Limits...
Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline.

Zhang Y, Wang X, Fang W, Cai X, Chu F, Liao X, Lu J - Bioinorg Chem Appl (2013)

Bottom Line: Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements.Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane.Complex 2 showed greater antitumor efficiency than that of complex 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.

ABSTRACT
Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (ΔΨm). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

No MeSH data available.


Related in: MedlinePlus

Distribution map of cell apoptosis. BEL-7402 cells were incubated with different concentrations of complex 1 (30, 60, and 120 μM) for 48 h, subjected to Annexin V-FITC/PI staining, analyzed by flow cytometry.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC3569890&req=5

fig9: Distribution map of cell apoptosis. BEL-7402 cells were incubated with different concentrations of complex 1 (30, 60, and 120 μM) for 48 h, subjected to Annexin V-FITC/PI staining, analyzed by flow cytometry.

Mentions: It is known that Phosphatidylserine (PS) externalization is an early feature of apoptosis and can be detected by the binding of Annexin V to PS on the cell surface [40–45], and the apoptosis assessment method by Annexin V-FITC/PI assay is well recognized and accurate. As shown in Figures 9 and 10, with increasing amounts of complexes (30 μM, 60 μM, 120 μM, resp.), the percentage of Annexin V-FITC/PI stained cells both the early and late apoptotic cells increases significantly. The results indicate that both complexes 1 and 2 induced proliferative suppression of BEL-7402 cells were via the induction of apoptosis, and the induced apoptosis effect of 2 is stronger than that of 1.


Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline.

Zhang Y, Wang X, Fang W, Cai X, Chu F, Liao X, Lu J - Bioinorg Chem Appl (2013)

Distribution map of cell apoptosis. BEL-7402 cells were incubated with different concentrations of complex 1 (30, 60, and 120 μM) for 48 h, subjected to Annexin V-FITC/PI staining, analyzed by flow cytometry.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569890&req=5

fig9: Distribution map of cell apoptosis. BEL-7402 cells were incubated with different concentrations of complex 1 (30, 60, and 120 μM) for 48 h, subjected to Annexin V-FITC/PI staining, analyzed by flow cytometry.
Mentions: It is known that Phosphatidylserine (PS) externalization is an early feature of apoptosis and can be detected by the binding of Annexin V to PS on the cell surface [40–45], and the apoptosis assessment method by Annexin V-FITC/PI assay is well recognized and accurate. As shown in Figures 9 and 10, with increasing amounts of complexes (30 μM, 60 μM, 120 μM, resp.), the percentage of Annexin V-FITC/PI stained cells both the early and late apoptotic cells increases significantly. The results indicate that both complexes 1 and 2 induced proliferative suppression of BEL-7402 cells were via the induction of apoptosis, and the induced apoptosis effect of 2 is stronger than that of 1.

Bottom Line: Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements.Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane.Complex 2 showed greater antitumor efficiency than that of complex 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.

ABSTRACT
Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (ΔΨm). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

No MeSH data available.


Related in: MedlinePlus