Skin-draining lymph node priming is sufficient to induce sterile immunity against pre-erythrocytic malaria.
Bottom Line: However, it has recently been demonstrated that priming also occurs in the skin.We wished to establish if sterile protection could be obtained in the absence of priming by infected hepatocytes.We then compared and contrasted the patterns of priming with those obtained by intradermal immunization, where priming occurs in the liver.
Affiliation: Inserm, UMR-S 945, Paris, France. email@example.comShow MeSH
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Mentions: In order to minimize the circulation of activated T cells that is likely to occur with repeated boosting doses, we adopted an effective vaccination regimen of a single s.c. inoculation 600 × 103 γ-spz (Table 1). Groups of mice were immunized s.c. or i.d. with γ-spz, and one set was treated with DT whereas the other served as control. Cells from the iLN of control s.c.- or i.d.-immunized mice (i.e. not CD11c-depleted) produced high levels of interferon-γ in response to stimulus by a CSP epitope. In contrast, iLN cells from DT-injected immunized mice (i.e. CD11c DC-depleted) did not produce interferon-γ in response to the same stimulus (Fig 3A). Furthermore, the sterile protection conferred by immunization was completely abrogated by CD11c DC depletion (Fig 3B). Finally, adoptive transfer of iLN cells from control immunized mice protected recipient naïve mice against PyWT sporozoite challenge, but the iLN cells from CD11c DC-depleted immunized mice failed to do so (Fig 3C).
Affiliation: Inserm, UMR-S 945, Paris, France. firstname.lastname@example.org