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γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

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Morphological changes of paraptosis-like cell death were observed in SW620 cells by transmission electron microscopy (TEM).After treatment with 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) of γ-tocotrienol for 24 h, SW620 cells showed the swelling of mitochondria or ER (red arrowheads) and extensive cytosolic vacuolization (red arrows). The micrographs (A) provided normal cells control. The significant swellings of mitochondria or ER were observed after treated with 60 µmol/L of γ-tocotrienol for 6 h (F).
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pone-0057779-g004: Morphological changes of paraptosis-like cell death were observed in SW620 cells by transmission electron microscopy (TEM).After treatment with 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) of γ-tocotrienol for 24 h, SW620 cells showed the swelling of mitochondria or ER (red arrowheads) and extensive cytosolic vacuolization (red arrows). The micrographs (A) provided normal cells control. The significant swellings of mitochondria or ER were observed after treated with 60 µmol/L of γ-tocotrienol for 6 h (F).

Mentions: In order to detect the γ-tocotrienol-induced vacuoles in SW620 cells, the transmission electron microscopy (TEM) was emplyeed to observe the cellular ultrastructures. The results showed that swelling of the mitochondria and/or endoplasmic reticulum (ER) was detected in the cells treated with γ-tocotrienol (Fig. 4). The characteristics of classic apoptosis did not found in this study. The cells showed intact and clear organs in the control group. After treatment for 24 h, swell and formed megamitochondria were observed in the cells treated with 15 or 30 µmol/L of γ-tocotrienol groups (Fig. 4 B and C). The rates of fusion of megamitochondria and/or ER decreased and the number of vacuoles increased in the cells treated 45 and 60 µmol/L of γ-tocotrienol groups (Fig. 4D). In addition, after treatment 6 h, a plenty of large or small cytoplasmic vacuoles were observed in the cells treated with 60 µmol/L of γ-tocotrienol (Fig. 4 F). Taken together, these results demonstrate that γ-tocotrienol induces paraptosis-like cell death in SW620 cells.


γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Morphological changes of paraptosis-like cell death were observed in SW620 cells by transmission electron microscopy (TEM).After treatment with 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) of γ-tocotrienol for 24 h, SW620 cells showed the swelling of mitochondria or ER (red arrowheads) and extensive cytosolic vacuolization (red arrows). The micrographs (A) provided normal cells control. The significant swellings of mitochondria or ER were observed after treated with 60 µmol/L of γ-tocotrienol for 6 h (F).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g004: Morphological changes of paraptosis-like cell death were observed in SW620 cells by transmission electron microscopy (TEM).After treatment with 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) of γ-tocotrienol for 24 h, SW620 cells showed the swelling of mitochondria or ER (red arrowheads) and extensive cytosolic vacuolization (red arrows). The micrographs (A) provided normal cells control. The significant swellings of mitochondria or ER were observed after treated with 60 µmol/L of γ-tocotrienol for 6 h (F).
Mentions: In order to detect the γ-tocotrienol-induced vacuoles in SW620 cells, the transmission electron microscopy (TEM) was emplyeed to observe the cellular ultrastructures. The results showed that swelling of the mitochondria and/or endoplasmic reticulum (ER) was detected in the cells treated with γ-tocotrienol (Fig. 4). The characteristics of classic apoptosis did not found in this study. The cells showed intact and clear organs in the control group. After treatment for 24 h, swell and formed megamitochondria were observed in the cells treated with 15 or 30 µmol/L of γ-tocotrienol groups (Fig. 4 B and C). The rates of fusion of megamitochondria and/or ER decreased and the number of vacuoles increased in the cells treated 45 and 60 µmol/L of γ-tocotrienol groups (Fig. 4D). In addition, after treatment 6 h, a plenty of large or small cytoplasmic vacuoles were observed in the cells treated with 60 µmol/L of γ-tocotrienol (Fig. 4 F). Taken together, these results demonstrate that γ-tocotrienol induces paraptosis-like cell death in SW620 cells.

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH
Related in: MedlinePlus