Limits...
γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH

Related in: MedlinePlus

The morphological changes of cell death in SW620 cells treated with γ-tocotrienol, FH535 or PTX.SW620 cells were treated with γ-tocotrienol, FH535 or PTX for 6 and 24 h. The morphological changes of SW620 cells were detected under a light microcopy (I, Magnification: ×100), a fluorescent microscope stained with DAPI (II, magnification: ×200) and AO/EB (III, magnification: ×100). The cells treated with control (A), 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) for 24 h as well as 60 µmol/L of γ-tocotrienol (F) for 6 h, or the cells treated with PTX at doses of 0(A), 0.2(B), 0.4(C), 0.8(D), 1.2(E) µmol/L, or FH535 at doses of 0(A), 0.625(B), 1.25(C), 2.5(D) µmol/L and DMSO (E) for 24 h. After treatment, the cells showed the slow growth, loosed tight contacts amongst cells and reduction of cell size, turn round of cell shape and cytoplasmic vacuolar when compare a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group. Red arrows indicate the vacuoles.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g002: The morphological changes of cell death in SW620 cells treated with γ-tocotrienol, FH535 or PTX.SW620 cells were treated with γ-tocotrienol, FH535 or PTX for 6 and 24 h. The morphological changes of SW620 cells were detected under a light microcopy (I, Magnification: ×100), a fluorescent microscope stained with DAPI (II, magnification: ×200) and AO/EB (III, magnification: ×100). The cells treated with control (A), 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) for 24 h as well as 60 µmol/L of γ-tocotrienol (F) for 6 h, or the cells treated with PTX at doses of 0(A), 0.2(B), 0.4(C), 0.8(D), 1.2(E) µmol/L, or FH535 at doses of 0(A), 0.625(B), 1.25(C), 2.5(D) µmol/L and DMSO (E) for 24 h. After treatment, the cells showed the slow growth, loosed tight contacts amongst cells and reduction of cell size, turn round of cell shape and cytoplasmic vacuolar when compare a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group. Red arrows indicate the vacuoles.

Mentions: Cell death was further investigated in SW620 and HCT-8 cells in this study. The results are shown in Fig. 2 and 3. Microscopic findings showed that the cells showed a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group and the treated cells grew slowly and loosed tight contacts amongst cells (Fig. 2 and 3I). In addition, we also observed reduction of cell size, turn round of cell shape and cytoplasmic vacuolar in treated SW620 cells. The cells treated with γ-tocotrienol at dose of 60 µmol/L for 6 and 24 h showed a cytoplasmic vacuolization and the dead cells with extensive cytoplasmic vacuolization, respectively. The same phenomenon of cellular morphological changes also was observed in FH535 treated groups (Fig. 2I).


γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

The morphological changes of cell death in SW620 cells treated with γ-tocotrienol, FH535 or PTX.SW620 cells were treated with γ-tocotrienol, FH535 or PTX for 6 and 24 h. The morphological changes of SW620 cells were detected under a light microcopy (I, Magnification: ×100), a fluorescent microscope stained with DAPI (II, magnification: ×200) and AO/EB (III, magnification: ×100). The cells treated with control (A), 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) for 24 h as well as 60 µmol/L of γ-tocotrienol (F) for 6 h, or the cells treated with PTX at doses of 0(A), 0.2(B), 0.4(C), 0.8(D), 1.2(E) µmol/L, or FH535 at doses of 0(A), 0.625(B), 1.25(C), 2.5(D) µmol/L and DMSO (E) for 24 h. After treatment, the cells showed the slow growth, loosed tight contacts amongst cells and reduction of cell size, turn round of cell shape and cytoplasmic vacuolar when compare a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group. Red arrows indicate the vacuoles.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g002: The morphological changes of cell death in SW620 cells treated with γ-tocotrienol, FH535 or PTX.SW620 cells were treated with γ-tocotrienol, FH535 or PTX for 6 and 24 h. The morphological changes of SW620 cells were detected under a light microcopy (I, Magnification: ×100), a fluorescent microscope stained with DAPI (II, magnification: ×200) and AO/EB (III, magnification: ×100). The cells treated with control (A), 15 (B), 30 (C), 45 (D) and 60 µmol/L (E) for 24 h as well as 60 µmol/L of γ-tocotrienol (F) for 6 h, or the cells treated with PTX at doses of 0(A), 0.2(B), 0.4(C), 0.8(D), 1.2(E) µmol/L, or FH535 at doses of 0(A), 0.625(B), 1.25(C), 2.5(D) µmol/L and DMSO (E) for 24 h. After treatment, the cells showed the slow growth, loosed tight contacts amongst cells and reduction of cell size, turn round of cell shape and cytoplasmic vacuolar when compare a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group. Red arrows indicate the vacuoles.
Mentions: Cell death was further investigated in SW620 and HCT-8 cells in this study. The results are shown in Fig. 2 and 3. Microscopic findings showed that the cells showed a spindly and large and grew close together, with prominent nucleoli and abundant cytoplasm in control group and the treated cells grew slowly and loosed tight contacts amongst cells (Fig. 2 and 3I). In addition, we also observed reduction of cell size, turn round of cell shape and cytoplasmic vacuolar in treated SW620 cells. The cells treated with γ-tocotrienol at dose of 60 µmol/L for 6 and 24 h showed a cytoplasmic vacuolization and the dead cells with extensive cytoplasmic vacuolization, respectively. The same phenomenon of cellular morphological changes also was observed in FH535 treated groups (Fig. 2I).

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH
Related in: MedlinePlus