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γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

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The cell viability of SW620 cells and HCT-8 cells.The panels showed γ-tocotrienol (I) and PTX (II) in SW620 cells and HCT-8 cells (III). The cells were treated with various concentrations of γ-tocotrienol or PTX for 24 h. Cell viability was determined by MTT assay. Data were expressed as means ± standard deviation (n = 6). *P<0.05, compared to the negative control group.
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pone-0057779-g001: The cell viability of SW620 cells and HCT-8 cells.The panels showed γ-tocotrienol (I) and PTX (II) in SW620 cells and HCT-8 cells (III). The cells were treated with various concentrations of γ-tocotrienol or PTX for 24 h. Cell viability was determined by MTT assay. Data were expressed as means ± standard deviation (n = 6). *P<0.05, compared to the negative control group.

Mentions: The effect of γ-tocotrienol on SW620 and HCF-8 cell proliferation was examined by MTT assay. As shown in Fig. 1, γ-tocotrienol significantly inhibited cell growth in SW620 and HCF-8 cells in a dose-dependent manner (p<0.05). The inhibitory rates of γ-tocotrienol on SW620 cells for 24 h were 77.5% at dose of 60 µmol/L when compared to the control group. The IC50 value of γ-tocotrienol was 31.4±1.51 µmol/L in SW620 cells and 32.69±1.29 µmol/L in HCT-8 cells. In the meantime, the cell viability of PTX in SW620 cells also was determined in this study. The results showed that PTX significantly inhibited cell growth in SW620 cells in a dose-dependent manner (p<0.05).


γ-Tocotrienol induces paraptosis-like cell death in human colon carcinoma SW620 cells.

Zhang JS, Li DM, Ma Y, He N, Gu Q, Wang FS, Jiang SQ, Chen BQ, Liu JR - PLoS ONE (2013)

The cell viability of SW620 cells and HCT-8 cells.The panels showed γ-tocotrienol (I) and PTX (II) in SW620 cells and HCT-8 cells (III). The cells were treated with various concentrations of γ-tocotrienol or PTX for 24 h. Cell viability was determined by MTT assay. Data were expressed as means ± standard deviation (n = 6). *P<0.05, compared to the negative control group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3585143&req=5

pone-0057779-g001: The cell viability of SW620 cells and HCT-8 cells.The panels showed γ-tocotrienol (I) and PTX (II) in SW620 cells and HCT-8 cells (III). The cells were treated with various concentrations of γ-tocotrienol or PTX for 24 h. Cell viability was determined by MTT assay. Data were expressed as means ± standard deviation (n = 6). *P<0.05, compared to the negative control group.
Mentions: The effect of γ-tocotrienol on SW620 and HCF-8 cell proliferation was examined by MTT assay. As shown in Fig. 1, γ-tocotrienol significantly inhibited cell growth in SW620 and HCF-8 cells in a dose-dependent manner (p<0.05). The inhibitory rates of γ-tocotrienol on SW620 cells for 24 h were 77.5% at dose of 60 µmol/L when compared to the control group. The IC50 value of γ-tocotrienol was 31.4±1.51 µmol/L in SW620 cells and 32.69±1.29 µmol/L in HCT-8 cells. In the meantime, the cell viability of PTX in SW620 cells also was determined in this study. The results showed that PTX significantly inhibited cell growth in SW620 cells in a dose-dependent manner (p<0.05).

Bottom Line: We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth.Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells.These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Toxicology, Tianjin Center for Disease Control and Prevention, Tianjin, People's Republic of China.

ABSTRACT
Colorectal cancer is one of the most serious illnesses among diagnosed cancer. As a new type of anti-cancer composition from tocotrienol-rich fraction of palm oil, γ-tocotrienol is widely used in anti-cancer research. The objectives of this study were to investigate the effects of γ-tocotrienol on human colon cancer SW620 and HCT-8 cells. We showed that treatment with different concentrations of γ-tocotrienol resulted in a dose dependent inhibition of cell growth. Cell death induced by γ-tocotrienol was mediated by a paraptosis-like cell death in SW620 and HCT-8 cells. Real-time RT-PCR and western blot analyses showed that γ-tocotrienol inhibited the expression level of β-catenin, cyclin D1 and c-jun. These data suggest that a paraptosis-like cell death induced by γ-tocotrienol in SW620 cells is associated with the suppression of the Wnt signaling pathway, which offers a novel tool for treating apoptosis-resistance colon cancer.

Show MeSH
Related in: MedlinePlus