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Carbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans.

Ene IV, Heilmann CJ, Sorgo AG, Walker LA, de Koster CG, Munro CA, Klis FM, Brown AJ - Proteomics (2012)

Bottom Line: We show that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome.The regulation of cell wall structural proteins (e.g. Cht1, Phr1, Phr2, Pir1) correlated with extensive cell wall remodeling in lactate-grown cells and with their increased resistance to stresses and antifungal drugs, compared with glucose-grown cells.The analysis of the corresponding mutants confirmed that many of these proteins contribute to C. albicans adherence, stress, and antifungal drug resistance.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.

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Related in: MedlinePlus

Carbon source affects C. albicans adherence and biofilm formation. (A) Adherence of wild type C. albicans strains grown on either glucose or lactate was assayed by measuring the number of cells (OD600 and CFUs) adhered to a plastic surface after 1 h static incubation at 37°C. Bars represent averaged CFUs ± SEM of at least three independent experiments. (B) C. albicans RM1000 cells grown in glucose or lactate display different levels of adherence to a plastic surface after 1 h. (C) Number of cells (OD600) adhered to a plastic surface (1 h, 37°C) or in biofilms (24 h, 37°C). Results represent the means ± SEM of four to six experiments, *p < 0.05 relative to glucose-grown controls. (D) Dry mass of biofilms formed on silicone in minimal medium (24 h, 37°C). Results represent the means ± SEM of ten experiments, *p < 0.05 relative to glucose-grown biofilms. (E) Morphology of cells recovered from biofilms on plastic surface grown on glucose, lactate, or glucose plus lactate (24 h, 37°C).
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fig03: Carbon source affects C. albicans adherence and biofilm formation. (A) Adherence of wild type C. albicans strains grown on either glucose or lactate was assayed by measuring the number of cells (OD600 and CFUs) adhered to a plastic surface after 1 h static incubation at 37°C. Bars represent averaged CFUs ± SEM of at least three independent experiments. (B) C. albicans RM1000 cells grown in glucose or lactate display different levels of adherence to a plastic surface after 1 h. (C) Number of cells (OD600) adhered to a plastic surface (1 h, 37°C) or in biofilms (24 h, 37°C). Results represent the means ± SEM of four to six experiments, *p < 0.05 relative to glucose-grown controls. (D) Dry mass of biofilms formed on silicone in minimal medium (24 h, 37°C). Results represent the means ± SEM of ten experiments, *p < 0.05 relative to glucose-grown biofilms. (E) Morphology of cells recovered from biofilms on plastic surface grown on glucose, lactate, or glucose plus lactate (24 h, 37°C).

Mentions: Midexponential C. albicans cells were washed twice with dH2O and resuspended in PBS. A total of 107 cells/mL in 2 mL PBS were added to 12-well plates (nontreated polystyrene; Costar, Corning Inc., Ewloe, Flintshire, UK) and allowed to adhere for 1 h at 37°C without shaking. After washing three times with PBS, adhered cells were scraped off the plastic surface into 1 mL PBS and quantified by OD600 and counting colony forming units (CFUs). OD600 and CFUs displayed the same trends. Results presented in Fig. 3A and Table 2 represent the average CFUs ± SEM for at least three independent experiments for each growth condition, each with technical duplicates.


Carbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans.

Ene IV, Heilmann CJ, Sorgo AG, Walker LA, de Koster CG, Munro CA, Klis FM, Brown AJ - Proteomics (2012)

Carbon source affects C. albicans adherence and biofilm formation. (A) Adherence of wild type C. albicans strains grown on either glucose or lactate was assayed by measuring the number of cells (OD600 and CFUs) adhered to a plastic surface after 1 h static incubation at 37°C. Bars represent averaged CFUs ± SEM of at least three independent experiments. (B) C. albicans RM1000 cells grown in glucose or lactate display different levels of adherence to a plastic surface after 1 h. (C) Number of cells (OD600) adhered to a plastic surface (1 h, 37°C) or in biofilms (24 h, 37°C). Results represent the means ± SEM of four to six experiments, *p < 0.05 relative to glucose-grown controls. (D) Dry mass of biofilms formed on silicone in minimal medium (24 h, 37°C). Results represent the means ± SEM of ten experiments, *p < 0.05 relative to glucose-grown biofilms. (E) Morphology of cells recovered from biofilms on plastic surface grown on glucose, lactate, or glucose plus lactate (24 h, 37°C).
© Copyright Policy - open-access
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3569869&req=5

fig03: Carbon source affects C. albicans adherence and biofilm formation. (A) Adherence of wild type C. albicans strains grown on either glucose or lactate was assayed by measuring the number of cells (OD600 and CFUs) adhered to a plastic surface after 1 h static incubation at 37°C. Bars represent averaged CFUs ± SEM of at least three independent experiments. (B) C. albicans RM1000 cells grown in glucose or lactate display different levels of adherence to a plastic surface after 1 h. (C) Number of cells (OD600) adhered to a plastic surface (1 h, 37°C) or in biofilms (24 h, 37°C). Results represent the means ± SEM of four to six experiments, *p < 0.05 relative to glucose-grown controls. (D) Dry mass of biofilms formed on silicone in minimal medium (24 h, 37°C). Results represent the means ± SEM of ten experiments, *p < 0.05 relative to glucose-grown biofilms. (E) Morphology of cells recovered from biofilms on plastic surface grown on glucose, lactate, or glucose plus lactate (24 h, 37°C).
Mentions: Midexponential C. albicans cells were washed twice with dH2O and resuspended in PBS. A total of 107 cells/mL in 2 mL PBS were added to 12-well plates (nontreated polystyrene; Costar, Corning Inc., Ewloe, Flintshire, UK) and allowed to adhere for 1 h at 37°C without shaking. After washing three times with PBS, adhered cells were scraped off the plastic surface into 1 mL PBS and quantified by OD600 and counting colony forming units (CFUs). OD600 and CFUs displayed the same trends. Results presented in Fig. 3A and Table 2 represent the average CFUs ± SEM for at least three independent experiments for each growth condition, each with technical duplicates.

Bottom Line: We show that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome.The regulation of cell wall structural proteins (e.g. Cht1, Phr1, Phr2, Pir1) correlated with extensive cell wall remodeling in lactate-grown cells and with their increased resistance to stresses and antifungal drugs, compared with glucose-grown cells.The analysis of the corresponding mutants confirmed that many of these proteins contribute to C. albicans adherence, stress, and antifungal drug resistance.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.

Show MeSH
Related in: MedlinePlus