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In vitro DNA-damaging effects of intestinal and related tetrapyrroles in human cancer cells.

Mölzer C, Pfleger B, Putz E, Roßmann A, Schwarz U, Wallner M, Bulmer AC, Wagner KH - Exp. Cell Res. (2012)

Bottom Line: Furthermore, few data suggest that tetrapyrroles exert anti-carcinogenic effects via induction of cell cycle arrest and apoptosis.Tetrapyrrole incubation mostly resulted in increased DNA-damage (comet formation) in Caco2 and HepG2 cells.Tetrapyrroles that are concentrated within the intestine, including protoporphyrin, urobilin and stercobilin, led to significant comet formation in both cell lines, implicating the compounds in inducing DNA-damage and apoptosis in cancer cells found within organs of the digestive system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Sciences, Emerging Field Oxidative Stress and DNA Stability, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. christine.moelzer@univie.ac.at

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DNA-damaging effects of (A) protoporphyrin, (B) urobilin and (C) stercobilin in HepG2 cells. ⁎n: significantly different to negative control, ⁎p: significantly different to positive control, #n: not significantly different to negative control, however strong trend (p≤0.1).
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f0010: DNA-damaging effects of (A) protoporphyrin, (B) urobilin and (C) stercobilin in HepG2 cells. ⁎n: significantly different to negative control, ⁎p: significantly different to positive control, #n: not significantly different to negative control, however strong trend (p≤0.1).

Mentions: In both cell lines, PRO at the highest and lowest tested concentrations induced significant DNA-damage versus negative control (p<0.05), as was the case in HepG2 cells, also in comparison to positive control (p<0.05;Figs. 1 and 2A). Following UB incubation in both cell lines, DNA-damaging effects tended to be or were significantly increased compared to negative control (p<0.05; Figs. 1 and 2B). In HepG2 cells, SB elevated DNA-damage compared to negative and positive controls (p<0.05; Fig. 2C).


In vitro DNA-damaging effects of intestinal and related tetrapyrroles in human cancer cells.

Mölzer C, Pfleger B, Putz E, Roßmann A, Schwarz U, Wallner M, Bulmer AC, Wagner KH - Exp. Cell Res. (2012)

DNA-damaging effects of (A) protoporphyrin, (B) urobilin and (C) stercobilin in HepG2 cells. ⁎n: significantly different to negative control, ⁎p: significantly different to positive control, #n: not significantly different to negative control, however strong trend (p≤0.1).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569715&req=5

f0010: DNA-damaging effects of (A) protoporphyrin, (B) urobilin and (C) stercobilin in HepG2 cells. ⁎n: significantly different to negative control, ⁎p: significantly different to positive control, #n: not significantly different to negative control, however strong trend (p≤0.1).
Mentions: In both cell lines, PRO at the highest and lowest tested concentrations induced significant DNA-damage versus negative control (p<0.05), as was the case in HepG2 cells, also in comparison to positive control (p<0.05;Figs. 1 and 2A). Following UB incubation in both cell lines, DNA-damaging effects tended to be or were significantly increased compared to negative control (p<0.05; Figs. 1 and 2B). In HepG2 cells, SB elevated DNA-damage compared to negative and positive controls (p<0.05; Fig. 2C).

Bottom Line: Furthermore, few data suggest that tetrapyrroles exert anti-carcinogenic effects via induction of cell cycle arrest and apoptosis.Tetrapyrrole incubation mostly resulted in increased DNA-damage (comet formation) in Caco2 and HepG2 cells.Tetrapyrroles that are concentrated within the intestine, including protoporphyrin, urobilin and stercobilin, led to significant comet formation in both cell lines, implicating the compounds in inducing DNA-damage and apoptosis in cancer cells found within organs of the digestive system.

View Article: PubMed Central - PubMed

Affiliation: Department of Nutritional Sciences, Emerging Field Oxidative Stress and DNA Stability, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090 Vienna, Austria. christine.moelzer@univie.ac.at

Show MeSH
Related in: MedlinePlus