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Molecular epidemiology and antibiotic susceptibility of livestock Brucella melitensis isolates from Naryn Oblast, Kyrgyzstan.

Kasymbekov J, Imanseitov J, Ballif M, Schürch N, Paniga S, Pilo P, Tonolla M, Benagli C, Akylbekova K, Jumakanova Z, Schelling E, Zinsstag J - PLoS Negl Trop Dis (2013)

Bottom Line: Multilocus variable number tandem repeat analysis showed low genetic diversity.Kyrgyz strains seem to be genetically associated with the Eastern Mediterranean group of the Brucella global phylogeny.We identified and confirmed transmission of B. melitensis to cattle and a close genetic relationship between B. melitensis strains isolated from sheep sharing the same pasture.

View Article: PubMed Central - PubMed

Affiliation: State Veterinary Department, Bishkek, Kyrgyzstan.

ABSTRACT
The incidence of human brucellosis in Kyrgyzstan has been increasing in the last years and was identified as a priority disease needing most urgent control measures in the livestock population. The latest species identification of Brucella isolates in Kyrgyzstan was carried out in the 1960s and investigated the circulation of Brucella abortus, B. melitensis, B. ovis, and B. suis. However, supporting data and documentation of that experience are lacking. Therefore, typing of Brucella spp. and identification of the most important host species are necessary for the understanding of the main transmission routes and to adopt an effective brucellosis control policy in Kyrgyzstan. Overall, 17 B. melitensis strains from aborted fetuses of sheep and cattle isolated in the province of Naryn were studied. All strains were susceptible to trimethoprim-sulfamethoxazole, gentamicin, rifampin, ofloxacin, streptomycin, doxycycline, and ciprofloxacin. Multilocus variable number tandem repeat analysis showed low genetic diversity. Kyrgyz strains seem to be genetically associated with the Eastern Mediterranean group of the Brucella global phylogeny. We identified and confirmed transmission of B. melitensis to cattle and a close genetic relationship between B. melitensis strains isolated from sheep sharing the same pasture.

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Related in: MedlinePlus

Dendrogram showing the relationship of the 15 sheep and two cattle isolates of Brucella melitensis.
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pntd-0002047-g002: Dendrogram showing the relationship of the 15 sheep and two cattle isolates of Brucella melitensis.

Mentions: Livestock systems and management of herds from which B. melitensis were isolated varied between owners. Livestock owners kept cattle and small ruminants together and practiced seasonal transhumance to high-altitude pastures. They sometimes also kept entrusted animals from several owners and traded actively animals. During the lambing seasons 2009 and 2010 in Naryn, 125 aborted fetuses (112 from sheep and 13 from cattle) were collected in the 4 villages and the city of Naryn (Figure 1). The rate of isolation for sheep was 8.9% and for cattle is 15% but the difference is not statistically significant. Urease and oxidase positive cultures were selected and 17 out of 23 isolates were confirmed B. melitensis by MALDI-ToF MS and MLVA-16 (Figure 2). The dendrogram is based on the MLVA-16 genotyping assay showing the relationship of the 15 sheep and two cattle isolates of Brucella melitensis. For each locus showing variability, the number of tandem repeats is presented. Additional information is provided on the type of sample, the local strain designation, the serial number of the animal owner and the name of the village in Naryn oblast Numbers in brackets indicate repeated isolates from the same animal. Isolates not indicated as primary were frozen prior to cultivation. Of the 17 isolates, 15 were isolated from sheep and two from cattle. All strains were susceptible to the tested antibiotics. The allelic diversity of VNTR (h) was low, with only three loci showing variation in the numbers of repeats. For locus 4 it was 0.6, for locus 16 0.16 and 0.49 for locus 30 (Table 1). All other loci did not show any variation. Eight out of 17 strains grouped into 6 different clusters. However, it has to be noted that more than one isolate was obtained from four animals. Isolates of cluster 2 were found in herds of two different owners in sheep and cattle. With regard to the geographical location, the Kyrgyz isolates are closest to strains from Kazakhstan, Israel and Iraq which are all biovar3 (Figure 3) [11].


Molecular epidemiology and antibiotic susceptibility of livestock Brucella melitensis isolates from Naryn Oblast, Kyrgyzstan.

Kasymbekov J, Imanseitov J, Ballif M, Schürch N, Paniga S, Pilo P, Tonolla M, Benagli C, Akylbekova K, Jumakanova Z, Schelling E, Zinsstag J - PLoS Negl Trop Dis (2013)

Dendrogram showing the relationship of the 15 sheep and two cattle isolates of Brucella melitensis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3584998&req=5

pntd-0002047-g002: Dendrogram showing the relationship of the 15 sheep and two cattle isolates of Brucella melitensis.
Mentions: Livestock systems and management of herds from which B. melitensis were isolated varied between owners. Livestock owners kept cattle and small ruminants together and practiced seasonal transhumance to high-altitude pastures. They sometimes also kept entrusted animals from several owners and traded actively animals. During the lambing seasons 2009 and 2010 in Naryn, 125 aborted fetuses (112 from sheep and 13 from cattle) were collected in the 4 villages and the city of Naryn (Figure 1). The rate of isolation for sheep was 8.9% and for cattle is 15% but the difference is not statistically significant. Urease and oxidase positive cultures were selected and 17 out of 23 isolates were confirmed B. melitensis by MALDI-ToF MS and MLVA-16 (Figure 2). The dendrogram is based on the MLVA-16 genotyping assay showing the relationship of the 15 sheep and two cattle isolates of Brucella melitensis. For each locus showing variability, the number of tandem repeats is presented. Additional information is provided on the type of sample, the local strain designation, the serial number of the animal owner and the name of the village in Naryn oblast Numbers in brackets indicate repeated isolates from the same animal. Isolates not indicated as primary were frozen prior to cultivation. Of the 17 isolates, 15 were isolated from sheep and two from cattle. All strains were susceptible to the tested antibiotics. The allelic diversity of VNTR (h) was low, with only three loci showing variation in the numbers of repeats. For locus 4 it was 0.6, for locus 16 0.16 and 0.49 for locus 30 (Table 1). All other loci did not show any variation. Eight out of 17 strains grouped into 6 different clusters. However, it has to be noted that more than one isolate was obtained from four animals. Isolates of cluster 2 were found in herds of two different owners in sheep and cattle. With regard to the geographical location, the Kyrgyz isolates are closest to strains from Kazakhstan, Israel and Iraq which are all biovar3 (Figure 3) [11].

Bottom Line: Multilocus variable number tandem repeat analysis showed low genetic diversity.Kyrgyz strains seem to be genetically associated with the Eastern Mediterranean group of the Brucella global phylogeny.We identified and confirmed transmission of B. melitensis to cattle and a close genetic relationship between B. melitensis strains isolated from sheep sharing the same pasture.

View Article: PubMed Central - PubMed

Affiliation: State Veterinary Department, Bishkek, Kyrgyzstan.

ABSTRACT
The incidence of human brucellosis in Kyrgyzstan has been increasing in the last years and was identified as a priority disease needing most urgent control measures in the livestock population. The latest species identification of Brucella isolates in Kyrgyzstan was carried out in the 1960s and investigated the circulation of Brucella abortus, B. melitensis, B. ovis, and B. suis. However, supporting data and documentation of that experience are lacking. Therefore, typing of Brucella spp. and identification of the most important host species are necessary for the understanding of the main transmission routes and to adopt an effective brucellosis control policy in Kyrgyzstan. Overall, 17 B. melitensis strains from aborted fetuses of sheep and cattle isolated in the province of Naryn were studied. All strains were susceptible to trimethoprim-sulfamethoxazole, gentamicin, rifampin, ofloxacin, streptomycin, doxycycline, and ciprofloxacin. Multilocus variable number tandem repeat analysis showed low genetic diversity. Kyrgyz strains seem to be genetically associated with the Eastern Mediterranean group of the Brucella global phylogeny. We identified and confirmed transmission of B. melitensis to cattle and a close genetic relationship between B. melitensis strains isolated from sheep sharing the same pasture.

Show MeSH
Related in: MedlinePlus