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Carbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans.

Ene IV, Heilmann CJ, Sorgo AG, Walker LA, de Koster CG, Munro CA, Klis FM, Brown AJ - Proteomics (2012)

Bottom Line: We show that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome.The regulation of cell wall structural proteins (e.g. Cht1, Phr1, Phr2, Pir1) correlated with extensive cell wall remodeling in lactate-grown cells and with their increased resistance to stresses and antifungal drugs, compared with glucose-grown cells.The analysis of the corresponding mutants confirmed that many of these proteins contribute to C. albicans adherence, stress, and antifungal drug resistance.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.

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Significant changes in the wall proteome (A) and secretome (B) of C. albicans cells induced by growth on glucose, lactate, or glucose plus lactate. Differences in protein levels between the three conditions (p < 0.05) were calculated using the averaged % spectral counts from three to four independent biological replicates, each with two technical runs.
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fig02: Significant changes in the wall proteome (A) and secretome (B) of C. albicans cells induced by growth on glucose, lactate, or glucose plus lactate. Differences in protein levels between the three conditions (p < 0.05) were calculated using the averaged % spectral counts from three to four independent biological replicates, each with two technical runs.

Mentions: All secretomes and wall proteomes were highly enriched for proteins containing signal peptides. Less than 2% of all peptides identified in the secretome and cell wall proteome were of proteins without secretion signal, and the majority of wall proteins consisted of GPI proteins. These observations are consistent with previous studies [31,32,36]. Lactate- and glucose-grown C. albicans cells displayed major differences in their profiles of secreted and cell wall proteins (Fig. 2, Supporting Information Tables S1 and S2). Many of these differences indicated that the change in carbon source affected specific cellular processes associated with the cell surface, such as cell wall remodeling, adherence, biofilm formation, cell type, and metabolism. Differential protein expression in these functional categories is described below.


Carbon source-induced reprogramming of the cell wall proteome and secretome modulates the adherence and drug resistance of the fungal pathogen Candida albicans.

Ene IV, Heilmann CJ, Sorgo AG, Walker LA, de Koster CG, Munro CA, Klis FM, Brown AJ - Proteomics (2012)

Significant changes in the wall proteome (A) and secretome (B) of C. albicans cells induced by growth on glucose, lactate, or glucose plus lactate. Differences in protein levels between the three conditions (p < 0.05) were calculated using the averaged % spectral counts from three to four independent biological replicates, each with two technical runs.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569869&req=5

fig02: Significant changes in the wall proteome (A) and secretome (B) of C. albicans cells induced by growth on glucose, lactate, or glucose plus lactate. Differences in protein levels between the three conditions (p < 0.05) were calculated using the averaged % spectral counts from three to four independent biological replicates, each with two technical runs.
Mentions: All secretomes and wall proteomes were highly enriched for proteins containing signal peptides. Less than 2% of all peptides identified in the secretome and cell wall proteome were of proteins without secretion signal, and the majority of wall proteins consisted of GPI proteins. These observations are consistent with previous studies [31,32,36]. Lactate- and glucose-grown C. albicans cells displayed major differences in their profiles of secreted and cell wall proteins (Fig. 2, Supporting Information Tables S1 and S2). Many of these differences indicated that the change in carbon source affected specific cellular processes associated with the cell surface, such as cell wall remodeling, adherence, biofilm formation, cell type, and metabolism. Differential protein expression in these functional categories is described below.

Bottom Line: We show that growth on the physiologically relevant carboxylic acid, lactate, has a significant impact on the C. albicans cell wall proteome and secretome.The regulation of cell wall structural proteins (e.g. Cht1, Phr1, Phr2, Pir1) correlated with extensive cell wall remodeling in lactate-grown cells and with their increased resistance to stresses and antifungal drugs, compared with glucose-grown cells.The analysis of the corresponding mutants confirmed that many of these proteins contribute to C. albicans adherence, stress, and antifungal drug resistance.

View Article: PubMed Central - PubMed

Affiliation: Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, United Kingdom.

Show MeSH
Related in: MedlinePlus