jun and cyclin D1 in SW620 cells (P<0.05).
Mentions: Own to Wnt signaling pathway playing a critical role in tumor occurrence and development, the effects of γ-tocotrienol was investigated in SW620 cells by real-time PCR and western blot. The mRNA expression levels of Wnt-1, β-catenin, c-jun and cyclin D1 are shown in Fig. 8. β-Catenin, c-jun and cyclin D1 expression showed a decrease accompanying with the increasing concentrations of γ-tocotrienol. The mRNA levels of c-jun and cyclin D1 were significantly decreased in SW620 cells treated with 45 or 60 µmol/L of γ-tocotrienol when compared to the control group (P<0.05). Although the mRNA expression of Wnt-1 at dose of 15 µmol/L group was slightly higher than that in the control group, there was no differences (P>0.05). This result indicated that γ-tocotrienol had no effect on the mRNA expression of Wnt-1.

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH
Related in: MedlinePlus