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γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

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The morphological changes of cell death in HCT-8 cells.HCT-8 cells were treated with 0, 20, and 40 µmol/L of γ-tocotrienol for 24 h. The morphological changes of HCT-8 cells were detected under a light microcopy (I, Magnification: ×200). The cells were stained with DAPI (II, Magnification: ×200). Both figure showed that the cells treated with control (A), 20 (B), 40 µmol/L(C) for 24 h. Arrows indicate the vacuoles.
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pone-0057779-g003: The morphological changes of cell death in HCT-8 cells.HCT-8 cells were treated with 0, 20, and 40 µmol/L of γ-tocotrienol for 24 h. The morphological changes of HCT-8 cells were detected under a light microcopy (I, Magnification: ×200). The cells were stained with DAPI (II, Magnification: ×200). Both figure showed that the cells treated with control (A), 20 (B), 40 µmol/L(C) for 24 h. Arrows indicate the vacuoles.

Mentions: Cell death was further investigated in SW620 and HCT-8 cells in this study. The results are shown in Fig. 2 and 3. Microscopic findings showed that the cells showed a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group and the treated cells grew slowly and loosed tight contacts amongst cells (Fig. 2 and 3I). In addition, we also observed reduction of cell size, turn round of cell shape and cytoplasmic vacuolar in treated SW620 cells. The cells treated with γ-tocotrienol at dose of 60 µmol/L for 6 and 24 h showed a cytoplasmic vacuolization and the dead cells with extensive cytoplasmic vacuolization, respectively. The same phenomenon of cellular morphological changes also was observed in FH535 treated groups (Fig. 2I).


γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

The morphological changes of cell death in HCT-8 cells.HCT-8 cells were treated with 0, 20, and 40 µmol/L of γ-tocotrienol for 24 h. The morphological changes of HCT-8 cells were detected under a light microcopy (I, Magnification: ×200). The cells were stained with DAPI (II, Magnification: ×200). Both figure showed that the cells treated with control (A), 20 (B), 40 µmol/L(C) for 24 h. Arrows indicate the vacuoles.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g003: The morphological changes of cell death in HCT-8 cells.HCT-8 cells were treated with 0, 20, and 40 µmol/L of γ-tocotrienol for 24 h. The morphological changes of HCT-8 cells were detected under a light microcopy (I, Magnification: ×200). The cells were stained with DAPI (II, Magnification: ×200). Both figure showed that the cells treated with control (A), 20 (B), 40 µmol/L(C) for 24 h. Arrows indicate the vacuoles.
Mentions: Cell death was further investigated in SW620 and HCT-8 cells in this study. The results are shown in Fig. 2 and 3. Microscopic findings showed that the cells showed a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group and the treated cells grew slowly and loosed tight contacts amongst cells (Fig. 2 and 3I). In addition, we also observed reduction of cell size, turn round of cell shape and cytoplasmic vacuolar in treated SW620 cells. The cells treated with γ-tocotrienol at dose of 60 µmol/L for 6 and 24 h showed a cytoplasmic vacuolization and the dead cells with extensive cytoplasmic vacuolization, respectively. The same phenomenon of cellular morphological changes also was observed in FH535 treated groups (Fig. 2I).

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH
Related in: MedlinePlus