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Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline.

Zhang Y, Wang X, Fang W, Cai X, Chu F, Liao X, Lu J - Bioinorg Chem Appl (2013)

Bottom Line: Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements.Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane.Complex 2 showed greater antitumor efficiency than that of complex 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.

ABSTRACT
Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (ΔΨm). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

No MeSH data available.


Related in: MedlinePlus

Effects of complex 2 on the morphology of Bel-7402 cells were assayed by Hoechst 33342 staining. After treatment with complex 2 for 48 h, apoptotic cells were detected by Hoechst 33342 staining and examined by fluorescence microscopy (original magnification 400x).
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fig8: Effects of complex 2 on the morphology of Bel-7402 cells were assayed by Hoechst 33342 staining. After treatment with complex 2 for 48 h, apoptotic cells were detected by Hoechst 33342 staining and examined by fluorescence microscopy (original magnification 400x).

Mentions: As shown in Figures 7 and 8, compared with the control, the BEL-7402 cells exhibited typical apoptotic features after treated with complexes 1 and 2 for 48 h including cellular morphological change, the apoptotic bodies, condensation of chromatin (brightly stained), and the apoptotic cells significantly increased in a dose-dependent manner with increasing the concentrations (30, 60, and 120 μM, resp.) of 1 and 2.


Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline.

Zhang Y, Wang X, Fang W, Cai X, Chu F, Liao X, Lu J - Bioinorg Chem Appl (2013)

Effects of complex 2 on the morphology of Bel-7402 cells were assayed by Hoechst 33342 staining. After treatment with complex 2 for 48 h, apoptotic cells were detected by Hoechst 33342 staining and examined by fluorescence microscopy (original magnification 400x).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569890&req=5

fig8: Effects of complex 2 on the morphology of Bel-7402 cells were assayed by Hoechst 33342 staining. After treatment with complex 2 for 48 h, apoptotic cells were detected by Hoechst 33342 staining and examined by fluorescence microscopy (original magnification 400x).
Mentions: As shown in Figures 7 and 8, compared with the control, the BEL-7402 cells exhibited typical apoptotic features after treated with complexes 1 and 2 for 48 h including cellular morphological change, the apoptotic bodies, condensation of chromatin (brightly stained), and the apoptotic cells significantly increased in a dose-dependent manner with increasing the concentrations (30, 60, and 120 μM, resp.) of 1 and 2.

Bottom Line: Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements.Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane.Complex 2 showed greater antitumor efficiency than that of complex 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.

ABSTRACT
Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (ΔΨm). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

No MeSH data available.


Related in: MedlinePlus