Acute B lymphoblastic leukaemia-propagating cells are present at high frequency in diverse lymphoblast populations.
Bottom Line: Here, we demonstrate in a wide range of primary patient samples and patient samples previously passaged through mice that leukaemia-propagating cells are found in all populations defined by high or low expression of the lymphoid differentiation markers CD10, CD20 or CD34.The frequency of leukaemia-propagating cells and their engraftment kinetics do not differ between these populations.Together, these findings suggest that there is no stem cell hierarchy in acute B lymphoblastic leukaemia.
Affiliation: Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK.Show MeSH
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Mentions: Next, we tested the engraftment kinetics of the different subpopulations. To this end, we determined the leukaemic infiltration produced by CD10-, CD20- and CD34-sorted blasts over time using sequential bone marrow punctures. In order to achieve sufficient numbers of mice at each time point, all engrafted mice transplanted with either 100–300 cells or 500–3000 cells were pooled for this analysis. Each of the population engrafted with similar kinetics (Fig 4).
Affiliation: Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK.