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Acute B lymphoblastic leukaemia-propagating cells are present at high frequency in diverse lymphoblast populations.

Rehe K, Wilson K, Bomken S, Williamson D, Irving J, den Boer ML, Staa M, Schrappe M, Hall AG, Heidenreich O, Vormoor J - EMBO Mol Med (2012)

Bottom Line: Here, we demonstrate in a wide range of primary patient samples and patient samples previously passaged through mice that leukaemia-propagating cells are found in all populations defined by high or low expression of the lymphoid differentiation markers CD10, CD20 or CD34.The frequency of leukaemia-propagating cells and their engraftment kinetics do not differ between these populations.Together, these findings suggest that there is no stem cell hierarchy in acute B lymphoblastic leukaemia.

View Article: PubMed Central - PubMed

Affiliation: Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK.

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Related in: MedlinePlus

Phenotype of the re-established leukaemia after transplantation of purified B-ALL blast populationsEngraftment of primografted CD10low and CD10high cells (patient L4951).Engraftment of primografted CD20low and CD20high cells (patient EMCR1).Engraftment of primografted CD34low and CD34high cells (patient EMCR1).Engraftment of primary CD34low and CD34high cells (patient L784).
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fig02: Phenotype of the re-established leukaemia after transplantation of purified B-ALL blast populationsEngraftment of primografted CD10low and CD10high cells (patient L4951).Engraftment of primografted CD20low and CD20high cells (patient EMCR1).Engraftment of primografted CD34low and CD34high cells (patient EMCR1).Engraftment of primary CD34low and CD34high cells (patient L784).

Mentions: We have previously suggested a model of malleability whereby different B-ALL populations sorted for a specific surface marker could re-establish their phenotypic counterparts in vivo. Here, we confirm this malleability for the B-cell differentiation markers CD10, CD20 and CD34 in mice transplanted with purified cells at low cell doses. Fig 2A–C shows the flow analysis of leukaemias initiated by blasts sorted for high and low expression of CD10, CD20 or CD34. All populations were able to reconstitute their corresponding population in vivo. The same picture was observed in mice transplanted with cells purified from primary samples: CD20low and CD20high as well as CD34low and CD34high cells (Fig 2D) engrafted and were able to reproduce both populations.


Acute B lymphoblastic leukaemia-propagating cells are present at high frequency in diverse lymphoblast populations.

Rehe K, Wilson K, Bomken S, Williamson D, Irving J, den Boer ML, Staa M, Schrappe M, Hall AG, Heidenreich O, Vormoor J - EMBO Mol Med (2012)

Phenotype of the re-established leukaemia after transplantation of purified B-ALL blast populationsEngraftment of primografted CD10low and CD10high cells (patient L4951).Engraftment of primografted CD20low and CD20high cells (patient EMCR1).Engraftment of primografted CD34low and CD34high cells (patient EMCR1).Engraftment of primary CD34low and CD34high cells (patient L784).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3569652&req=5

fig02: Phenotype of the re-established leukaemia after transplantation of purified B-ALL blast populationsEngraftment of primografted CD10low and CD10high cells (patient L4951).Engraftment of primografted CD20low and CD20high cells (patient EMCR1).Engraftment of primografted CD34low and CD34high cells (patient EMCR1).Engraftment of primary CD34low and CD34high cells (patient L784).
Mentions: We have previously suggested a model of malleability whereby different B-ALL populations sorted for a specific surface marker could re-establish their phenotypic counterparts in vivo. Here, we confirm this malleability for the B-cell differentiation markers CD10, CD20 and CD34 in mice transplanted with purified cells at low cell doses. Fig 2A–C shows the flow analysis of leukaemias initiated by blasts sorted for high and low expression of CD10, CD20 or CD34. All populations were able to reconstitute their corresponding population in vivo. The same picture was observed in mice transplanted with cells purified from primary samples: CD20low and CD20high as well as CD34low and CD34high cells (Fig 2D) engrafted and were able to reproduce both populations.

Bottom Line: Here, we demonstrate in a wide range of primary patient samples and patient samples previously passaged through mice that leukaemia-propagating cells are found in all populations defined by high or low expression of the lymphoid differentiation markers CD10, CD20 or CD34.The frequency of leukaemia-propagating cells and their engraftment kinetics do not differ between these populations.Together, these findings suggest that there is no stem cell hierarchy in acute B lymphoblastic leukaemia.

View Article: PubMed Central - PubMed

Affiliation: Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK.

Show MeSH
Related in: MedlinePlus