Carbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans.
Bottom Line: We show that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome.The regulation of cell wall structural proteins (e.g. Cht1, Phr1, Phr2, Pir1) correlated with extensive cell wall remodeling in lactate-grown cells and with their increased resistance to stresses and antifungal drugs, compared with glucose-grown cells.The analysis of the corresponding mutants confirmed that many of these proteins contribute to C. albicans adherence, stress, and antifungal drug resistance.
Affiliation: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.Show MeSH
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Mentions: Midexponential C. albicans cells were washed twice with dH2O and resuspended in PBS. A total of 107 cells/mL in 2 mL PBS were added to 12-well plates (nontreated polystyrene; Costar, Corning Inc., Ewloe, Flintshire, UK) and allowed to adhere for 1 h at 37°C without shaking. After washing three times with PBS, adhered cells were scraped off the plastic surface into 1 mL PBS and quantified by OD600 and counting colony forming units (CFUs). OD600 and CFUs displayed the same trends. Results presented in Fig. 3A and Table 2 represent the average CFUs ± SEM for at least three independent experiments for each growth condition, each with technical duplicates.
Affiliation: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.