Acute B lymphoblastic leukaemia-propagating cells are present at high frequency in diverse lymphoblast populations.
Bottom Line: Here, we demonstrate in a wide range of primary patient samples and patient samples previously passaged through mice that leukaemia-propagating cells are found in all populations defined by high or low expression of the lymphoid differentiation markers CD10, CD20 or CD34.The frequency of leukaemia-propagating cells and their engraftment kinetics do not differ between these populations.Together, these findings suggest that there is no stem cell hierarchy in acute B lymphoblastic leukaemia.
Affiliation: Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK.Show MeSH
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Mentions: We have previously suggested a model of malleability whereby different B-ALL populations sorted for a specific surface marker could re-establish their phenotypic counterparts in vivo. Here, we confirm this malleability for the B-cell differentiation markers CD10, CD20 and CD34 in mice transplanted with purified cells at low cell doses. Fig 2A–C shows the flow analysis of leukaemias initiated by blasts sorted for high and low expression of CD10, CD20 or CD34. All populations were able to reconstitute their corresponding population in vivo. The same picture was observed in mice transplanted with cells purified from primary samples: CD20low and CD20high as well as CD34low and CD34high cells (Fig 2D) engrafted and were able to reproduce both populations.
Affiliation: Newcastle Cancer Centre at the Northern Institute for Cancer Research, Newcastle University, Newcastle upon Tyne, UK.