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Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline.

Zhang Y, Wang X, Fang W, Cai X, Chu F, Liao X, Lu J - Bioinorg Chem Appl (2013)

Bottom Line: Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements.Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane.Complex 2 showed greater antitumor efficiency than that of complex 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.

ABSTRACT
Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (ΔΨm). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

No MeSH data available.


Related in: MedlinePlus

Antiproliferative activity of complexes 1 (a) and 2 (b) detected by MTT assay after 24, 48, and 72 h of treatment on HepG2 cells.
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fig4: Antiproliferative activity of complexes 1 (a) and 2 (b) detected by MTT assay after 24, 48, and 72 h of treatment on HepG2 cells.

Mentions: Complexes 1 and 2 were evaluated for their ability to inhibit the growth of BEL-7402, HUH-7, and HepG2 human hepatoma cell lines using MTT assay. The inhibition was expressed as cell viability relative to control without 1 and 2 treatments. In the present study, BEL-7402, HUH-7, and HepG2 human hepatoma cells were used which have been recently characterized as a suitable model for in vitro assessment of hepatoma toxicity [32, 33]. And 5-Fluorouracil (5-FU, 30 μM) was used as a positive control, which has been used extensively as an efficient anticancer drug in clinical trials [32–34]. After treated for 24 h, 48 h, and 72 h on the selected three cell lines, the cells viability is showed in Figures 2, 3, and 4 and the IC50 values is summarized in Table 2.


Synthesis and In Vitro Antitumor Activity of Two Mixed-Ligand Oxovanadium(IV) Complexes of Schiff Base and Phenanthroline.

Zhang Y, Wang X, Fang W, Cai X, Chu F, Liao X, Lu J - Bioinorg Chem Appl (2013)

Antiproliferative activity of complexes 1 (a) and 2 (b) detected by MTT assay after 24, 48, and 72 h of treatment on HepG2 cells.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569890&req=5

fig4: Antiproliferative activity of complexes 1 (a) and 2 (b) detected by MTT assay after 24, 48, and 72 h of treatment on HepG2 cells.
Mentions: Complexes 1 and 2 were evaluated for their ability to inhibit the growth of BEL-7402, HUH-7, and HepG2 human hepatoma cell lines using MTT assay. The inhibition was expressed as cell viability relative to control without 1 and 2 treatments. In the present study, BEL-7402, HUH-7, and HepG2 human hepatoma cells were used which have been recently characterized as a suitable model for in vitro assessment of hepatoma toxicity [32, 33]. And 5-Fluorouracil (5-FU, 30 μM) was used as a positive control, which has been used extensively as an efficient anticancer drug in clinical trials [32–34]. After treated for 24 h, 48 h, and 72 h on the selected three cell lines, the cells viability is showed in Figures 2, 3, and 4 and the IC50 values is summarized in Table 2.

Bottom Line: Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements.Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane.Complex 2 showed greater antitumor efficiency than that of complex 1.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.

ABSTRACT
Two oxovanadium(IV) complexes of [VO(msatsc)(phen)], (1) (msatsc = methoxylsalicylaldehyde thiosemicarbazone, phen = phenanthroline) and its novel derivative [VO (4-chlorosatsc)(phen)], (2) (4-chlorosatsc = 4-chlorosalicylaldehyde thiosemicarbazone), have been synthesized and characterized by elemental analysis, IR, ES-MS, (1)H NMR, and magnetic susceptibility measurements. Their antitumor effects on BEL-7402, HUH-7, and HepG2 cells were studied by MTT assay. The antitumor biological mechanism of these two complexes was studied in BEL-7402 cells by cell cycle analysis, Hoechst 33342 staining, Annexin V-FITC/PI assay, and detection of mitochondrial membrane potential (ΔΨm). The results showed that the growth of cancer cells was inhibited significantly, and complexes 1 and 2 mainly caused in BEL-7402 cells G0/G1 cell cycle arrest and induced apoptosis. Both 1 and 2 decreased significantly the ΔΨm, causing the depolarization of the mitochondrial membrane. Complex 2 showed greater antitumor efficiency than that of complex 1.

No MeSH data available.


Related in: MedlinePlus