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Triggering receptor expressed on myeloid cells-1 (Trem-1) on blood neutrophils is associated with cytokine inducibility in human E. coli sepsis.

van Bremen T, Drömann D, Luitjens K, Dodt C, Dalhoff K, Goldmann T, Schaaf B - Diagn Pathol (2013)

Bottom Line: Bacterial sepsis induced immunsuppression via antigen hyporesponsibility increases the risk of nosokomial infections and mortality.Pattern recognition receptors (PRR) might have a central role in the pathophysiology of hyporesponsibility.We found a significant higher expression of Trem-1 and TLR-2 on monocytes and neutrophils in patients compared to healthy volunteers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Medical Clinic III, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck 23538, Germany.

ABSTRACT

Background: Bacterial sepsis induced immunsuppression via antigen hyporesponsibility increases the risk of nosokomial infections and mortality. Pattern recognition receptors (PRR) might have a central role in the pathophysiology of hyporesponsibility.

Methods: In this study we evaluated in a human E. coli sepsis cohort, the role of PRR including TLR's and Trem-1. Expression of Trem-1, TLR2, TLR4, CD14 and HLA-DR on blood monozytes and neutrophils were examined using flow cytometry from 22 patients with E. coli sepsis and 6 healthy controls. LPS and LTA stimulated TNF alpha, IL-10, IL-8 and IL-6 production was studied in a 24 h whole blood assay. Free cytokine serum concentration of TNF alpha, PCT and IP-10 were evaluated.

Results: We found a significant higher expression of Trem-1 and TLR-2 on monocytes and neutrophils in patients compared to healthy volunteers. TLR2 expression (p < 0.05) was higher and HLA-DR lower (p < 0.05) on monocytes of patients with severe sepsis compared to patients with simple sepsis. Trem-1 expression was tendentially higher (p = 0,07) on monocytes and lower on neutrophils of patients with severe sepsis. Trem-1 expression on neutrophils was associated with the IL-10 (LPS: r = 0,61, p < 0.02) and TNF-α inducibility (LPS: r = 0,78, p < 0,002). In addition Trem-1 expression on neutrophils shows a negative correlation to the serum levels of TNF alpha (r = -0,63; p < 0,005), IP-10 (r = -0,5; p < 0,035) and procalcitonin (r = -0,59; p < 0,007).

Conclusions: Patients with E. coli sepsis are characterized by an association of Trem-1 expression on blood neutrophils with cytokine inducibility. The TREM-1 pathway on neutrophils might play a role in producing an adequate inflammatory and bactericidal response in bacterial sepsis.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4441869398748313.

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A: Flow cytometry expression of TLR2, TLR4, CD14, HLA-DR, Trem-1 on monocytes. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity. B: Flow cytometry expression of TLR2, TLR4, Trem-1 on neutrophils. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity.
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Figure 1: A: Flow cytometry expression of TLR2, TLR4, CD14, HLA-DR, Trem-1 on monocytes. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity. B: Flow cytometry expression of TLR2, TLR4, Trem-1 on neutrophils. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity.

Mentions: The monocytes expression of Trem-1 and TLR2 was significant higher compared to healthy volunteers (p < 0.05). HLA-DR, CD14 and TLR4 expression showed no significance (Figure 1).


Triggering receptor expressed on myeloid cells-1 (Trem-1) on blood neutrophils is associated with cytokine inducibility in human E. coli sepsis.

van Bremen T, Drömann D, Luitjens K, Dodt C, Dalhoff K, Goldmann T, Schaaf B - Diagn Pathol (2013)

A: Flow cytometry expression of TLR2, TLR4, CD14, HLA-DR, Trem-1 on monocytes. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity. B: Flow cytometry expression of TLR2, TLR4, Trem-1 on neutrophils. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3584978&req=5

Figure 1: A: Flow cytometry expression of TLR2, TLR4, CD14, HLA-DR, Trem-1 on monocytes. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity. B: Flow cytometry expression of TLR2, TLR4, Trem-1 on neutrophils. MFI ± SD is shown for patients (P) and healthy volunteers (V); * < 0,05; MFI = mean fluorescence intensity.
Mentions: The monocytes expression of Trem-1 and TLR2 was significant higher compared to healthy volunteers (p < 0.05). HLA-DR, CD14 and TLR4 expression showed no significance (Figure 1).

Bottom Line: Bacterial sepsis induced immunsuppression via antigen hyporesponsibility increases the risk of nosokomial infections and mortality.Pattern recognition receptors (PRR) might have a central role in the pathophysiology of hyporesponsibility.We found a significant higher expression of Trem-1 and TLR-2 on monocytes and neutrophils in patients compared to healthy volunteers.

View Article: PubMed Central - HTML - PubMed

Affiliation: Medical Clinic III, University Hospital of Schleswig-Holstein, Campus Lübeck, Lübeck 23538, Germany.

ABSTRACT

Background: Bacterial sepsis induced immunsuppression via antigen hyporesponsibility increases the risk of nosokomial infections and mortality. Pattern recognition receptors (PRR) might have a central role in the pathophysiology of hyporesponsibility.

Methods: In this study we evaluated in a human E. coli sepsis cohort, the role of PRR including TLR's and Trem-1. Expression of Trem-1, TLR2, TLR4, CD14 and HLA-DR on blood monozytes and neutrophils were examined using flow cytometry from 22 patients with E. coli sepsis and 6 healthy controls. LPS and LTA stimulated TNF alpha, IL-10, IL-8 and IL-6 production was studied in a 24 h whole blood assay. Free cytokine serum concentration of TNF alpha, PCT and IP-10 were evaluated.

Results: We found a significant higher expression of Trem-1 and TLR-2 on monocytes and neutrophils in patients compared to healthy volunteers. TLR2 expression (p < 0.05) was higher and HLA-DR lower (p < 0.05) on monocytes of patients with severe sepsis compared to patients with simple sepsis. Trem-1 expression was tendentially higher (p = 0,07) on monocytes and lower on neutrophils of patients with severe sepsis. Trem-1 expression on neutrophils was associated with the IL-10 (LPS: r = 0,61, p < 0.02) and TNF-α inducibility (LPS: r = 0,78, p < 0,002). In addition Trem-1 expression on neutrophils shows a negative correlation to the serum levels of TNF alpha (r = -0,63; p < 0,005), IP-10 (r = -0,5; p < 0,035) and procalcitonin (r = -0,59; p < 0,007).

Conclusions: Patients with E. coli sepsis are characterized by an association of Trem-1 expression on blood neutrophils with cytokine inducibility. The TREM-1 pathway on neutrophils might play a role in producing an adequate inflammatory and bactericidal response in bacterial sepsis.

Virtual slides: The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/4441869398748313.

Show MeSH
Related in: MedlinePlus