Regulatory T-lymphocytes mediate amyotrophic lateral sclerosis progression and survival.
Bottom Line: The mRNA levels of FoxP3, TGF-β, IL4 and Gata3, a Th2 transcription factor, were reduced in rapidly progressing patients and inversely correlated with progression rates.No differences in IL10, Tbx21, a Th1 transcription factor or IFN-γ expression were found between slow and rapidly progressing patients.Importantly, early reduced FoxP3 levels could be used to identify rapidly progressing patients.
Affiliation: Department of Neurology, The Methodist Hospital Research Institute, Houston, TX, USA. firstname.lastname@example.orgShow MeSH
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Mentions: As an independent assessment, leukocyte FoxP3 and CD25 mRNA expression levels were also examined by quantitative (q)RT-PCR. FoxP3 mRNA levels from rapidly progressing patients were reduced 34% compared with slowly progressing patients (p = 0.001) and 44% compared with controls (p = 0.00002; Fig 3A). CD25 mRNA levels in leukocytes from rapidly progressing ALS patients were reduced 35% compared with slowly progressing patients (p = 0.006) and 45% compared with controls (p = 0.00003; Fig 3C). FoxP3 and CD25 mRNA levels in slowly progressing patients were not different than controls (p = 0.164, p = 0.211, respectively). Both the FoxP3 and CD25 expression levels in patients were inversely correlated with rates of progression (p = 0.003, p = 0.001, respectively; Fig 3B and D). CD25 mRNA expression levels directly correlated with FoxP3 mRNA levels (p < 0.001; Fig 3E); note that rapidly progressing patients late in their course of disease expressed the lowest levels of FoxP3 and CD25 mRNAs, whereas slowly progressing patients early in their disease expressed the highest FoxP3 and CD25 mRNA levels.
Affiliation: Department of Neurology, The Methodist Hospital Research Institute, Houston, TX, USA. email@example.com